Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,563,387 T→C 7.8% D146G (GAC→GGC)  PP_3146 ← oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,563,3870TC 7.8% 174.0 / 3.6 64D146G (GAC→GGC) PP_3146oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base C (2/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCAGGTAGC  >  NC_002947/3563240‑3563534
                                                                                                                                                   |                                                                                                                                                   
gCTTCGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgt                                                                                                                                                     >  6:121918/1‑148 (MQ=255)
gCTTCGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgt                                                                                                                                                     <  5:121918/148‑1 (MQ=255)
  ttCGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgtcgt                                                                                                                                                  >  7:41329/1‑149 (MQ=255)
    cGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgtcgtc                                                                                                                                                 <  2:327579/148‑1 (MQ=255)
    cGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgtcgtc                                                                                                                                                 <  6:130820/148‑1 (MQ=255)
      cccTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTg                                                                                                                                              >  7:221496/1‑149 (MQ=255)
        cTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGtt                                                                                                                                            >  8:200116/1‑149 (MQ=255)
               aGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCtt                                                                                                                                     <  2:103202/149‑1 (MQ=255)
                gATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTg                                                                                                                                    <  3:301603/149‑1 (MQ=255)
                  tCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTg                                                                                                                                    >  2:280117/1‑147 (MQ=255)
                  tCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGaa                                                                                                                                  <  7:200116/149‑1 (MQ=255)
                                gTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgtcgtc                                                                                                                                                 >  3:290132/1‑120 (MQ=255)
                                gTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGgtcgtc                                                                                                                                                 <  4:290132/120‑1 (MQ=255)
                                   gCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCTGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGACGACCTGGCCGCCCTgg                                                                                                                                             <  5:76550/121‑2 (MQ=255)
                                   gCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGACGACCTGGCCGCCCTgg                                                                                                                                             >  6:76550/1‑120 (MQ=255)
                                    cccGAGCCCATATCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGcgccgc                                                                                                                >  8:165485/1‑149 (MQ=255)
                                     ccGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGCCCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTGCTCCTTGAAGGCGGCGAACTGCGCcgcg                                                                                                               >  5:198718/1‑149 (MQ=255)
                                     ccGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGAGCcgcg                                                                                                               >  2:244650/1‑149 (MQ=255)
                                     ccGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGGGTTTCATGGCGCTAGCCCAGGGCGGCCAGGGCGTCCTGTTCCTCCTTGAAggcgg                                                                                                                             >  8:75290/1‑135 (MQ=255)
                                       gAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGCAGTTcgccgcctt                                                                                                             <  8:41329/149‑4 (MQ=255)
                                        aGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGgtg                                                                                                            <  1:244650/149‑1 (MQ=255)
                                           ccATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTg                                                                                                         >  4:95365/1‑149 (MQ=255)
                                           ccATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTACAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTg                                                                                                         >  7:332330/1‑149 (MQ=255)
                                               gTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCt                                                                                                                                               <  3:175118/107‑1 (MQ=255)
                                               gTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCt                                                                                                                                               >  4:175118/1‑107 (MQ=255)
                                                tCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCa                                                                                                     <  3:52578/148‑1 (MQ=255)
                                                  gACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCCAACACCGCGGCGCAGTTCGccgcct                                                                                                  <  8:315708/149‑5 (MQ=255)
                                                   aCCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTc                                                                                                 >  7:67798/1‑149 (MQ=255)
                                                     cAGGCCGCCAGCGTACTGGTCGCTGGCGACTATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCg                                                                                                >  1:33577/1‑148 (MQ=255)
                                                        gccgccAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAg                                                                                             >  2:239296/1‑148 (MQ=255)
                                                          cgccAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAg                                                                                          >  2:7845/1‑149 (MQ=255)
                                                           gccAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGc                                                                                         >  5:338674/1‑149 (MQ=255)
                                                             cAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGccc                                                                                       <  8:221496/149‑1 (MQ=255)
                                                              aGCGTACTGGTCGCTGGCGATGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGccc                                                                                       >  5:122920/1‑148 (MQ=255)
                                                                   accgcccGCTCGCGACGATCTCCTACAACACCCCCCCCCCCACCACGCGCGCTCCATCCCGATCCCCCAGGGCCGCCAGGCCGTCCTGTTCCTCCTCGAACGCGGCGAACTGCGCCGCGGTGCCGGCCAGCTCGCAGAAGCCCCAgcgc                                                                                 <  8:332330/143‑1 (MQ=255)
                                                                       gTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTtc                                                                                                                                           <  4:137098/87‑1 (MQ=255)
                                                                       gTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTtc                                                                                                                                           >  3:137098/1‑87 (MQ=255)
                                                                       gTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGAt                                                                             <  1:7845/149‑1 (MQ=255)
                                                                              gCGATGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAgg                                                                       <  6:122920/148‑1 (MQ=255)
                                                                              gCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTCGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAgg                                                                       <  1:280117/148‑1 (MQ=255)
                                                                                   gaTTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGc                                                                 >  1:311891/1‑149 (MQ=255)
                                                                                     tttCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCg                                                               <  1:239296/149‑1 (MQ=255)
                                                                                        cGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCATCGCAGATCGCAGGCAATGCCGTa                                                             <  3:95365/148‑1 (MQ=255)
                                                                                         gTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAggcg                                                                                                                              >  5:84042/1‑82 (MQ=255)
                                                                                         gTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAggcg                                                                                                                              <  6:84042/82‑1 (MQ=255)
                                                                                          ttCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCg                                                          >  4:169056/1‑149 (MQ=255)
                                                                                          ttCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCg                                                          >  2:258285/1‑149 (MQ=255)
                                                                                             aaCTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGc                                                       >  2:230463/1‑149 (MQ=255)
                                                                                                  cccGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATgc                                                  >  1:81559/1‑149 (MQ=255)
                                                                                                       cgcTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCg                                             <  8:190216/149‑1 (MQ=255)
                                                                                                       cgcTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCg                                             <  2:81559/149‑1 (MQ=255)
                                                                                                          tGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGc                                           >  8:22955/1‑148 (MQ=255)
                                                                                                            aCCAGGCGTGTTTCATGTCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATCCCGTAGCGGGGGAGGCGCTCGNccac                                        >  8:69990/1‑149 (MQ=255)
                                                                                                            aCCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGccac                                        >  2:163126/1‑149 (MQ=255)
                                                                                                            aCCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGccac                                        <  2:33577/149‑1 (MQ=255)
                                                                                                              cAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATg                                                   >  8:65632/1‑136 (MQ=255)
                                                                                                              cAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGccacca                                      <  6:338674/149‑1 (MQ=255)
                                                                                                              cAGGCGTGTTTCATGCCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATg                                                   <  7:65632/136‑1 (MQ=255)
                                                                                                                 gCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGtt                                   >  5:48685/1‑149 (MQ=255)
                                                                                                                      tttCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGAGGTGTTGGCCAGTTCCCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACt                              >  7:152626/1‑149 (MQ=255)
                                                                                                                             gCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCg                        <  1:163126/148‑1 (MQ=255)
                                                                                                                             gCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCg                        <  7:165485/148‑1 (MQ=255)
                                                                                                                              cGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGAt                      <  1:150772/149‑1 (MQ=255)
                                                                                                                                  ggaacAGGACGACCTGGCCGCCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGt                                    <  8:129897/127‑1 (MQ=255)
                                                                                                                                  ggaacAGGACGACCTGGCCGCCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGt                                    >  7:129897/5‑131 (MQ=255)
                                                                                                                                    cccAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGc                >  8:99351/1‑149 (MQ=255)
                                                                                                                                            ggcCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCa        <  6:48685/149‑1 (MQ=255)
                                                                                                                                            ggcCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCa        <  2:209974/149‑1 (MQ=255)
                                                                                                                                             ggcAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGGCGCGGTGTTGGCGAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCAg       <  3:263545/147‑1 (MQ=255)
                                                                                                                                                aGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCAg       <  6:50373/146‑1 (MQ=255)
                                                                                                                                                aGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCAg       >  5:50373/1‑146 (MQ=255)
                                                                                                                                                  gtcgtcCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCAGGTAgc  >  2:53591/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTTCGCCCTGCACCAGATCCAGCGGGTGCAGGTGGCCCGAGCCCATGTCGACCAGGCCGCCAGCGTACTGGTCGCTGGCGACGATTTCGTTCAACTGCCCGGCGCTGACCAGGCGTGTTTCATGGCGATAGCCCAGGGCGGCCAGGTCGTCCTGTTCCTCCTTGAAGGCGGCGAACTGCGCCGCGGTGTTGGCCAGTTCGCAGAAGCCCCAGCGCAGATCGCAGGCAATGCCGTAGCGGGCGATGCGCTCGGCCACCAGTTCCACTGAGTCGATGCCGGCCTGCTTCAGGTAGC  >  NC_002947/3563240‑3563534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑