Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,483,798 G→C 6.5% P709R (CCG→CGG) ‡ PP_3091 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,483,7980GC 6.5% 231.8 / 4.3 73P709R (CCG→CGG) ‡PP_3091membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (3/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GGACCAGGTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAGGCCGGGTCTG  >  NC_002947/3483655‑3483942
                                                                                                                                               |                                                                                                                                                
ggACCAGGTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTTGTTGATCAGCTGTTCGTTGCGCGGCAac                                                                                                                                             <  4:8502/149‑1 (MQ=255)
     aGGTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACgg                                                                                                                                        >  5:152806/1‑149 (MQ=255)
     aGGTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCCGCGCAacgaaca                                                                                                                                        <  3:182382/149‑4 (MQ=255)
      ggTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGgc                                                                                                                                       <  6:19004/149‑1 (MQ=255)
      ggTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGgc                                                                                                                                       >  8:185103/1‑149 (MQ=255)
              aGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGcc                                                                                                                               <  2:160421/149‑1 (MQ=255)
                  tCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGc                                                                                                                           >  2:110808/1‑149 (MQ=255)
                  tCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGc                                                                                                                           >  2:106596/1‑149 (MQ=255)
                    aGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGc                                                                                                                           <  3:59112/147‑1 (MQ=255)
                                      ctcgactcgaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAg                                                                                                       >  3:259292/1‑149 (MQ=255)
                                       tcgactcgaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACt                                                                                                                 >  4:179106/1‑138 (MQ=255)
                                       tcgactcgaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACt                                                                                                                 <  3:179106/138‑1 (MQ=255)
                                          actcgaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCggg                                                                                                   >  5:251501/1‑149 (MQ=255)
                                           ctcgaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCgggg                                                                                                  >  6:82140/1‑149 (MQ=255)
                                            tcgaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGTGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCgggg                                                                                                  >  8:149752/1‑148 (MQ=255)
                                              gaGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGc                                                                                                 <  5:82140/147‑1 (MQ=255)
                                               aGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCAtt                                                                                              >  4:128758/1‑149 (MQ=255)
                                                       gcgcAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGa                                                                                      <  7:66862/149‑1 (MQ=255)
                                                           aGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCaa                                                                                                                   <  3:131184/116‑1 (MQ=255)
                                                           aGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCaa                                                                                                                   >  4:131184/1‑116 (MQ=255)
                                                              tATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGc                                                                                >  3:345513/1‑148 (MQ=255)
                                                                   gcgATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGt                                                                          >  6:25364/1‑149 (MQ=255)
                                                                   gcgATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGt                                                                          >  6:2197/1‑149 (MQ=255)
                                                                   gcgATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGACGGGTTTCGTTGATCAGCTGTTCGTTGCGCGCCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGt                                                                          >  5:110479/1‑149 (MQ=255)
                                                                      aTCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGgc                                                                                                                                       >  8:291958/1‑85 (MQ=255)
                                                                      aTCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGgc                                                                                                                                       <  7:291958/85‑1 (MQ=255)
                                                                      aTCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCgg                                                                       >  5:49229/1‑149 (MQ=255)
                                                                         ctccAGCTCGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCg                                                                    >  1:14631/3‑149 (MQ=255)
                                                                           ccAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCggg                                                                  <  5:2197/149‑1 (MQ=255)
                                                                             aGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGt                                                                <  5:25364/149‑1 (MQ=255)
                                                                                gAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGcc                                                                               <  3:70591/131‑1 (MQ=255)
                                                                                gAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGcc                                                                               >  4:70591/1‑131 (MQ=255)
                                                                                     ggAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGc                                                        <  7:185103/149‑1 (MQ=255)
                                                                                      gagaTCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGcc                                                       >  7:153280/1‑149 (MQ=255)
                                                                                            aTGAACGATTTGAGTTTCTGCCGGTTTTCGTTGTTCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGc                                                                                        <  2:267160/110‑1 (MQ=255)
                                                                                            aTGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGc                                                                                        >  1:267160/1‑110 (MQ=255)
                                                                                            aTGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTgcggc                                                 >  7:49423/1‑149 (MQ=255)
                                                                                            aTGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTgcggc                                                 >  4:156694/1‑149 (MQ=255)
                                                                                            aTGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTgcgg                                                  <  6:49229/148‑1 (MQ=255)
                                                                                             tGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGc                                                                                                               <  2:261861/86‑1 (MQ=255)
                                                                                             tGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGc                                                                                                               >  1:261861/1‑86 (MQ=255)
                                                                                                aCGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTgc                                                    >  5:117214/1‑142 (MQ=255)
                                                                                                aCGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTgc                                                    <  6:117214/142‑1 (MQ=255)
                                                                                                  gACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGc                                           <  6:152806/149‑1 (MQ=255)
                                                                                                    cTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAATGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCCCTGGcc                                          >  2:351359/1‑148 (MQ=255)
                                                                                                     ttGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCCGCAACGACGGCGCCTGGCACTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGcc                                          >  5:252550/1‑147 (MQ=255)
                                                                                                          gTTCTGCCGGGTTTCGTGGATCAGCTGTTCGTTGCGCGGCAACGCCGGCGCCTGGCCCTGCTCCCGCAACTGCACGTACCGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCt                                   >  6:274808/1‑149 (MQ=255)
                                                                                                             cTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGACTTCGACTGCGGCGCTGGCCCAGGCCAggg                                >  6:189874/1‑149 (MQ=255)
                                                                                                              tGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTgggg                               >  6:265577/1‑149 (MQ=255)
                                                                                                               gCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCgg                                                                       <  5:9885/108‑1 (MQ=255)
                                                                                                               gCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCgg                                                                       >  6:9885/1‑108 (MQ=255)
                                                                                                               gCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGc                              >  8:301909/1‑149 (MQ=255)
                                                                                                                ccGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCCGGCCTgggg                               >  2:341095/1‑147 (MQ=255)
                                                                                                                ccGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTgggg                               <  3:128758/147‑1 (MQ=255)
                                                                                                                ccGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTggg                                <  1:110808/146‑1 (MQ=255)
                                                                                                                ccGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTggg                                <  1:106596/146‑1 (MQ=255)
                                                                                                                ccGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCAGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTgggg                               >  2:41836/1‑147 (MQ=255)
                                                                                                                 cGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAg                            <  8:153280/149‑1 (MQ=255)
                                                                                                                 agggTTTCGTTGAGCTGCTGTTCGTTGCGCGGCATCGACGTCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCGAGCGTCGGCCTGGGTGCGTTCGCCTGCGGCGCTGGCCCAGGGCTGGGGCAg                            <  4:29509/148‑1 (MQ=255)
                                                                                                                     tttCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTggg                                >  7:92361/1‑141 (MQ=255)
                                                                                                                     tttCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTggg                                <  8:92361/141‑1 (MQ=255)
                                                                                                                       tCGGTGATCAGCTGTTGGTTGAGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTgg                       <  6:251501/148‑1 (MQ=255)
                                                                                                                         gTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCa                     <  4:259292/148‑1 (MQ=255)
                                                                                                                         gTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCa                     <  8:49423/148‑1 (MQ=255)
                                                                                                                          ttGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGc                   <  4:345513/149‑1 (MQ=255)
                                                                                                                             aTCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGcga                 <  2:14631/148‑1 (MQ=255)
                                                                                                                                  cTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAgg           >  8:76531/1‑149 (MQ=255)
                                                                                                                                    cgtCGTTGCCGCGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCg                                                         >  6:273847/3‑101 (MQ=255)
                                                                                                                                    cgtCGTTGCCGCGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCg                                                         <  5:273847/99‑1 (MQ=255)
                                                                                                                                    cgtCGTTGCCGCGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAGGcc         >  2:205368/3‑149 (MQ=255)
                                                                                                                                     ttCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAGGc          <  3:156694/147‑1 (MQ=255)
                                                                                                                                     ttCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAGGc          <  6:110479/147‑1 (MQ=255)
                                                                                                                                           gcgcGGCAACGACGGCGCCTAGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAGGCCGGGTCTg  >  4:298829/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGACCAGGTCGTTGAGGCTCAGCGCCGGGAACTGCCGGCTCGACTCGAGCTGCAGGCGCAGGTATTCGCGATCGACCAGCGAGCTGGAGATCATGAACGACTTGAGGTTCTGCCGGGTTTCGTTGATCAGCTGTTCGTTGCGCGGCAACGACGGCGCCTGGCCCTGCTCCAGCAACTGCACGTACAGCGGGGCATTTTCTGCGATCACGCCAGCGTCGGCCGGGGTGCCTTCGCCTGCGGCGCTGGCCCAGGCCTGGGGCAGGCTGGCAGCGACGAAGGCCGGGTCTG  >  NC_002947/3483655‑3483942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑