Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,556,084 A→C 8.8% A167A (GCA→GCC)  PP_3140 → group 2 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,556,0840AC 8.8% 190.3 / 7.4 67A167A (GCA→GCC) PP_3140group 2 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base C (2/4);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CGCCACCTGGCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGAGTCTGATCAAC  >  NC_002947/3555938‑3556231
                                                                                                                                                  |                                                                                                                                                   
cgcCACCTGGCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCTGCCgg                                                                                                                                                   <  1:319539/149‑1 (MQ=255)
 gcCACCTGGCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGt                                                                                                                                                  >  2:62258/1‑149 (MQ=255)
      cTGGCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTgg                                                                                                                                             >  3:64030/1‑149 (MQ=255)
      cTGGCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTgg                                                                                                                                             >  3:59182/1‑149 (MQ=255)
         gCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCTGCCGGTGcacgcat                                                                                                                                          <  7:70640/149‑7 (MQ=255)
            cAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAg                                                                                                                                       >  8:134889/1‑149 (MQ=255)
             aGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAgg                                                                                                                                      <  4:83484/149‑1 (MQ=255)
                    gccCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAgg                                                                                                                               >  2:324504/1‑149 (MQ=255)
                      ccGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGtt                                                                                                                             <  8:93001/149‑1 (MQ=255)
                        gatgtggtCATCGTGGTGGATGCCGACTGCCAGGTGGTCGAGGGGTCTATCGACTGCCTGGCGCGCCGCTGCCACGAACTTGCCCTCCAGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGt                                                                                                                                                  <  1:220273/123‑1 (MQ=255)
                        gAGGTGGTCATCGTGGTGGATGTCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGc                                                                                                                           >  7:53102/1‑149 (MQ=255)
                              gtCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGtt                                                                                                                     >  7:139219/1‑149 (MQ=255)
                               tCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGttt                                                                                                                    >  5:302390/1‑149 (MQ=255)
                                  tCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGcc                                                                                                                 <  6:188334/149‑1 (MQ=255)
                                      ggtggATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGc                                                                                                             <  4:229610/149‑1 (MQ=255)
                                       gtggATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGc                                                                                                             <  7:311634/148‑1 (MQ=255)
                                        tggATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCgg                                                                                                           <  8:53102/149‑1 (MQ=255)
                                              ccGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCaa                                                                                                      <  4:59182/148‑1 (MQ=255)
                                              ccGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCaa                                                                                                      <  4:64030/148‑1 (MQ=255)
                                              ccGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCaa                                                                                                      <  6:302390/148‑1 (MQ=255)
                                                gACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGaa                                                                                                   >  8:321088/1‑149 (MQ=255)
                                                gACGGCCAGGGGGGCGAGTGGGCGAGCGACTGCCGGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGGACTGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGaa                                                                                                   <  5:4863/149‑1 (MQ=255)
                                                 aCTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAAc                                                                                                  <  3:217810/149‑1 (MQ=255)
                                                     ccAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAg                                                                                                                       <  1:24219/124‑1 (MQ=255)
                                                     ccAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAg                                                                                                                       >  2:24219/1‑124 (MQ=255)
                                                     ccAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTg                                                                                               <  8:139219/148‑1 (MQ=255)
                                                             gCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCa                                                                                      >  6:205576/1‑149 (MQ=255)
                                                             gCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCa                                                                                      >  3:119909/1‑149 (MQ=255)
                                                                    ggCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTg                                                                                               >  6:189309/1‑133 (MQ=255)
                                                                    ggCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTg                                                                                               <  5:189309/133‑1 (MQ=255)
                                                                     gCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGc                                                                                                                                  >  1:151583/1‑97 (MQ=255)
                                                                     gCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGc                                                                                                                                  <  2:151583/97‑1 (MQ=255)
                                                                      cGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTgg                                                                             <  7:134889/149‑1 (MQ=255)
                                                                       gATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGt                                                                                                                      >  8:293750/1‑107 (MQ=255)
                                                                       gATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGt                                                                                                                      <  7:293750/107‑1 (MQ=255)
                                                                           gACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGgcgc                                                                         <  6:350640/148‑1 (MQ=255)
                                                                            aCTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGgcgcgc                                                                       >  6:251495/1‑149 (MQ=255)
                                                                                      gcgcCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCtgc                                                             >  3:187053/1‑149 (MQ=255)
                                                                                         ccgcTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCtgccat                                                          <  1:62258/149‑1 (MQ=255)
                                                                                             tGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCtgccatgcc                                                       >  2:290767/1‑148 (MQ=255)
                                                                                              gCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCaa                                                     <  4:119909/149‑1 (MQ=255)
                                                                                                 aCGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTgg                                                                   >  4:8593/1‑132 (MQ=255)
                                                                                                 aCGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTgg                                                                   <  3:8593/132‑1 (MQ=255)
                                                                                                   gCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGAt                                                <  4:187053/149‑1 (MQ=255)
                                                                                                       ttGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGgc                                            >  6:108378/1‑149 (MQ=255)
                                                                                                            cgcccgGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGATTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCggg                                       >  3:29476/1‑149 (MQ=255)
                                                                                                               ccgGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCAt                                    >  4:73295/1‑149 (MQ=255)
                                                                                                                cgGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATg                                   <  3:73295/149‑1 (MQ=255)
                                                                                                                 ggTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGGTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCATGGGCCTGCCATGCCAACTGATGGGCGCGGGCATgg                                  >  2:181781/1‑149 (MQ=255)
                                                                                                                  gTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGc                                 >  4:69026/1‑149 (MQ=255)
                                                                                                                      aGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTc                             >  7:255048/1‑149 (MQ=255)
                                                                                                                          gCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCt                         <  1:324504/149‑1 (MQ=255)
                                                                                                                            tGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTgg                       >  5:364793/1‑149 (MQ=255)
                                                                                                                                  tGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTgg                                                                   <  4:56569/99‑1 (MQ=255)
                                                                                                                                  tGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTgg                                                                   >  3:56569/1‑99 (MQ=255)
                                                                                                                                   gATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATg                                   <  6:302468/130‑1 (MQ=255)
                                                                                                                                   gATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATg                                   >  5:302468/1‑130 (MQ=255)
                                                                                                                                   gATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATc                >  5:6975/1‑149 (MQ=255)
                                                                                                                                    aTGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGAt                 <  3:69026/147‑1 (MQ=255)
                                                                                                                                      cccagCACCTGCCGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCt               >  4:291083/5‑147 (MQ=255)
                                                                                                                                      cccagCACCTGCCGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGa             <  3:296096/145‑1 (MQ=255)
                                                                                                                                      cccagCACCTGCCGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGa             >  4:296096/5‑149 (MQ=255)
                                                                                                                                      cccagCACCTGCCGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGa             <  3:291083/145‑1 (MQ=255)
                                                                                                                                           cACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCa                     >  4:250548/1‑136 (MQ=255)
                                                                                                                                           cACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCa                     <  3:250548/136‑1 (MQ=255)
                                                                                                                                             ccGGCAGGTGGTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGAGTCTGAt      <  8:255048/149‑1 (MQ=255)
                                                                                                                                                 cAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGAGTCTGATCAAc  <  6:6975/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCCACCTGGCTCAGCGGCCGCCCGAGGTGGTCATCGTGGTGGATGCCGACTGCCAGGTGGGCGAGGGGGCGATCGACTGCCTGGCGCGCCGCTGCCACGCACTTGCCCGCCCGGTACAGTCGCTGTATCTGATGCGTGCACCGGCAGGTGCTGGGTTGAGGGTGCAGGTTGCCGAGTTTGCCTGGCGGGTCAAGAACCTGGTGCGGCCACGTGGCTGGGCGCGCCTGGGCCTGCCATGCCAACTGATGGGCGCGGGCATGGCGTTCGGCTGGCACGATCTGAGTCTGATCAAC  >  NC_002947/3555938‑3556231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑