Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,411,659 T→A 10.2% V58D (GTC→GAC)  PP_5551 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,411,6590TA10.2% 180.2 / 8.4 68V58D (GTC→GAC) PP_5551hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/26);  new base A (3/4);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGC  >  NC_002947/3411513‑3411800
                                                                                                                                                  |                                                                                                                                             
gATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTc                                                                                                                                              <  1:224172/148‑1 (MQ=255)
gATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTc                                                                                                                                              >  6:296572/1‑148 (MQ=255)
 aTTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCaa                                                                                                                                            >  1:158771/1‑149 (MQ=255)
           tCCTGTCTGTTGTAACGCTGGGCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGCtc                                                                                                                                  <  6:342574/149‑2 (MQ=255)
             ctgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGCtct                                                                                                                                 <  3:249306/148‑3 (MQ=255)
             ctgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCg                                                                                                                                >  4:142265/1‑149 (MQ=255)
              tgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGc                                                                                                                                    >  2:94748/1‑144 (MQ=255)
              tgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGc                                                                                                                                    <  1:94748/144‑1 (MQ=255)
                tctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCt                                                                                                                              <  5:296572/148‑1 (MQ=255)
                  tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCtt                                                                                                                             <  8:309873/147‑1 (MQ=255)
                  tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCtt                                                                                                                             >  7:309873/1‑147 (MQ=255)
                  tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTg                                                                                                                            >  6:43514/1‑148 (MQ=255)
                  tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTg                                                                                                                            >  3:92412/1‑148 (MQ=255)
                       tAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGaa                                                                                                                         >  2:259840/1‑146 (MQ=255)
                         aCGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                     <  7:283718/148‑1 (MQ=255)
                          cGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGAtt                                                                                                                   <  5:276229/149‑1 (MQ=255)
                                  gCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTc                                                                                                               >  3:139843/1‑145 (MQ=255)
                                   cTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGa                                                                                                                          >  5:236680/1‑133 (MQ=255)
                                   cTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGa                                                                                                                          <  6:236680/133‑1 (MQ=255)
                                     cAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAtt                                                                                                         <  4:92412/148‑1 (MQ=255)
                                        aaTAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAg                                                                                                                      >  3:91579/1‑132 (MQ=255)
                                        aaTAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAg                                                                                                                      <  4:91579/132‑1 (MQ=255)
                                                 agcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                     <  6:243795/124‑1 (MQ=255)
                                                 agcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                     >  5:243795/1‑124 (MQ=255)
                                                  gcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAAATCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTg                                                                                           >  5:328877/1‑149 (MQ=255)
                                                   caagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTgg                                                                                          <  3:142265/149‑1 (MQ=255)
                                                      gcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTg                                                                                       >  1:133067/1‑149 (MQ=255)
                                                         aTCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCa                                                                                    <  6:328877/149‑1 (MQ=255)
                                                             gCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAttt                                                                                                        >  3:215727/1‑125 (MQ=255)
                                                             gCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAttt                                                                                                        <  4:215727/125‑1 (MQ=255)
                                                              cGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCtt                                                                               <  5:43514/149‑1 (MQ=255)
                                                               ggCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCtt                                                                               >  2:191070/1‑148 (MQ=255)
                                                               ggCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTc                                                                              <  3:231367/149‑1 (MQ=255)
                                                                            aaTTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAg                                                                 >  7:157049/1‑149 (MQ=255)
                                                                             aTTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGt                                                                <  1:191070/149‑1 (MQ=255)
                                                                                tAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGa                                                              <  5:191252/148‑1 (MQ=255)
                                                                                      gTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGt                                                       >  4:58374/1‑149 (MQ=255)
                                                                                       tCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGt                                                       >  5:64072/1‑148 (MQ=255)
                                                                                         aGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAg                                                    >  7:169989/1‑149 (MQ=255)
                                                                                              gcgAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGgcgc                                               >  2:153147/1‑149 (MQ=255)
                                                                                                gAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTa                                             >  5:22536/1‑149 (MQ=255)
                                                                                                   ggATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACtt                                          <  3:58374/149‑1 (MQ=255)
                                                                                                   ggATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACt                                           <  2:158771/148‑1 (MQ=255)
                                                                                                    gATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTc                                         >  4:39697/1‑149 (MQ=255)
                                                                                                      tCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCg                                       >  6:220273/1‑149 (MQ=255)
                                                                                                         tGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCt                                    >  2:338365/1‑149 (MQ=255)
                                                                                                          gAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCtt                                   >  4:46918/1‑149 (MQ=255)
                                                                                                           aaGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCtt                                   <  6:22536/148‑1 (MQ=255)
                                                                                                             gATCTAATGAATTCTCCAAAAGAAGAGCTTGCGAAGGTCATTCGAAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACTCGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGAt                                <  6:64072/149‑1 (MQ=255)
                                                                                                                 tAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGc                                      >  4:301526/1‑139 (MQ=255)
                                                                                                                 tAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGc                                      <  3:301526/139‑1 (MQ=255)
                                                                                                                    aGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         <  5:220273/149‑1 (MQ=255)
                                                                                                                    aGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         <  1:153147/149‑1 (MQ=255)
                                                                                                                       aTTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGt                       >  6:318241/1‑148 (MQ=255)
                                                                                                                           tGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAgg                  >  7:20497/1‑149 (MQ=255)
                                                                                                                           tGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAgg                  >  8:302896/1‑149 (MQ=255)
                                                                                                                              aaaaGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGc                              <  6:362398/134‑1 (MQ=255)
                                                                                                                               caagcgaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTa                >  4:173456/8‑147 (MQ=255)
                                                                                                                                aagaagAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         >  2:261069/1‑137 (MQ=255)
                                                                                                                                aagaagAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                         <  1:261069/137‑1 (MQ=255)
                                                                                                                                  gaagaGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGa           >  2:109180/1‑149 (MQ=255)
                                                                                                                                    gaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAg                   <  3:143369/137‑1 (MQ=255)
                                                                                                                                    gaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAg                   >  4:143369/3‑139 (MQ=255)
                                                                                                                                        cTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTc     >  6:32525/1‑149 (MQ=255)
                                                                                                                                        cTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTc     >  2:168218/1‑149 (MQ=255)
                                                                                                                                         ttGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCg    >  6:140385/1‑149 (MQ=255)
                                                                                                                                           gCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCg    <  8:169989/147‑1 (MQ=255)
                                                                                                                                             gAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGc  >  2:315836/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGC  >  NC_002947/3411513‑3411800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑