| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,268,089 | C→T | 6.9% | A187V (GCG→GTG) | azoR1 → | FMN‑dependent NADH‑azoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,268,089 | 0 | C | T | 6.9% | 171.6 / 2.1 | 58 | A187V (GCG→GTG) | azoR1 | FMN‑dependent NADH‑azoreductase |
| Reads supporting (aligned to +/- strand): ref base C (24/30); new base T (2/2); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
CACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGAT > NC_002947/3267943‑3268232 | cACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcga < 3:100780/149‑1 (MQ=255)cACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcga < 3:95821/149‑1 (MQ=255) gTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGTGATCGAtgct > 6:302208/1‑149 (MQ=255) gTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGTGATCGAtgct > 6:212240/1‑149 (MQ=255) cgatGGCCAGCCGACTGGAGCCGGGCATGAAGACTTCCTGAAACTGTTCATGCGCTTCATCGGGATTACTGACCTGGAAATATTCCGGGCAAACGGCCTGGCCTATGGAACGGAGCAGCGCAGCCAGGCTATCGATGCTGCACAGgcg > 8:68123/4‑148 (MQ=255) cGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcgc < 7:295581/149‑1 (MQ=255) cGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcgc < 5:64467/149‑1 (MQ=255) gCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATTGGGATTACTGACCTGGAAATCGTCCGGGCCCATGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGcac < 8:127094/142‑1 (MQ=255) gCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATTGGGATTACTGACCTGGAAATCGTCCGGGCCCATGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGcac > 7:127094/1‑142 (MQ=255) cTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAg < 5:358432/149‑1 (MQ=255) gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGTGATCGATGCTGCACAGGCGCAGAt < 5:212240/148‑1 (MQ=255) gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGTGATCGATGCTGCACAGGCGCAGAt < 5:302208/148‑1 (MQ=255) gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCGTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGAtt < 4:70209/149‑1 (MQ=255) cAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGc < 5:47111/149‑1 (MQ=255) cGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGc < 7:68123/149‑1 (MQ=255) cATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGc > 3:184212/1‑149 (MQ=255) gACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGt < 2:14739/136‑1 (MQ=255) gACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGt > 1:14739/1‑136 (MQ=255) ttCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGAt > 5:286488/1‑148 (MQ=255) gtTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGtt > 6:111101/1‑149 (MQ=255) tGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGtt < 1:193377/118‑1 (MQ=255) tGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGtt > 2:193377/1‑118 (MQ=255) tGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTgg < 1:215990/147‑1 (MQ=255) tGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTgg > 2:215990/1‑147 (MQ=255) ggCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGc < 5:137188/149‑1 (MQ=255) tCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTg > 4:78108/1‑149 (MQ=255) ggATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGcc < 6:337157/149‑1 (MQ=255) ggATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGcc < 6:286488/149‑1 (MQ=255) gATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatcga > 8:91115/1‑72 (MQ=255) gATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatcga < 7:91115/72‑1 (MQ=255) aCTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGt < 3:18275/148‑1 (MQ=255) cggACCTGGAAAGCCGGTGGGGCCACGGCCTGGCCTATGGCGCGGAGCAGCGCAGGCTGTCGATCGATGCTGCAGAGGCTCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGAGCTTGGGAGCGTTTGTTTGCAAGCGTGGCCATGTc < 8:238138/146‑1 (MQ=255) tGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcgc > 6:95320/1‑80 (MQ=255) tGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcgc < 5:95320/80‑1 (MQ=255) tGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGcc < 8:312006/142‑1 (MQ=255) tGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGcc > 7:312006/1‑142 (MQ=255) cTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTgg > 5:198836/1‑136 (MQ=255) cTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTgg < 6:198836/136‑1 (MQ=255) ggAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGa > 7:94176/1‑143 (MQ=255) ggAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGa < 8:94176/143‑1 (MQ=255) aaaTCGTCCGGGCCCAAGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAg < 4:184212/149‑1 (MQ=255) tgctCCGGGCCATAGGCCAGGCCGTGGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGc > 4:195671/4‑148 (MQ=255) gTCCGTGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGaaa < 3:127995/138‑1 (MQ=255) gTCCGGGCCCAAGCCCTGGCCTATGGCCCGTAGCAGAGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATAGCCAGCGAGCTGTTTGCGGATGCGTGATCAAGCGACCGGTTGGTTGCAAGCGTGGCCCTGTCGaaa > 4:127995/1‑138 (MQ=255) ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGc > 6:262831/1‑115 (MQ=255) ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGc < 7:265788/115‑1 (MQ=255) ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGc > 8:265788/1‑115 (MQ=255) ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGc < 5:262831/115‑1 (MQ=255) ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCt > 5:252047/1‑149 (MQ=255) aCGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTg > 2:209712/1‑148 (MQ=255) ggccttgcctATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAg < 6:252047/149‑1 (MQ=255) cctggcctATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGaa < 4:300473/149‑1 (MQ=255) aTGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTg < 5:256136/110‑1 (MQ=255) aTGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTg > 6:256136/1‑110 (MQ=255) cagcgcagcCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGAt < 7:178712/149‑1 (MQ=255) cgcagcCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAg > 5:258091/1‑135 (MQ=255) cgcagcCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAg < 6:258091/135‑1 (MQ=255) cgcagcCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAg > 7:89305/1‑149 (MQ=255) gcagcCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGc < 5:111101/149‑1 (MQ=255) ccAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGt < 4:273524/118‑1 (MQ=255) ccAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGt > 3:273524/1‑118 (MQ=255) aGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGAt > 7:306637/1‑147 (MQ=255) | CACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAGCGTTCAGGCCACCGGGATGAGCGGAT > NC_002947/3267943‑3268232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |