| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,809 | 0 | G | A | 30.4% | 40.4 / 12.1 | 24 | intergenic (+103/‑443) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (5/11); new base A (4/3); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGG > NC_002947/5988668‑5988954 | gTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTc < 5:87899/148‑1 (MQ=255) ctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTgg < 2:162879/148‑1 (MQ=255) agcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGa < 6:155129/146‑1 (MQ=255) ggATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCt > 2:22233/1‑148 (MQ=255) tgcattttGGCGCGGGCATTGAGGTTGGTAGTGCCGGTGAGATTGGGCGCCGCCCGCGAGGCGATGCGCGGGTAAACCCGCTCCTAAGTGTGTTTCGGGCCATTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCActcctc < 6:37238/143‑1 (MQ=255) cgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcgt < 3:14476/148‑1 (MQ=255) gTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTACACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg < 5:82382/148‑1 (MQ=255) ttgccgcgCCTGGGAGAGCGCGCCCCGCCCGCGCGGCGCTTCGCGGGTACACCCGCTCCTACGGTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCgg < 4:35583/141‑1 (MQ=12) tAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTc < 5:170507/147‑1 (MQ=255) cgCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCAACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc < 2:16328/147‑1 (MQ=11) tGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGcaca < 1:22233/148‑1 (MQ=255) cgccgcCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCTGGCCAATCCCTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggag < 3:130775/148‑1 (MQ=25) gccgccCGCGCGGCGCGTCGCGGGTAACCCCGCTCCTACGTTTGGTTCGGGCCAGTTATTCCTGTGGGCCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagg < 8:152818/148‑1 (MQ=255) gcgcgGCGCTTCGCGGGTAAACCCGCGCCTACGTTTGTTTCGGGCCAGTTAATCCTGTGGGACGCGCACGCGAGCCCCCGGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc < 2:107229/148‑1 (MQ=255) ttCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaa > 4:129266/1‑148 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTTTTCCTGCGGGATTTGCGCGCGCCCGCCTTGGGGCATGTCTCGATCTCGCGTCGTACAAACAAGGCGGTCGCGGGCGACTGGTGCAGGAGGGACTGG‑CGGAAACAAACg > 5:45130/1‑148 (MQ=2) gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 8:67364/147‑1 (MQ=255) ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg > 4:88907/1‑148 (MQ=255) cGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt > 8:52929/1‑148 (MQ=255) tACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCCCGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAccc > 3:129266‑M2/1‑145 (MQ=255) tttgtttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GCAACATACGTAGGAGCGGGTTTACCCGCGac > 4:20454/1‑147 (MQ=2) cGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccgc > 8:100359/1‑148 (MQ=14) gCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCcgcgcg > 2:168499/1‑148 (MQ=14) cAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgg < 2:54228‑M2/148‑20 (MQ=255) | GTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGG > NC_002947/5988668‑5988954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |