| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 235,390 | G→A | 8.4% | intergenic (+87/+38) | PP_0183 → / ← argH | glutathione S‑transferase/argininosuccinate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 235,390 | 0 | G | A | 8.4% | 170.3 / 3.6 | 60 | intergenic (+87/+38) | PP_0183/argH | glutathione S‑transferase/argininosuccinate lyase |
| Reads supporting (aligned to +/- strand): ref base G (30/24); new base A (3/2); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGACAAGGAAGCGTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGCGTCAGCAC > NC_002947/235244‑235538 | gCGGACAAGGAAGCGTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTAATGCACAAGTTTCAAAAACGGTGCA‑GACCTGTGGGAGCGGGCATg > 2:194410/1‑149 (MQ=17) cGGACAAGGAAGCGTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGc < 6:328956/149‑1 (MQ=255) cGGACAAGGAAGCGTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTAATGCACAAGTTTCAAAAACGGTGCA‑GACCTGTGGGAGCGGGCATGc < 1:194410/149‑1 (MQ=17) ggAAGCGTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGaa > 7:152825/1‑149 (MQ=255) aaGCGTCGATGCCGGCCTTCATGGAGATGATCGGCAGCGGCAAGGGCTGTGTGTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGTT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGTGCGGGCGTGCCCGCGAACg < 2:130921/149‑1 (MQ=38) gTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAggg > 7:261262/1‑149 (MQ=255) tGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAg < 7:179850/149‑1 (MQ=255) ttCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGccc < 2:129297/125‑1 (MQ=255) ttCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGccc > 1:129297/1‑125 (MQ=255) tgatCAGAAGCTTCAAGAGCTTAGTTTTGTGTTTACCACTACGCGTGATTGCCCTCTCCACAAGGT‑CTCCACCTTTTTTTAAATTTTTTAATTACCTGTGGTAGCGGGCTTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGAt < 4:14878/148‑1 (MQ=12) atCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAg > 7:142289/1‑149 (MQ=255) ccGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGc < 8:152825/148‑1 (MQ=255) gCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCgag > 7:238986/1‑149 (MQ=255) cGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGc < 8:275325/149‑1 (MQ=255) ggCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGcc < 8:142289/149‑1 (MQ=255) cAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCaa > 3:106790/1‑149 (MQ=255) aGCGCTGATTCTGGTGTTGAGCCCTTCGCGGTCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACa < 3:48997/149‑1 (MQ=255) aGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGcc < 4:106790/147‑1 (MQ=255) aGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCtt < 8:261262/149‑1 (MQ=255) aGTCTGGCGCTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCAACGTTTTTGAAACTTGTGTATTACCTGTGGGAGCGGGCGGGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCtt < 8:318394/149‑1 (MQ=39) tGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGGTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGCCGCCAACAGGGCCTTGGCa > 7:288208/1‑149 (MQ=255) gAGCCCTTCGCGGGCATGCCCGCACCCACAGGT‑CTGGACCCTTTTTGAAACTTGTGCATTACCTGTTGGTGCGGGCGTGCCCGCGAACGAGGCCGAAGCCCGCGCCGTGGGGGATTAGCGACACGCCAACACGGCCTTGCCgcgaacga > 4:164506/1‑149 (MQ=255) aGCCCTTCGCGGGCATGCCCGCTACCACAGGT‑ATGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGACGGCGAAGCCCACCCCTTGGGGGATTATCGAGACGCCAACAGGGCCTTGCCCCGAaagac > 2:145502/1‑149 (MQ=38) cccTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACgg > 5:220681/1‑149 (MQ=255) ttCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAgg < 8:265120/127‑1 (MQ=255) ttCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAgg > 7:265120/1‑127 (MQ=255) ggcgGGCATGCCCGCTCCCACTGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGgcagc < 1:145502/147‑1 (MQ=255) cgGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTgggg > 3:2385/1‑102 (MQ=255) GCCCGCTACCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGatg < 4:143401‑M1/99‑2 (MQ=255) cgctcccacaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGCCCTTGCCACGAACGACGGCAGCACGCACCTgcgcc > 1:183153‑M2/57‑149 (MQ=255) CTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTgcgcc > 5:1467‑M1/1‑147 (MQ=255) CTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTgcgcc < 6:1467‑M1/147‑1 (MQ=255) tcccacaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTgcgccggc > 6:3000‑M2/54‑149 (MQ=255) cacaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTggggg < 5:275434‑M2/37‑1 (MQ=255) cacaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTggggg > 6:275434‑M2/51‑87 (MQ=255) cacaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGCCCTTGCCACGAACGACGGCAGCACGCACCTgcgccggcgcc < 2:183153‑M2/99‑1 (MQ=255) cacaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcATGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTgcgccggcgcc > 3:182852‑M2/51‑149 (MQ=255) acaggtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTgcgccggcgccg > 2:85970‑M2/50‑149 (MQ=255) gtaatgcacaagtttcaaaaacggtgcattacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTac > 3:206546‑M2/47‑149 (MQ=255) gtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTac > 7:3287‑M2/46‑148 (MQ=255) gtaatgcacaagtttcaaaaacggtgcagacctgtgggagcgggcATGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTacc > 4:307138‑M2/46‑149 (MQ=255) gatGCACGGTTTTTGAAACTTGTCCATTACCTGGGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGGCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTacca < 3:164506/147‑1 (MQ=255) aCCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAAt < 6:190717/149‑1 (MQ=255) acaagtttcaaaaacggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACg > 2:357403‑M2/39‑87 (MQ=255) tGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCAc > 5:241410/1‑149 (MQ=255) aCTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCAcgcg > 6:318434/1‑148 (MQ=255) ttGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCAt > 4:54505/1‑149 (MQ=255) cggtgcagacctgtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCAtt < 5:3000‑M2/125‑1 (MQ=255) cATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCAcc < 8:238986/149‑1 (MQ=255) aTTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGGCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCAcc > 7:334818/1‑148 (MQ=255) gtgggagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTc > 4:304670‑M2/13‑149 (MQ=255) ggagcgggcATGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCa < 3:307138‑M2/139‑1 (MQ=255) gagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTcc < 6:109931‑M2/138‑1 (MQ=255) gagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTcc > 5:109931‑M2/9‑146 (MQ=255) gagcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGc > 8:46336‑M2/9‑149 (MQ=255) agcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGcc > 6:129410‑M2/8‑72 (MQ=255) agcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGcc < 5:129410‑M2/65‑1 (MQ=255) agcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGCg > 6:235221‑M2/8‑149 (MQ=255) agcgggcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGCg > 3:116305‑M2/8‑149 (MQ=255) gcgtgcGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCAACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGCGt < 5:84361‑M2/143‑1 (MQ=255) gTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGCGTCAGCAc > 6:306355/1‑149 (MQ=255) | GCGGACAAGGAAGCGTCGATGCCGGCCTTCATGGAGATGATCCGCAGCGGCAAGCGCTGAGTCTGGTGTTGAGCCCTTCGCGGGCATGCCCGCTCCCACAGGT‑CTGCACCGTTTTTGAAACTTGTGCATTACCTGTGGGAGCGGGCGTGCCCGCGAACGAGGGCGAAGCCCTCGCCTTGGGGGATTAGCGAGACGCCAACAGGGCCTTGCCACGAACGACGGCAGCACGCACCTGCGCCGGCGCCGTACCACCAATGTGGTCACGCGCATTCACCGAGCCTTCCAGCGTCAGCAC > NC_002947/235244‑235538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |