Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,328,670 T→C 9.7% V74A (GTC→GCC)  PP_2927 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,328,6700TC 9.7% 105.4 / 2.6 41V74A (GTC→GCC) PP_2927hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/20);  new base C (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

ATTCTCGGCGATGAACTGGGGCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCATGACAAGGACAAGCTGGTAGCCGCCATG  >  NC_002947/3328528‑3328809
                                                                                                                                              |                                                                                                                                           
aTTCTCGGCGATGAACTGGGGCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGa                                                                                                                                       <  4:275290/149‑1 (MQ=255)
         gATGAACTGGGGCTGTACCGGGCCATGGCCGACCGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGCCCATGACAAGGGCCg                                                                                                                              >  1:191353/1‑149 (MQ=255)
         gATGAACTGGGGCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGCCCATGACAAGGGCCg                                                                                                                              >  1:197030/1‑149 (MQ=255)
           tGAACTGGGGCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGtt                                                                                                                            <  4:93342/149‑1 (MQ=255)
              aCTGGGGCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTtg                                                                                                                         <  4:53878/149‑1 (MQ=255)
                   ggCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGtgctg                                                                                                                    <  5:75148/149‑1 (MQ=255)
                         aCCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACa                                                                                                                                     >  2:123406/1‑126 (MQ=255)
                          ccGGGACATGGCCGACAGCCAGGCGGTCACCCCTTATCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTgaa                                                                                                              <  8:154422/148‑1 (MQ=255)
                                          aggcAGGCTGTCTCCGCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAAGAGGCCATGGCCCt                                                                                             <  2:354237/146‑1 (MQ=255)
                                              aGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAg                                                                                                                                   >  1:143871/1‑107 (MQ=255)
                                              aGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAg                                                                                                                                   <  2:143871/107‑1 (MQ=255)
                                                 cGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTg                                                                                      >  7:111466/1‑149 (MQ=255)
                                                   gTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTgga                                                                                    <  3:320721/149‑1 (MQ=255)
                                                   gTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTCCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTgga                                                                                    <  6:333150/149‑1 (MQ=255)
                                                      aCCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTggagga                                                                                 <  8:111466/149‑1 (MQ=255)
                                                        cccTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACt                                                                               >  3:193150/1‑149 (MQ=255)
                                                                aaCTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGt                                                                                                                                             <  2:313960/79‑1 (MQ=255)
                                                                aaCTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGt                                                                                                                                             >  1:313960/1‑79 (MQ=255)
                                                                 aCTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCg                                                                          >  2:28147/1‑145 (MQ=255)
                                                                 aCTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCg                                                                          <  1:28147/145‑1 (MQ=255)
                                                                     gCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCAGGGCCCTGGCGCTGGAGGACTCCCCGGCCTACAt                                                                  >  8:322838/1‑149 (MQ=255)
                                                                        gAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTggagg                                                                                  >  6:112132/1‑130 (MQ=255)
                                                                        gAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTggagg                                                                                  <  5:112132/130‑1 (MQ=255)
                                                                         aacaGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGcc                                                              <  7:317148/146‑1 (MQ=255)
                                                                          aaaGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCcg                                                             >  2:345963/1‑149 (MQ=255)
                                                                             gACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCcggcg                                                          <  1:178260/149‑1 (MQ=255)
                                                                                   cTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCggcggggcgg                                                    >  7:333800/1‑149 (MQ=255)
                                                                                      ccACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAg                                                 <  1:345963/149‑1 (MQ=255)
                                                                                       cACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAgt                                                >  6:54147/1‑149 (MQ=255)
                                                                                        aCCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGCTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAgtg                                               <  7:322838/149‑1 (MQ=255)
                                                                                            cgagCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGtggtgg                                           >  1:187036/4‑149 (MQ=255)
                                                                                                         gaATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGCCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCa                              <  2:197030/149‑1 (MQ=255)
                                                                                                         gaATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGCCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCa                              <  2:191353/149‑1 (MQ=255)
                                                                                                           aTGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCAt                             <  4:193150/148‑1 (MQ=255)
                                                                                                           aTGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCAt                             <  2:187036/148‑1 (MQ=255)
                                                                                                               ttGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCcggc                                                           <  3:76343/114‑1 (MQ=255)
                                                                                                               ttGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCcggc                                                           >  4:76343/1‑114 (MQ=255)
                                                                                                                 gAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCATgacaag                       >  3:230265/1‑148 (MQ=255)
                                                                                                                                   gggCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCATGACAAGGACAAGCTGGTAGCCGCCa    >  5:204794/1‑149 (MQ=255)
                                                                                                                                     gCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCATGACAAGGACAAGCTGGTAGCCGCCATg  >  3:2291/1‑149 (MQ=255)
                                                                                                                                     gCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCATGACAAGGACAAGCTGGTAGCCGCCATg  >  4:280822/1‑149 (MQ=255)
                                                                                                                                         cATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTAc                                                                    >  6:313360/1‑79 (MQ=255)
                                                                                                                                         cATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTAc                                                                    <  5:313360/79‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
ATTCTCGGCGATGAACTGGGGCTGTACCGGGCCATGGCCGACAGCCAGGCGGTCACCCCTGAGCAACTGGCGGAAAAGACCGGCTGCCACCCGCGGCTGGTGCGAGAATGGTTGAATGCCCAGGCGGCATCGGGCTACATGGTCCATGACAAGGGCCGTTTTGTGCTGCCTGAAGAACAGGCCATGGCCCTGGCGCTGGAGGACTCCCCGGCCTACATGGCCGGCGGCGCGGCAGTGGTGGCGGCGCTGTTCCATGACAAGGACAAGCTGGTAGCCGCCATG  >  NC_002947/3328528‑3328809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑