Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,588,020	G→A	8.7%	A286T (GCC→ACC)	catA‑II →	catechol 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,588,020	0	G	A	8.7%	127.6 / 2.3	46	A286T (GCC→ACC)	catA‑II	catechol 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (23/19);  new base A (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

GCAGATAAACCTGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCGGGCATGCCCGCGAAGCA  >  NC_002947/3587878‑3588167                                                                                                                                                |                                                                                                                                                     gCAGATAAACCTGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAgg                                                                                                                                                >  4:226093/1‑149 (MQ=255)  cAGATAAACCTGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAggg                                                                                                                                               >  3:140927/1‑149 (MQ=255)        aaCCTGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGc                                                                                                                                         <  6:203361/149‑1 (MQ=255)            tGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGACCCAGGGCGGTGCCGAt                                                                                                                                     >  7:100017/1‑149 (MQ=255)            tGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGACCCAGGGCGGTGCCGAt                                                                                                                                     >  7:105079/1‑149 (MQ=255)              tCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGAt                                                                                                                                     <  8:19831/147‑1 (MQ=255)                      cAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAgcagcg                                                                                                                             >  6:153357/1‑147 (MQ=255)                            ccTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGACCCAGGGCGGTGCCGATGAGCAGCGCAgcgggc                                                                                                                     <  8:100017/149‑1 (MQ=255)                            ccTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGACCCAGGGCGGTGCCGATGAGCAGCGCAgcgggc                                                                                                                     <  8:105079/149‑1 (MQ=255)                                    attaCTTTCCATATGCAACACCGGATGGTATCCACGGGGAGCTCGTGATCGTCGAAGGCCCGGATGGTCGCCATGCCGAGCTGAAGTTCG‑ACTTCCACTTCCAGAAGGCACAGGACCGTGCCCATGAGAAGCGCACCGGCCTGCcgcg                                                                                                              >  4:310864/4‑148 (MQ=255)                                       aCTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCt                                                                                                          <  4:140927/149‑1 (MQ=255)                                               tATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCag                                                                                                    >  3:74844/1‑147 (MQ=255)                                                     acacGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTg                                                                                                       >  5:276255/1‑138 (MQ=255)                                                     acacGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTg                                                                                                       <  6:276255/138‑1 (MQ=255)                                                       acGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGc                                                                                               >  3:182309/1‑144 (MQ=255)                                                       aagggATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGc                                                                                               <  4:182309/142‑1 (MQ=255)                                                           gATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCAc                                                                                      >  2:282652/1‑149 (MQ=255)                                                           gATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCAc                                                                                      <  1:282652/149‑1 (MQ=255)                                                                 cTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCGAAATTACAGTTGCAGCAGGCACAGGGCGGTGCAGATGACCAGCTCAGCGGGCGGCCGCGAGCTTTTCAGCAGGCATGATCCACCGAGt                                                                                 >  1:106842/1‑149 (MQ=255)                                                                      cGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTTCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCttt                                                                                                        <  4:100680/120‑1 (MQ=255)                                                                      cGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCttt                                                                                                        >  3:100680/1‑120 (MQ=255)                                                                        gggAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGtt                                                                         >  1:226256/1‑149 (MQ=255)                                                                              tggtgTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑gg                                                                  <  3:226093/149‑1 (MQ=255)                                                                                gtgtTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑ggg                                                                 >  2:14600/1‑148 (MQ=255)                                                                                      ggcgAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTCCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGccc                                                          <  8:149483/147‑1 (MQ=255)                                                                                          aaGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGACGATGAGCAGCGCAGCGGGCGGCCGCGAGATTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGCGGGGCATGccccac                                                       >  8:223556/1‑146 (MQ=255)                                                                                               ccGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCca                                                   >  4:266956/1‑147 (MQ=255)                                                                                                cGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAg                                                <  4:74844/149‑1 (MQ=255)                                                                                                         ggCATGCCTAGCTGAAGTTGG‑ACTTCCAGTTGCAGCAGGCCCAGGTCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCt                                       <  2:106842/149‑1 (MQ=255)                                                                                                          gCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCtg                                      <  1:14600/149‑1 (MQ=255)                                                                                                          gCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCtg                                      >  2:11596/1‑149 (MQ=255)                                                                                                           cATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCtgt                                     >  3:110271/1‑149 (MQ=255)                                                                                                               ccGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAACAGCGCATAGGGAGGACGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCACACAGGTGAAGACTGTGCAg                                 >  1:274218/1‑149 (MQ=255)                                                                                                                   gCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTcgc                                                                     >  5:173284/1‑110 (MQ=255)                                                                                                                   gCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTcgc                                                                     <  6:173284/110‑1 (MQ=255)                                                                                                                          ttCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCgtt                            >  7:153838/1‑143 (MQ=255)                                                                                                                          ttCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCgtt                            <  8:153838/143‑1 (MQ=255)                                                                                                                               aCTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGa                                            >  3:321246/1‑123 (MQ=255)                                                                                                                               aCTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGa                                            <  4:321246/123‑1 (MQ=255)                                                                                                                               aCTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCgg                  <  4:172178/149‑1 (MQ=255)                                                                                                                               aCTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCgg                  <  3:310864/149‑1 (MQ=255)                                                                                                                               aCTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCgg                  <  3:266956/149‑1 (MQ=255)                                                                                                                                         gcagcaGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCGGGCATGCCcgc        >  5:86916/1‑149 (MQ=255)                                                                                                                                         gcagcaGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCGGGCATGCCcgc        >  7:165513/1‑149 (MQ=255)                                                                                                                                              agGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCtgtg                                    <  2:220469/116‑1 (MQ=255)                                                                                                                                              agGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCtgtg                                    >  1:220469/1‑116 (MQ=255)                                                                                                                                               ggCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCACCCACAGGGGAAGCCAGTGCAGTCGTTGTTTGAGCGGGCATGCCCGCGAagca  >  4:172541/1‑149 (MQ=255)                                                                                                                                               ggCCCAGGGCGGTGCAGATGAGCATCGCAGTGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAATCCTGTGCAGTCGTTGTTGGAGCGGGCATGCCCGCGAagca  <  2:274218/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GCAGATAAACCTGTCGGGGGACAAGTACCTGTGGGATGACTTTGCCTATGCCACACGGGATGGGCTGGTCGGGGAGGTGGTGTTCGTCGAAGGGCCGGATGGTCGGCATGCCGAGCTGAAGTTCG‑ACTTCCAGTTGCAGCAGGCCCAGGGCGGTGCCGATGAGCAGCGCAGCGGGCGGCCGCGAGCTTTGCAGGAGGCCTGATCCACCGAGTGGGCTGTTTCGC‑GGGCATGCCCGCTCCCACAGGTGAAGCCTGTGCAGTCGTTGTTGGAGCGGGCATGCCCGCGAAGCA  >  NC_002947/3587878‑3588167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑