Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,842,194	C→A	G867V (GGT→GTT)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,842,194	0	C	A	100.0%	105.3 / NA	32	G867V (GGT→GTT)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/18);  total (14/18)

ATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAG  >  NC_002947/1842051‑1842336                                                                                                                                                |                                                                                                                                               aTCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACg                                                                                                                                              <  7:57193/146‑1 (MQ=255) aTCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGt                                                                                                                                             >  8:151320/1‑147 (MQ=255)              cgccAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTAc                                                                                                                                <  3:70751/147‑1 (MQ=255)               gccAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCTGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCt                                                                                                                              >  6:10677/1‑148 (MQ=255)               gccAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCt                                                                                                                              >  4:43070/1‑148 (MQ=255)                   gCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTc                                                                                                                          <  7:152260/148‑1 (MQ=255)                   gCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTc                                                                                                                          <  8:158258/148‑1 (MQ=255)                         tGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGCCGGTGTACCTGCTCGATCAg                                                                                                                    >  1:150760/1‑148 (MQ=255)                         tGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAg                                                                                                                    <  7:92450/148‑1 (MQ=255)                         tGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTCAGGCAACGTTCAGACGGTGTACCTGCTCGATCAg                                                                                                                    >  6:21165/1‑148 (MQ=255)                            tCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGc                                                                                                                  <  4:88209/147‑1 (MQ=255)                                  tGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCg                                                                                                           <  5:58640/148‑1 (MQ=255)                                      cAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACTCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTc                                                                                                        >  8:153798/1‑147 (MQ=255)                                                aGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTgcgc                                                                                             <  2:35621/148‑1 (MQ=255)                                                 gggCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGcgcg                                                                                            <  6:55778/148‑1 (MQ=255)                                                         tgcGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGgcgc                                                                                    <  8:142160/148‑1 (MQ=255)                                                             ccTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTAt                                                                                <  6:13277/148‑1 (MQ=255)                                                              cTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTg                                                                                                                             >  6:68122/1‑102 (MQ=255)                                                               tGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGc                                                                                                                            <  5:68122/102‑1 (MQ=255)                                                                   gATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCTCGGGCGGCGCGTATGGCTTc                                                                          <  8:10939/148‑1 (MQ=255)                                                                   gATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTc                                                                          <  3:69871/148‑1 (MQ=255)                                                                          cTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTcc                                                                   >  8:5526/1‑148 (MQ=255)                                                                          cTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTcc                                                                   >  3:24172/1‑148 (MQ=255)                                                                           tGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTcc                                                                   >  2:87701/1‑147 (MQ=255)                                                                                      ggTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAg                                                       <  5:111907/148‑1 (MQ=255)                                                                                            gCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCca                                                   >  7:21463/1‑146 (MQ=255)                                                                                              tGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCcagca                                                >  4:11156/1‑147 (MQ=255)                                                                                                              ccGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTcgcg                                                                       >  3:74580/1‑108 (MQ=255)                                                                                                               cGTAACTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCgg                                                                      <  4:74580/108‑1 (MQ=255)                                                                                                                            cgccgcAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGc                 <  5:126062/148‑1 (MQ=255)                                                                                                                              ccgcAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCt               >  1:159431/1‑148 (MQ=255)                                                                                                                                            ggCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAg  <  7:151320/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               ATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAG  >  NC_002947/1842051‑1842336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑