Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,845,705	+G	intergenic (‑72/+26)	PP_3394 ← / ← PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,845,703	1	.	G	100.0%	43.9 / NA	14	intergenic (‑70/+28)	PP_3394/PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/3);  total (11/3)

TGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCA  >  NC_002947/3845562‑3845836                                                                                                                                               |                                                                                                                                      tgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggctg                                                                                                                                 >  5:50819/1‑144 (MQ=255) tgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc                                                                                                                                   <  6:44431/146‑3 (MQ=255)   tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGAt                                                                                                                                >  8:82403/1‑148 (MQ=255)      gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGTGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCa                                                                                                                             >  3:143313/1‑148 (MQ=255)               cTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGTGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGc                                                                                                                     <  4:143313/147‑1 (MQ=255)                ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCa                                                                                                                   >  8:102851/1‑148 (MQ=255)                      gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATgg                                                                                                             >  3:26643/1‑148 (MQ=255)                             aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGcc                                                                                                      >  6:32758/1‑148 (MQ=255)                                             cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGcc                                                                                      >  5:31172/1‑148 (MQ=255)                                                               ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGCTGGCTCAGCCCGCGCCCTGCCAGGCCGGTGGACGCAGCAACAgg                                                                    >  3:260/1‑148 (MQ=255)                                                                                     gtctgACGCGTTGCGCCAGACTAAGCGCGGGATGACGGGCAGAAAACACGTGTTGCGCGGGCTGATCCATCGCCAGCCACGAGGGATCCGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                                <  7:129443/146‑1 (MQ=255)                                                                                                tGCGCCCGACTAAGCGCGGGATGACGGGCAGAAACGCCGGGTTTCGCGGGCTGAGCCATCGCCCGCCCCGAGGCATCCGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGc                                   <  7:82403/148‑1 (MQ=255)                                                                                                                  ggATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCCCGCAGCAACAGGTGGTCAAGCCGGCGCCGAGCCGCCAGCGAGAGCGTGTGCCGGTCGCGCAt                  >  5:134150/1‑147 (MQ=255)                                                                                                                      gACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGa             >  4:169472/1‑148 (MQ=255)                                                                                                                               aaaaGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt     >  8:36352/1‑147 (MQ=255)                                                                                                                                 aaGACGTGTTTCGCGGGCTGATCCATCGTCAGCCCCGATGGCTCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGCGCCGCCAGCGCGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCa  >  3:7838/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                      TGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCA  >  NC_002947/3845562‑3845836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑