Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,260,338	T→A	8.5%	Q161L (CAG→CTG)	PP_2857 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,260,338	0	T	A	8.5%	193.5 / 3.9	67	Q161L (CAG→CTG)	PP_2857	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base A (3/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTGATTCTCCGTACGCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCT  >  NC_002947/3260191‑3260485                                                                                                                                                     |                                                                                                                                                    tGGTGATTCTCCGTACGCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTg                                                                                                                                                    <  5:53745/149‑1 (MQ=255)   gTGATTCTCCGTACGCTCTTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGt                                                                                                                                                  <  4:202261/149‑1 (MQ=255)        tctcCGTACGCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTg                                                                                                                                             >  8:134996/1‑149 (MQ=255)            cGTACGCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTgg                                                                                                                                            <  7:115830/146‑1 (MQ=255)            cGTACGCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAg                                                                                                                                         >  5:234431/1‑149 (MQ=255)                 gCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGAcc                                                                                                                                    <  6:128018/149‑1 (MQ=255)                   tATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGAccag                                                                                                                                  >  2:341061/1‑147 (MQ=255)                   tATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTAGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCATCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGAc                                                                                                                                     >  7:139465/1‑146 (MQ=255)                     ttAGGTTTAGTGTCGTTGTTAAATGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGAcca                                                                                                                                   <  8:139465/146‑2 (MQ=255)                     ttAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTg                                                                                                                                  <  8:172541/147‑1 (MQ=255)                     ttAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGAccag                                                                                                                                  <  2:279227/147‑3 (MQ=255)                       aGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGACCAGGcc                                                                                                                               <  1:341061/148‑2 (MQ=255)                                 tCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCctgctg                                                                                                                    <  7:134996/149‑1 (MQ=255)                                    ttCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGaa                                                                                                                 <  6:297177/149‑1 (MQ=255)                                    ttCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGaa                                                                                                                 <  3:209580/149‑1 (MQ=255)                                    tgattAACTGCCGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGaa                                                                                                                 <  6:234431/146‑1 (MQ=255)                                            tGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGtt                                                                                                         >  1:266817/1‑149 (MQ=255)                                              cAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGtt                                                                                                         >  2:174358/1‑147 (MQ=255)                                              cAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGtt                                                                                                         >  2:179846/1‑147 (MQ=255)                                              cAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGtt                                                                                                         <  1:179846/147‑1 (MQ=255)                                              cAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGtt                                                                                                         <  1:174358/147‑1 (MQ=255)                                                gggTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTc                                                                                                     <  1:356921/149‑1 (MQ=255)                                                           cgACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGttgttg                                                                                          <  8:327371/149‑1 (MQ=255)                                                                       tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACGTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGcc                                                                                                                         >  1:238924/1‑107 (MQ=255)                                                                       tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGcc                                                                                                                         <  2:238924/106‑1 (MQ=255)                                                                       tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACg                                                                              >  8:217284/1‑149 (MQ=255)                                                                       tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACg                                                                              >  2:25337/1‑149 (MQ=255)                                                                       tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACg                                                                              >  8:275096/1‑149 (MQ=255)                                                                         aaGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGtt                                                                            >  1:166224/1‑149 (MQ=255)                                                                               ttGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGtt                                                                            >  5:130443/1‑143 (MQ=255)                                                                               ttGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGAGCCACGtt                                                                            <  6:130443/143‑1 (MQ=255)                                                                                 ggCCATTCGGTTTCCCACCCCCGCAC‑TCTCCTTCAACTGGAACACCCCTCGGCTACCGGTGAAGGCCAGGTCACTGGTAGTGACCTTGCGAAAGCATGCTGGATTGGCAGTTGCTCCTGAGTTGGTGATCAGCGACACGTTCTGTTGCa                                                                    >  7:9399/1‑149 (MQ=255)                                                                                     aTTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAg                                                                >  7:118427/1‑149 (MQ=255)                                                                                     aTTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAg                                                                >  3:142127/1‑149 (MQ=255)                                                                                     aTTCGGTTTCCCACCCCGGCAC‑TCTGGTTAAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAg                                                                >  2:316861/1‑149 (MQ=255)                                                                                         ggTTTCCCACCCCGGCAC‑TCTTGTTCAACTGGATCACCCCTCGGCTACCTTTGAAGGCCTGGTCACTGTTAGTGACCTGGAGATAGCCTTCTTGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGAc                                                             <  3:299929/148‑1 (MQ=255)                                                                                           tttCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGTCTACCGGTGAAGGCCTTGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCt                                                          <  6:182170/149‑1 (MQ=255)                                                                                              cccACCCCGGCAG‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCAGTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtc                                                       >  6:173983/1‑149 (MQ=255)                                                                                               ccACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGAcc                                                                                                                                    >  6:94911/1‑71 (MQ=255)                                                                                               ccACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGAcc                                                                                                                                    <  5:94911/71‑1 (MQ=255)                                                                                                cACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtcgt                                                     >  1:314396/1‑149 (MQ=255)                                                                                                cACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGAGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTGGTTGATCAGCGCCACCTTCAGTTGCATGAGGACGCTgtcgt                                                     >  7:79680/1‑149 (MQ=255)                                                                                                  ccccGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtcgtc                                                    >  4:28976/1‑148 (MQ=255)                                                                                                     cGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATg                                                <  8:302879/149‑1 (MQ=255)                                                                                                     cGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATg                                                <  1:316861/149‑1 (MQ=255)                                                                                                              tGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtcgt                                                     >  1:197642/1‑136 (MQ=255)                                                                                                              tGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtcgt                                                     <  2:197642/136‑1 (MQ=255)                                                                                                              tGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCgg                                        >  1:93676/1‑149 (MQ=255)                                                                                                               ggTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGt                                       >  2:80381/1‑149 (MQ=255)                                                                                                                gTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCg                                         <  4:142127/146‑1 (MQ=255)                                                                                                                  tCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGt                                                                        <  4:304187/113‑1 (MQ=255)                                                                                                                  tCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGt                                                                        >  3:304187/1‑113 (MQ=255)                                                                                                                   cAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTtgt                                    <  7:275096/148‑1 (MQ=255)                                                                                                                   cAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTtgt                                    <  2:266817/148‑1 (MQ=255)                                                                                                                         gATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCaa                             <  2:166224/149‑1 (MQ=255)                                                                                                                               cccTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGtt                                      >  4:349469/1‑134 (MQ=255)                                                                                                                               cccTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGtt                                      <  3:349469/134‑1 (MQ=255)                                                                                                                                ccTCGTCTACCGGTGAAGGCCTGGTCTCTGGTAGTGACCGGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTGGTTGATCAGCGGCAGGTTCTGTTGCATGAGGAGGCTGTCGTCGATGCTTTGCGGTTGTGCAGCAATGCTGAt                      <  8:9399/149‑1 (MQ=255)                                                                                                                                ccTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGATGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGAt                      >  1:108054/1‑149 (MQ=255)                                                                                                                                  tCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTg                        <  2:24304/145‑1 (MQ=255)                                                                                                                                  tCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTg                        >  1:24304/1‑145 (MQ=255)                                                                                                                                       tACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAg                                                                                                <  7:61710/68‑1 (MQ=255)                                                                                                                                       tACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAg                                                                                                >  8:61710/1‑68 (MQ=255)                                                                                                                                            gTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGtt          <  7:217284/149‑1 (MQ=255)                                                                                                                                                    cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTGAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCTATGCTGATGCTGAGTGCGTTggctggct  >  4:220224/1‑149 (MQ=255)                                                                                                                                                    cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTtgt                                    >  6:9675/1‑115 (MQ=255)                                                                                                                                                    cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTtgt                                    <  5:9675/115‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGGTGATTCTCCGTACGCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCAC‑TCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCT  >  NC_002947/3260191‑3260485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑