Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,629,711	G→T	G223V (GGC→GTC)	clpX →	ATP‑dependent Clp protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,629,711	0	G	T	100.0%	85.0 / NA	26	G223V (GGC→GTC)	clpX	ATP‑dependent Clp protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/10);  total (16/10)

AGAACATCATCCAGAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGGCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACATCCTGTTCATCTGTGGTGGTGCGTTCTCGGGCC  >  NC_002947/2629570‑2629854                                                                                                                                              |                                                                                                                                                agaACATCATCCAGAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTgca                                                                                                                                           <  5:171333/148‑1 (MQ=255)   aaCATCATCCAGAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTgcagc                                                                                                                                         <  7:70681/148‑1 (MQ=255)      atcatcCAGAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGcagcag                                                                                                                                       >  8:106325/1‑147 (MQ=255)          tcCAGAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCAct                                                                                                                                  >  2:165674/1‑148 (MQ=255)              gAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCActgct                                                                                                                               >  1:51893/1‑147 (MQ=255)              gAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCActgct                                                                                                                               >  3:11620/1‑147 (MQ=255)                         aGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGa                                                                                                                   <  7:106325/148‑1 (MQ=255)                                          gTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCgg                                                                                                  >  6:103871/1‑148 (MQ=255)                                            ggAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGtt                                                                                                >  3:96179/1‑148 (MQ=255)                                               aaaGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTccg                                                                                             >  1:179234/1‑148 (MQ=255)                                                     ccAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAg                                                                                       >  5:83710/1‑148 (MQ=255)                                                     ccAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAg                                                                                       >  5:155473/1‑148 (MQ=255)                                                                ttGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCaa                                                                            <  5:103871/148‑1 (MQ=255)                                                                  gTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAgg                                                                                      >  6:132698/1‑136 (MQ=255)                                                                   tCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAggg                                                                                     <  5:132698/136‑1 (MQ=255)                                                                        aTCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCg                                                                     >  3:169891/1‑147 (MQ=255)                                                                                aaTCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGaa                                                            <  5:112238/148‑1 (MQ=255)                                                                                aaTCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGaa                                                            <  4:11620/148‑1 (MQ=255)                                                                                     aCAAGATTTCGCGCAAGTCGGACAACCTGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCt                                                       >  1:60694/1‑148 (MQ=255)                                                                                         gATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGTCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAg                                                   <  6:83710/148‑1 (MQ=255)                                                                                               gcgcAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGa                                              <  5:29688/147‑1 (MQ=255)                                                                                                         gACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACa                                   >  8:28508/1‑148 (MQ=255)                                                                                                                          ccGTGATGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACATCCTGTTCATCTgtgg                   <  4:169891/147‑1 (MQ=255)                                                                                                                                tGTCTCGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACATCCTGTTCATCTGTGGTGGTGCg            >  4:73986/1‑148 (MQ=255)                                                                                                                                    tcGGGCGAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACATCCTGTTCATCTGTGGTGGTGCGTtct        >  4:10870/1‑148 (MQ=255)                                                                                                                                          gAGGTCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACATCCTGTTCATCTGTGGTGGTGCGTTCTCGGGcc  >  6:37904/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                                AGAACATCATCCAGAAGCTGTTGCAGAAGTGCGACTACGACGTGGAAAAGGCCCAGATGGGCATTGTCTACATCGACGAAATCGACAAGATTTCGCGCAAGTCGGACAACCCGTCCATTACCCGTGATGTCTCGGGCGAGGGCGTGCAGCAGGCACTGCTGAAACTGATCGAGGGCACCGTGGCCTCGGTTCCGCCGCAGGGCGGCCGCAAGCACCCGCAACAGGAATTCCTGCAGGTTGATACCCGCAACATCCTGTTCATCTGTGGTGGTGCGTTCTCGGGCC  >  NC_002947/2629570‑2629854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑