Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,411,657	G→T	10.3%	K57N (AAG→AAT) ‡	PP_5551 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,411,657	0	G	T	10.3%	205.1 / 12.2	68	K57N (AAG→AAT) ‡	PP_5551	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/26);  new base T (3/4);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

CTTGGATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGC  >  NC_002947/3411509‑3411800                                                                                                                                                     |                                                                                                                                                cTTGGATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAg                                                                                                                                                 <  8:38841/149‑1 (MQ=255)     gATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTc                                                                                                                                              <  1:224172/148‑1 (MQ=255)     gATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTc                                                                                                                                              >  6:296572/1‑148 (MQ=255)      aTTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCaa                                                                                                                                            >  1:158771/1‑149 (MQ=255)                tCCTGTCTGTTGTAACGCTGGGCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGCtc                                                                                                                                  <  6:342574/149‑2 (MQ=255)                  ctgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGCtct                                                                                                                                 <  3:249306/148‑3 (MQ=255)                  ctgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCg                                                                                                                                >  4:142265/1‑149 (MQ=255)                   tgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGc                                                                                                                                    <  1:94748/144‑1 (MQ=255)                   tgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGc                                                                                                                                    >  2:94748/1‑144 (MQ=255)                     tctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCt                                                                                                                              <  5:296572/148‑1 (MQ=255)                       tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCtt                                                                                                                             <  8:309873/147‑1 (MQ=255)                       tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCtt                                                                                                                             >  7:309873/1‑147 (MQ=255)                       tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTg                                                                                                                            >  3:92412/1‑148 (MQ=255)                       tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTg                                                                                                                            >  6:43514/1‑148 (MQ=255)                            tAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGaa                                                                                                                         >  2:259840/1‑146 (MQ=255)                              aCGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                     <  7:283718/148‑1 (MQ=255)                               cGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGAtt                                                                                                                   <  5:276229/149‑1 (MQ=255)                                       gCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTc                                                                                                               >  3:139843/1‑145 (MQ=255)                                        cTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGa                                                                                                                          >  5:236680/1‑133 (MQ=255)                                        cTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGa                                                                                                                          <  6:236680/133‑1 (MQ=255)                                          cAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAtt                                                                                                         <  4:92412/148‑1 (MQ=255)                                             aaTAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAg                                                                                                                      <  4:91579/132‑1 (MQ=255)                                             aaTAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAg                                                                                                                      >  3:91579/1‑132 (MQ=255)                                                      agcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                     >  5:243795/1‑124 (MQ=255)                                                      agcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                     <  6:243795/124‑1 (MQ=255)                                                       gcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAAATCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTg                                                                                           >  5:328877/1‑149 (MQ=255)                                                        caagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTgg                                                                                          <  3:142265/149‑1 (MQ=255)                                                           gcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTg                                                                                       >  1:133067/1‑149 (MQ=255)                                                              aTCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCa                                                                                    <  6:328877/149‑1 (MQ=255)                                                                  gCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAttt                                                                                                        >  3:215727/1‑125 (MQ=255)                                                                  gCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAttt                                                                                                        <  4:215727/125‑1 (MQ=255)                                                                   cGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCtt                                                                               <  5:43514/149‑1 (MQ=255)                                                                    ggCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCtt                                                                               >  2:191070/1‑148 (MQ=255)                                                                    ggCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTc                                                                              <  3:231367/149‑1 (MQ=255)                                                                                 aaTTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAg                                                                 >  7:157049/1‑149 (MQ=255)                                                                                  aTTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGt                                                                <  1:191070/149‑1 (MQ=255)                                                                                     tAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGa                                                              <  5:191252/148‑1 (MQ=255)                                                                                           gTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGt                                                       >  4:58374/1‑149 (MQ=255)                                                                                            tCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGt                                                       >  5:64072/1‑148 (MQ=255)                                                                                              aGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAg                                                    >  7:169989/1‑149 (MQ=255)                                                                                                   gcgAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGgcgc                                               >  2:153147/1‑149 (MQ=255)                                                                                                     gAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTa                                             >  5:22536/1‑149 (MQ=255)                                                                                                        ggATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACtt                                          <  3:58374/149‑1 (MQ=255)                                                                                                        ggATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACt                                           <  2:158771/148‑1 (MQ=255)                                                                                                         gATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTc                                         >  4:39697/1‑149 (MQ=255)                                                                                                           tCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCg                                       >  6:220273/1‑149 (MQ=255)                                                                                                              tGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCt                                    >  2:338365/1‑149 (MQ=255)                                                                                                               gAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCtt                                   >  4:46918/1‑149 (MQ=255)                                                                                                                aaGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCtt                                   <  6:22536/148‑1 (MQ=255)                                                                                                                  gATCTAATGAATTCTCCAAAAGAAGAGCTTGCGAAGGTCATTCGAAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACTCGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGAt                                <  6:64072/149‑1 (MQ=255)                                                                                                                      tAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGc                                      >  4:301526/1‑139 (MQ=255)                                                                                                                      tAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGc                                      <  3:301526/139‑1 (MQ=255)                                                                                                                         aGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         <  1:153147/149‑1 (MQ=255)                                                                                                                         aGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         <  5:220273/149‑1 (MQ=255)                                                                                                                            aTTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGt                       >  6:318241/1‑148 (MQ=255)                                                                                                                                tGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAgg                  >  7:20497/1‑149 (MQ=255)                                                                                                                                tGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAgg                  >  8:302896/1‑149 (MQ=255)                                                                                                                                   aaaaGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGc                              <  6:362398/134‑1 (MQ=255)                                                                                                                                    caagcgaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTa                >  4:173456/8‑147 (MQ=255)                                                                                                                                     aagaagAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         >  2:261069/1‑137 (MQ=255)                                                                                                                                     aagaagAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         <  1:261069/137‑1 (MQ=255)                                                                                                                                       gaagaGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGa           >  2:109180/1‑149 (MQ=255)                                                                                                                                         gaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAg                   <  3:143369/137‑1 (MQ=255)                                                                                                                                         gaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAg                   >  4:143369/3‑139 (MQ=255)                                                                                                                                             cTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTc     >  6:32525/1‑149 (MQ=255)                                                                                                                                             cTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTc     >  2:168218/1‑149 (MQ=255)                                                                                                                                              ttGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCg    >  6:140385/1‑149 (MQ=255)                                                                                                                                                gCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCg    <  8:169989/147‑1 (MQ=255)                                                                                                                                                  gAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGc  >  2:315836/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                CTTGGATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGC  >  NC_002947/3411509‑3411800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑