Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,312,689 T→A 8.9% intergenic (+30/‑132) PP_2912 → / → hemB hypothetical protein/5‑aminolevulinate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,312,6890TA 8.9% 124.5 / 3.2 45intergenic (+30/‑132)PP_2912/hemBhypothetical protein/5‑aminolevulinate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base A (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GTGATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAA  >  NC_002947/3312543‑3312832
                                                                                                                                                  |                                                                                                                                               
gTGATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTAc                                                                                                                                               <  1:1155/149‑1 (MQ=255)
   aTGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTAccc                                                                                                                                             <  8:168709/148‑1 (MQ=255)
     gCGTGAGATGGACGCCGCCCGGATGGACGACGCTCGGCGCGGTGCTGCACGCGCCGAGTCCGAGCATGGTCCGGCCAAGGCAGAGTAGGTAACGCCGCCCTGGCTGCAGTATTTTATTCCTGTACCGCCTCTTCGGGTGGTTACCcgc                                                                                                                                           <  4:119220/148‑1 (MQ=255)
       gTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGaa                                                                                                                                        <  5:300164/149‑1 (MQ=255)
          agaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGTAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGTAACCCCGCGAaga                                                                                                                                      <  3:291223/148‑1 (MQ=255)
          agaTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCGGCAGTAGTTAATTCCTGTACCGCCTCTGCGCGGGGTTACCCGCGCagag                                                                                                                                     >  7:64964/1‑149 (MQ=255)
            aTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAagag                                                                                                                                     <  3:41669/147‑1 (MQ=255)
            aTCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAagag                                                                                                                                     >  4:41669/1‑147 (MQ=255)
                 cgccgcCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAaca                                                                                                                              <  1:211524/149‑1 (MQ=255)
                    cgccCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAg                                                                                                                           <  4:316602/149‑1 (MQ=255)
                               acgacgCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAgg                                                                                                                          <  1:287558/139‑1 (MQ=255)
                               acgacgCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAgg                                                                                                                          >  2:287558/1‑139 (MQ=255)
                               acgacgCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACgg                                                                                                                >  4:295084/1‑149 (MQ=255)
                                    gCTCGTCGCCGTGCAGCTCGCGCCGATGCCGAGCATGGGCCGGGCATGGCAGAGGAGGTCACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGGGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGtt                                                                                                           <  8:64964/149‑1 (MQ=255)
                                    gCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGtt                                                                                                           <  7:111393/149‑1 (MQ=255)
                                            ccGTGCAGCACGCGCCGAGGCCGAGCATGGACCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCcg                                                                                                                                            >  8:21701/1‑108 (MQ=255)
                                            ccGTGCAGCACGCGCCGAGGCCGAGCATGGACCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCcg                                                                                                                                            <  7:21701/108‑1 (MQ=255)
                                                   gcaCGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGa                                                                                            <  1:243230/149‑1 (MQ=255)
                                                           cgaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCcg                                                                                                                                            >  3:144253/1‑93 (MQ=255)
                                                           cgaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCcg                                                                                                                                            <  4:144253/93‑1 (MQ=255)
                                                           cgaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATtct                                                                                    >  5:20484/1‑149 (MQ=255)
                                                            gaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATtctc                                                                                   <  3:741/149‑1 (MQ=255)
                                                            gaggccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCCGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATtctc                                                                                   <  1:141068/149‑1 (MQ=255)
                                                                ccgagCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCa                                                                               <  3:295084/149‑1 (MQ=255)
                                                                          gccggccAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTATTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCttt                                                                     >  8:314650/1‑149 (MQ=255)
                                                                                 aaGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCACGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTgg                                                              >  2:245718/1‑149 (MQ=255)
                                                                                        aggaggTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGa                                                       <  6:20484/149‑1 (MQ=255)
                                                                                          gaggTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCACGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACa                                                     <  1:245718/149‑1 (MQ=255)
                                                                                             gTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGCCGCAAGCCACAATCTGGTGATTTAGCCTTTTGACTGGCGCGCGACAgg                                                   >  4:210781/1‑148 (MQ=255)
                                                                                                   ccgccCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAg                                                                          >  5:24281/1‑119 (MQ=255)
                                                                                                   ccgccCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAg                                                                          <  6:24281/119‑1 (MQ=255)
                                                                                                                            cTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGCCACCTTCTCGTCATTTAGCCTTTTCCCTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAg                   >  5:347675/1‑149 (MQ=255)
                                                                                                                            cTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGa                    <  4:129378/148‑1 (MQ=255)
                                                                                                                             tGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTg                                                               >  4:242228/1‑104 (MQ=255)
                                                                                                                                  cGCCTCTTCGCGGGGTTACCCGCGAAGAGGCAAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCa             >  1:318850/1‑149 (MQ=255)
                                                                                                                                     ctctTCGCGGGTAACCCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGt          >  8:97732/1‑149 (MQ=255)
                                                                                                                                      tctTCGCGGGTAACCCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTg                      >  6:82133/1‑136 (MQ=255)
                                                                                                                                      tctTCGCGGGTAACCCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTg                      <  5:82133/136‑1 (MQ=255)
                                                                                                                                      tctTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCt                       <  4:142553/135‑1 (MQ=255)
                                                                                                                                      tctTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCt                       >  3:142553/1‑135 (MQ=255)
                                                                                                                                      tctTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTc         >  8:235966/1‑149 (MQ=255)
                                                                                                                                        ttCGCGGGGTTACCCGCGAAGAGGCCAACACATGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTc         <  7:314650/147‑1 (MQ=255)
                                                                                                                                         tCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATaa                                              <  2:142801/109‑1 (MQ=255)
                                                                                                                                         tCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATaa                                              >  1:142801/1‑109 (MQ=255)
                                                                                                                                             ggggTTATCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTccaaccaa  >  5:354312/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTGATGCGTGAGATCGACGCCGCCCGGATGGACGACGCTCGTCGCCGTGCAGCACGCGCCGAGGCCGAGCATGGGCCGGCCAAGGCAGAGGAGGTAACGCCGCCCTGGCTGCAGTAGTTTATTCCTGTACCGCCTCTTCGCGGGGTTACCCGCGAAGAGGCCAACACAGGCAAACCACGGCCGTTGCCATTGACGCAAGACACATTCTCGTCATTTAGCCTTTTCACTGGCGCGCGACAGGCATAAGGTATACGCCGTTCAAATTCCCTGGAGCTGTCATGTCCAACCAA  >  NC_002947/3312543‑3312832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑