Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,932,640	+C	intergenic (‑107/+43)	rluA ← / ← minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,932,638	1	.	C	100.0%	59.8 / NA	19	intergenic (‑105/+45)	rluA/minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/10);  total (9/10)

CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTG  >  NC_002947/1932500‑1932764                                                                                                                                            |                                                                                                                               cTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAg                                                                                                                        <  3:43384/148‑1 (MQ=255)        gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCTACAAAGGCTATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTg                                                                                                                   >  1:49072/1‑146 (MQ=255)             tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCCAGGCa                                                                                                            >  7:51878/1‑148 (MQ=255)                   cATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGCGCTTGCCCGCGAAGGGCTTCAAGGCAGcccc                                                                                                       >  5:85280/1‑147 (MQ=38)                          cAGCGGCATTGCAGGTCTCACCAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGGCCCCGGTCGGCGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc                                                                                               <  6:174412/148‑1 (MQ=255)                                           tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGCGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCg                                                                              >  7:40939/1‑148 (MQ=255)                                           tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGATCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCATAAGCGGAGCAGCg                                                                              >  5:126388/1‑148 (MQ=37)                                             aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt                                                                            >  6:66681/1‑148 (MQ=255)                                              ctaaatTAGCATTAAACGGGGGGGCGGCTTTGGGGCCCTTCGCGGGCAAGCCCGCTCATAAAATGTATTTGCGTCCCTTGTTGGTGGGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGa                                                                             <  2:49072/144‑1 (MQ=21)                                                         gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTTGGAGCGGGCTTGCCCGCGAAGGGCGGCCAGGCAGCCCCAAATTGTCGACGACGCGGATCAGCGATCCGGCAGGGTGa                                                                >  7:13038/1‑148 (MQ=38)                                                                cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGGTCAGCGCTCCGGCAGGGTGATGTTCAg                                                         >  4:159886/1‑148 (MQ=255)                                                                     gCCGCTTTGCGGCCCGTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAACCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa                                                    <  6:166753/148‑1 (MQ=255)                                                                          tttGCGGCCCGTCGCGGGCAAGCCCGCTCCTACACAGTATAGGCGGCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATGAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                               <  5:138419/148‑1 (MQ=38)                                                                             gCGGCCTTTGGCGGGCAAGCCCGCGCCAACAACGTATTTGCGTCCCTTGTAGTAGGGGGCTGGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa                                             <  8:29483/147‑1 (MQ=255)                                                                                              aGCCCGCTCCTAAAATGTATATGCGTCCCGTTTTGTCGGGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                           <  5:100967/148‑1 (MQ=255)                                                                                                cccGCTCCTACAACGCATATGCGCCCCCTGAAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa                         <  5:153889/148‑1 (MQ=39)                                                                                                   gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                           >  8:599/1‑143 (MQ=255)                                                                                                    cTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                          <  7:599/143‑1 (MQ=255)                                                                                                                       tCCCGTGTTGGAGCGGGGTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGGGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg  <  6:85280/148‑1 (MQ=39)                                                                                                                                            |                                                                                                                               CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTG  >  NC_002947/1932500‑1932764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑