Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,333,899	A→C	6.7%	S555A (TCG→GCG)	PP_2932 ←	amidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,333,899	0	A	C	6.7%	211.8 / 3.3	71	S555A (TCG→GCG)	PP_2932	amidase family protein
Reads supporting (aligned to +/- strand):  ref base A (38/28);  new base C (3/2);  total (41/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCTGGGGGGGATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGTGACG  >  NC_002947/3333755‑3334040                                                                                                                                                 |                                                                                                                                               ttcGCTGGGGGGGATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCg                                                                                                                                            >  3:74391/1‑149 (MQ=255) ttcGCTGGGGGGGATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCg                                                                                                                                            >  7:24432/1‑149 (MQ=255)    gCTGGGGGGGATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTg                                                                                                                                         <  7:131127/149‑1 (MQ=255)             gATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCTCGCGCCggatgg                                                                                                                                       >  5:105164/1‑138 (MQ=255)             gATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCTCGCGCCggatgg                                                                                                                                       <  6:105164/142‑5 (MQ=255)                 ggCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCgg                                                                                                                            <  5:198276/149‑1 (MQ=255)                       gCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGgcagca                                                                                                                       >  2:192449/1‑148 (MQ=255)                               cAAAATCAACTGTTTGTGCATTTTTACACATATAGTTCAAATTCCCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGAGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGACTCGAACGCGGCAGCAAAACTcag                                                                                                               >  3:190256/1‑148 (MQ=255)                                  aaTCAACTGTTTGTGCATTTTTACACATATAGTTGAGATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGCGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAGCGCGGCAGCAGAGCTCagcagc                                                                                                           >  6:275444/1‑149 (MQ=255)                                   aTCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAgcg                                                                                                          >  1:266245/1‑149 (MQ=255)                                      aaCTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCTCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGt                                                                                                       >  2:36232/1‑149 (MQ=255)                                       aCTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGCCTCAGCCGAGCGCCGGCGTGCGCGGCGGAGCCCTCCGGCGCGAGCCGGTGGCCGCGAACGGGGCGGCAATCCTCAGGAGCGGGtt                                                                                                      >  4:265657/1‑149 (MQ=255)                                           tttGTGCATTTTTACACAGATAGTTGAAAGTCGCGATCTGCTGTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGGGGCCTCGACCGCGGCGGCACCTCTCAGCAGCGGGGTGTCg                                                                                                  >  4:70302/1‑149 (MQ=255)                                              gtgCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCta                                                                                               >  4:109758/1‑149 (MQ=255)                                                 cATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCTCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAg                                                                                            <  1:36232/149‑1 (MQ=255)                                                   tttttACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGc                                                                                          >  3:109652/1‑149 (MQ=255)                                                         cacaTATAGTTGAAATTCGCGATCTGCTATCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTccc                                                                                    >  5:116824/1‑149 (MQ=255)                                                            atatAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTc                                                                                                                                    <  7:187286/98‑1 (MQ=255)                                                            atatAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCgg                                                                                  >  7:99551/1‑148 (MQ=255)                                                               taGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCaaa                                                                              <  1:192449/149‑1 (MQ=255)                                                                 gTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAgg                                                                            >  4:133493/1‑149 (MQ=255)                                                                 gTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAg                                                                             <  8:99551/148‑1 (MQ=255)                                                                  ttGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCTCGCGCCggat                                                                                                                                         >  5:75529/1‑85 (MQ=255)                                                                  ttGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCTCGCGCCggat                                                                                                                                         <  6:75529/87‑3 (MQ=255)                                                                  ttGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAgg                                                                            >  7:325964/1‑148 (MQ=255)                                                                  ttGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGt                                                                           >  5:104792/1‑149 (MQ=255)                                                                   tGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAAACATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGt                                                                           >  8:325464/1‑148 (MQ=255)                                                                      aaTTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGc                                                                       <  3:174086/149‑1 (MQ=255)                                                                        ttCGCGATCTGCTTTCGCGGGCCCGGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAGCCCTCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAGAGTCAGCCGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGccc                                                                     >  2:191535/1‑149 (MQ=255)                                                                            cgATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCAcc                                                                 >  4:39322/1‑149 (MQ=255)                                                                              aTCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCgg                                                               >  5:365153/1‑149 (MQ=255)                                                                                   cTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGc                                                              <  7:325464/145‑1 (MQ=255)                                                                                   cTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATAc                                                          <  4:74391/149‑1 (MQ=255)                                                                                   cTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATAc                                                          <  4:190256/149‑1 (MQ=255)                                                                                      tCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAATGTCAGCCCCACCGGCATAcc                                                         <  3:109758/147‑1 (MQ=255)                                                                                      tCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATAcc                                                         <  8:24432/147‑1 (MQ=255)                                                                                      tCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCg                                                        <  3:133493/148‑1 (MQ=255)                                                                                        gcgGGCCCTGTAAGACTCAGCCTAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGCGCCGGTGGCCTCTAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGTCAAAGGTAAGCCCCACCGGCATACCGata                                                     >  7:54103/1‑149 (MQ=255)                                                                                        gcgGGCCCTGTAAGACTCAGCAGAGCGCCGGCGTGCGCGGCGGAACCATACGGCGCGACACGGTGGCCTCGAACGCGGCAGCAAAAATCAGCAGCGCGTTGTAGATATAGGCCTTCCCGGCAAAGGTCAGCCCCACAGGCATACCGata                                                     >  7:52399/1‑149 (MQ=255)                                                                                               cTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCg                                                                                                  >  7:173829/1‑97 (MQ=255)                                                                                               cTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCg                                                                                                  <  8:173829/97‑1 (MQ=255)                                                                                                tGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGc                                                                       >  2:255030/1‑123 (MQ=255)                                                                                                 gTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCg                                                                <  6:244627/129‑1 (MQ=255)                                                                                                 gTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCg                                                                >  5:244627/1‑129 (MQ=255)                                                                                                 gTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATc                                           >  6:344104/1‑149 (MQ=255)                                                                                                  tAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCa                                          >  8:102218/1‑149 (MQ=255)                                                                                                  tAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCa                                          >  7:249507/1‑149 (MQ=255)                                                                                                     gACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGTCAAAGGGCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGc                                       <  5:275444/149‑1 (MQ=255)                                                                                                        tCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCa                                    >  4:45287/1‑149 (MQ=255)                                                                                                        tCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCa                                    >  2:224479/1‑149 (MQ=255)                                                                                                              gAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGca                              <  3:45287/149‑1 (MQ=255)                                                                                                               aGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGcac                             <  4:109652/149‑1 (MQ=255)                                                                                                                    cGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCTGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGcacggtca                        <  2:266245/149‑1 (MQ=255)                                                                                                                        gTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGcacggtcacggt                    >  5:337397/1‑149 (MQ=255)                                                                                                                         tGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATACTCGGCCATCACGCCCATCGGcacggtcacggt                    <  1:224479/149‑1 (MQ=255)                                                                                                                           cgcgGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCgg                 <  8:325964/149‑1 (MQ=255)                                                                                                                           cgcgGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCgg                 >  8:175425/1‑149 (MQ=255)                                                                                                                                 ggAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGcc           <  5:344104/149‑1 (MQ=255)                                                                                                                                     ccATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACTGTCGGCACGCCCatg       <  8:249507/149‑3 (MQ=255)                                                                                                                                     ccATCCGGCGCGAGCCGCTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTACCGGCAAAGGTCAGCCAACCCGGCATACCGATA‑TCGGCCAACCCGCCCCTCGGCACGGTCACGGTCGGCCCGCCCAgg       >  8:185003/1‑149 (MQ=255)                                                                                                                                      cATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGt      <  7:102218/149‑1 (MQ=255)                                                                                                                                       aTCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGt      >  2:16358/1‑148 (MQ=255)                                                                                                                                        tCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGc            >  1:188991/1‑141 (MQ=255)                                                                                                                                        tCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGc            <  2:188991/141‑1 (MQ=255)                                                                                                                                        tCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGTGa    >  7:11303/1‑149 (MQ=255)                                                                                                                                          cGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCCGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGTGACg  <  7:185003/149‑1 (MQ=255)                                                                                                                                           ggCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGTGACg  >  4:10319/1‑148 (MQ=255)                                                                                                                                              gcgAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGat                                                      >  4:163910/1‑94 (MQ=255)                                                                                                                                              gcgAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGat                                                      <  3:163910/94‑1 (MQ=255)                                                                                                                                                gAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCAc                                                                  <  2:324345/80‑1 (MQ=255)                                                                                                                                                gAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCAc                                                                  >  1:324345/1‑80 (MQ=255)                                                                                                                                                 |                                                                                                                                               TTCGCTGGGGGGGATAGGCATGGCAGACCGCAAAATCAACTGTTTGTGCATTTTTACACATATAGTTGAAATTCGCGATCTGCTTTCGCGGGCCCTGTAAGACTCAGCCGAGCGCCGGCGTGCGCGGCGGAACCATCCGGCGCGAGCCGGTGGCCTCGAACGCGGCAGCAAAACTCAGCAGCGCGTTGTCGCTATAGGCCTTCCCGGCAAAGGTCAGCCCCACCGGCATACCGATA‑TCGGCCATCACGCCCATCGGCACGGTCACGGTCGGCACGCCCAGGTGACG  >  NC_002947/3333755‑3334040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑