Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,289,727	T→A	8.2%	T253T (ACT→ACA)	PP_2892 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,289,727	0	T	A	8.2%	165.0 / 2.9	57	T253T (ACT→ACA)	PP_2892	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base A (3/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACACACCGCTGGACGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTGGGTCAACCCACAAGGCCA  >  NC_002947/3289581‑3289873                                                                                                                                                   |                                                                                                                                                    gACACACCGCTGGACGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCActgc                                                                                                                                                   >  6:195717/1‑149 (MQ=255)    acacCGCTGGACGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCActgctgc                                                                                                                                                >  1:7459/1‑149 (MQ=255)      acCGCTGGACGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCActgctgctg                                                                                                                                              >  8:20244/1‑149 (MQ=255)        cgcgGGACGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCtgtg                                                                                                                                            <  8:54445/149‑1 (MQ=255)             gtcGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCga                                                                                                                                       <  8:285880/147‑1 (MQ=255)              aCGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCgaa                                                                                                                                      <  7:20244/149‑1 (MQ=255)               cGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCgaag                                                                                                                                     <  5:195717/149‑1 (MQ=255)                         ctgcAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCgg                                                                                                                            <  4:340847/148‑1 (MQ=255)                                  aTGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGt                                                                                                                           <  4:325712/140‑1 (MQ=255)                                  aTGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGt                                                                                                                           >  3:325712/1‑140 (MQ=255)                                  aTGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAACGCGTGCGCGTGGATGCGGCGCCGACATCTGCTGTGGGCTAAGAAGTCCCGGTGGATGTTc                                                                                                                   >  8:128750/1‑148 (MQ=255)                                      tgtCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTg                                                                                                              <  3:1569/149‑1 (MQ=255)                                        tCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCCCTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTgg                                                                                                             >  5:349194/1‑148 (MQ=255)                                        tCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTgg                                                                                                             >  1:40863/1‑148 (MQ=255)                                          ggtCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGaaa                                                                                                          <  8:181925/147‑1 (MQ=255)                                          gTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTg                                                                                                                          >  5:112007/1‑133 (MQ=255)                                          gTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTg                                                                                                                          <  6:112007/133‑1 (MQ=255)                                           ttCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACAGCAGCAGTGGGCgc                                                                                                                                       <  5:61599/119‑2 (MQ=255)                                           ttCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACAGCAGCAGTGGGCgc                                                                                                                                       >  6:61599/1‑118 (MQ=255)                                                gCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGt                                                                                                     >  6:112428/1‑148 (MQ=255)                                                       aCCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTggg                                                                                                                                          >  5:369707/1‑104 (MQ=255)                                                       aCCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTggg                                                                                                                                          <  6:369707/104‑1 (MQ=255)                                                        ccccTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGc                                                                                            <  1:261894/149‑1 (MQ=255)                                                          ccTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCAc                                                                                          <  2:7459/149‑1 (MQ=255)                                                               cGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATgg                                                                                     <  3:160417/149‑1 (MQ=255)                                                                            gTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTgg                                                                         <  3:71088/148‑1 (MQ=255)                                                                               gCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCtt                                                                     >  3:41246/1‑149 (MQ=255)                                                                               gCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCtt                                                                     >  4:130538/1‑149 (MQ=255)                                                                               gCAGAGCTGAACAACGACCTGCAAGCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCtt                                                                     >  6:126858/1‑149 (MQ=255)                                                                                       gAACAACGACCTGCAAACGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCCGTGGATGTTCATCTGGAAACCCGTAAACCAAGCACCATGGGCCTTGGCCTGGGCTTCac‑cacc                                                             >  8:55266/1‑148 (MQ=255)                                                                                        aacaacGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTc‑gaccga                                                           >  4:96538/1‑149 (MQ=255)                                                                                             cGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCg                                                      >  2:168164/1‑149 (MQ=255)                                                                                              gACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGt                                                                                                                           >  5:133683/1‑80 (MQ=255)                                                                                              gACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGt                                                                                                                           <  6:133683/80‑1 (MQ=255)                                                                                              gACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCgg                                                     >  3:142405/1‑149 (MQ=255)                                                                                                  tGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCcg                                                 >  2:263723/1‑149 (MQ=255)                                                                                                    cAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAACCCACGCACCATGGGCCTTGGCCTGGGCTGCTC‑GCCCGCCGGCGGGCcgcg                                               >  5:361206/1‑149 (MQ=255)                                                                                                     aaTCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCcgcgc                                              >  4:246125/1‑149 (MQ=255)                                                                                                         gAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGc                                           >  3:99062/1‑148 (MQ=255)                                                                                                          aGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGc                                           >  3:266214/1‑147 (MQ=255)                                                                                                           gCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAg                                        <  2:40863/149‑1 (MQ=255)                                                                                                            cGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAgg                                       >  8:332292/1‑149 (MQ=255)                                                                                                             ggCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGc                                      <  5:126858/149‑1 (MQ=255)                                                                                                                     cGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGc                                      >  4:249689/1‑141 (MQ=255)                                                                                                                     cGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGc                                      <  3:249689/141‑1 (MQ=255)                                                                                                                              gcgcGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACt                     <  3:246125/149‑1 (MQ=255)                                                                                                                               cgcgTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCCAGGCCAACTGGACCCGCCACTg                    <  7:55266/149‑1 (MQ=255)                                                                                                                                gcgTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGcc                                                  >  1:127716/1‑118 (MQ=255)                                                                                                                                gcgTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGcc                                                  <  2:127716/118‑1 (MQ=255)                                                                                                                                gcgTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTgg                   <  5:112428/149‑1 (MQ=255)                                                                                                                                 cgTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTggg                  <  7:332292/149‑1 (MQ=255)                                                                                                                                 cgTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTggg                  <  5:23032/149‑1 (MQ=255)                                                                                                                                  gTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTCAAAGGGGCGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTggg                  >  1:298482/1‑149 (MQ=255)                                                                                                                                  gTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTGGGt                 <  3:96538/149‑1 (MQ=255)                                                                                                                                      aTGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTGGGTCAAc             <  7:128750/149‑1 (MQ=255)                                                                                                                                           tcgcCCACAGCAGCAGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGAc                             >  2:22583/2‑128 (MQ=255)                                                                                                                                           tcgcCCACAGCAGCAGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGAc                             <  1:22583/127‑1 (MQ=255)                                                                                                                                                 aCTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTgg                   <  6:158374/132‑1 (MQ=255)                                                                                                                                                 aCTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTgg                   >  5:158374/1‑132 (MQ=255)                                                                                                                                                 aCTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTGGGTCAACCCACAAGGCca  >  7:200492/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GACACACCGCTGGACGAGGACCTGCTGCAGCGCATGGTGTCGTTCAAGCCGGGTACCCCTTACGACTCGGAACTGGTCGCAGAGCTGAACAACGACCTGCAATCGAGCGGCTATTTCGAAGGCGTGCGCGTGGATGCGGCGCCCACTGCTGCTGTGGGCGAAGAAGTCCCGGTGGATGTTCATCTGGAAACCCGTAAACCACGCACCATGGGCCTTGGCCTGGGCTTCTC‑GACCGACGTCGGGCCGCGCGGCAAGGCCAACTGGACCCGCCACTGGGTCAACCCACAAGGCCA  >  NC_002947/3289581‑3289873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑