Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,411,662 A→T 12.0% N59I (AAT→ATT) ‡ PP_5551 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,411,6620AT12.0% 167.9 / 11.6 65N59I (AAT→ATT) ‡PP_5551hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/24);  new base T (3/5);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGCAAGATGTCTT  >  NC_002947/3411514‑3411810
                                                                                                                                                    |                                                                                                                                                    
aTTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCaa                                                                                                                                                      >  1:158771/1‑149 (MQ=255)
          tCCTGTCTGTTGTAACGCTGGGCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGCtc                                                                                                                                            <  6:342574/149‑2 (MQ=255)
            ctgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGCtct                                                                                                                                           <  3:249306/148‑3 (MQ=255)
            ctgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCg                                                                                                                                          >  4:142265/1‑149 (MQ=255)
             tgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGc                                                                                                                                              >  2:94748/1‑144 (MQ=255)
             tgtctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGATTGACCTTCGCAAGc                                                                                                                                              <  1:94748/144‑1 (MQ=255)
               tctgtTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCt                                                                                                                                        <  5:296572/148‑1 (MQ=255)
                 tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCtt                                                                                                                                       >  7:309873/1‑147 (MQ=255)
                 tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCtt                                                                                                                                       <  8:309873/147‑1 (MQ=255)
                 tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTg                                                                                                                                      >  6:43514/1‑148 (MQ=255)
                 tgttgtAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTg                                                                                                                                      >  3:92412/1‑148 (MQ=255)
                      tAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGaa                                                                                                                                   >  2:259840/1‑146 (MQ=255)
                        aCGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                               <  7:283718/148‑1 (MQ=255)
                         cGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGAtt                                                                                                                             <  5:276229/149‑1 (MQ=255)
                                 gCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTc                                                                                                                         >  3:139843/1‑145 (MQ=255)
                                  cTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGa                                                                                                                                    <  6:236680/133‑1 (MQ=255)
                                  cTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGa                                                                                                                                    >  5:236680/1‑133 (MQ=255)
                                    cAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAtt                                                                                                                   <  4:92412/148‑1 (MQ=255)
                                       aaTAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAg                                                                                                                                >  3:91579/1‑132 (MQ=255)
                                       aaTAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAg                                                                                                                                <  4:91579/132‑1 (MQ=255)
                                                agcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                               <  6:243795/124‑1 (MQ=255)
                                                agcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGa                                                                                                                               >  5:243795/1‑124 (MQ=255)
                                                 gcaagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAAATCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTg                                                                                                     >  5:328877/1‑149 (MQ=255)
                                                  caagcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTgg                                                                                                    <  3:142265/149‑1 (MQ=255)
                                                     gcGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTg                                                                                                 >  1:133067/1‑149 (MQ=255)
                                                        aTCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCa                                                                                              <  6:328877/149‑1 (MQ=255)
                                                            gCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAttt                                                                                                                  >  3:215727/1‑125 (MQ=255)
                                                            gCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGAttt                                                                                                                  <  4:215727/125‑1 (MQ=255)
                                                             cGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCtt                                                                                         <  5:43514/149‑1 (MQ=255)
                                                              ggCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCtt                                                                                         >  2:191070/1‑148 (MQ=255)
                                                              ggCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTc                                                                                        <  3:231367/149‑1 (MQ=255)
                                                                           aaTTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAg                                                                           >  7:157049/1‑149 (MQ=255)
                                                                            aTTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGt                                                                          <  1:191070/149‑1 (MQ=255)
                                                                               tAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGa                                                                        <  5:191252/148‑1 (MQ=255)
                                                                                     gTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGt                                                                 >  4:58374/1‑149 (MQ=255)
                                                                                      tCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGt                                                                 >  5:64072/1‑148 (MQ=255)
                                                                                        aGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAg                                                              >  7:169989/1‑149 (MQ=255)
                                                                                             gcgAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGgcgc                                                         >  2:153147/1‑149 (MQ=255)
                                                                                               gAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTa                                                       >  5:22536/1‑149 (MQ=255)
                                                                                                  ggATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACtt                                                    <  3:58374/149‑1 (MQ=255)
                                                                                                  ggATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACt                                                     <  2:158771/148‑1 (MQ=255)
                                                                                                   gATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTc                                                   >  4:39697/1‑149 (MQ=255)
                                                                                                     tCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCg                                                 >  6:220273/1‑149 (MQ=255)
                                                                                                        tGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCt                                              >  2:338365/1‑149 (MQ=255)
                                                                                                         gAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCtt                                             >  4:46918/1‑149 (MQ=255)
                                                                                                          aaGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCtt                                             <  6:22536/148‑1 (MQ=255)
                                                                                                            gATCTAATGAATTCTCCAAAAGAAGAGCTTGCGAAGGTCATTCGAAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACTCGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGAt                                          <  6:64072/149‑1 (MQ=255)
                                                                                                                tAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGc                                                <  3:301526/139‑1 (MQ=255)
                                                                                                                tAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGc                                                >  4:301526/1‑139 (MQ=255)
                                                                                                                   aGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                                   <  1:153147/149‑1 (MQ=255)
                                                                                                                   aGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                                   <  5:220273/149‑1 (MQ=255)
                                                                                                                      aTTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGt                                 >  6:318241/1‑148 (MQ=255)
                                                                                                                          tGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAgg                            >  8:302896/1‑149 (MQ=255)
                                                                                                                          tGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAgg                            >  7:20497/1‑149 (MQ=255)
                                                                                                                             aaaaGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGc                                        <  6:362398/134‑1 (MQ=255)
                                                                                                                              caagcgaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTa                          >  4:173456/8‑147 (MQ=255)
                                                                                                                               aagaagAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                                   <  1:261069/137‑1 (MQ=255)
                                                                                                                               aagaagAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTaaaa                                   >  2:261069/1‑137 (MQ=255)
                                                                                                                                 gaagaGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGa                     >  2:109180/1‑149 (MQ=255)
                                                                                                                                   gaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAg                             <  3:143369/137‑1 (MQ=255)
                                                                                                                                   gaagCTTGCGATTGACCTTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAg                             >  4:143369/3‑139 (MQ=255)
                                                                                                                                       cTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTc               >  2:168218/1‑149 (MQ=255)
                                                                                                                                       cTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTc               >  6:32525/1‑149 (MQ=255)
                                                                                                                                        ttGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCg              >  6:140385/1‑149 (MQ=255)
                                                                                                                                          gCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCg              <  8:169989/147‑1 (MQ=255)
                                                                                                                                            gAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGc            >  2:315836/1‑147 (MQ=255)
                                                                                                                                                   aaTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGa                                                                               >  6:40079/1‑73 (MQ=255)
                                                                                                                                                   aaTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGa                                                                               <  5:40079/73‑1 (MQ=255)
                                                                                                                                                   aaTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGCAAGATGTCt   >  7:238600/1‑149 (MQ=255)
                                                                                                                                                    aTCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGCAAGATGTCtt  <  8:157049/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTGCACTTGTCCTGTCTGTTGTAACGCTGGTCGCTCAGAATAAGCAAAGCAAGCGATCTGCGGCTGATCAGGCTAATTTAGCAAGTCAGGCCGCGAGGGATCGTGAAGATCTAAAGAATTCTGCAAAAGAAGAGCTTGCGAAGGTCAATCGCAAGCTTCGCTTGGAAAAGATTACTCAAGATTTAGACGAGCTTTTGGCTGTCACTCTTCAGTATTGGACGAGTGACGGCCGTGAGGGCGCTACTTCCGCCTTGATGCTAAAAGTCAAGGTAAAGGACCTTTCGGCAAGATGTCTT  >  NC_002947/3411514‑3411810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑