Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,741,331	G→A	A201V (GCG→GTG)	PP_3305 ←	TerC family membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,741,331	0	G	A	100.0%	54.1 / NA	17	A201V (GCG→GTG)	PP_3305	TerC family membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/11);  total (6/11)

GATGAACGGATCCTGTGTGATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACGCATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCAAGTCAGGCGCATGATCGACGCGAGCGAATAGCATCTTGACC  >  NC_002947/3741204‑3741465                                                                                                                                |                                                                                                                                       gATGAACGGATCCTGTGTGATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATcac                                                                                                                     >  5:138201/1‑147 (MQ=255)   tGAACGGATCCTGTGTGATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACaaa                                                                                                                  <  3:11492/148‑1 (MQ=255)    gAACGGATCCTGTGTGATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAg                                                                                                                 >  7:134432/1‑148 (MQ=255)        ggATCCTGTGTGATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGtt                                                                                                             <  2:123293/148‑1 (MQ=255)               gtgtgATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGt                                                                                                              >  5:31721/1‑140 (MQ=255)                tgtgATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGtt                                                                                                             <  6:31721/140‑1 (MQ=255)                                    gCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGCATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAg                                                                                 >  3:108386/1‑148 (MQ=255)                                                cAGCAAATACCATGTCTGCACACTCAATCAAAATAAGTGCAAGTAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGTCAAGCGAATGACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTTTGTGCGCat                                                                     <  6:127729/148‑2 (MQ=255)                                                 aGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAgg                                                                    <  7:41578/148‑1 (MQ=255)                                                                           tcaaAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCaa                                          <  5:62032/148‑1 (MQ=255)                                                                           tcaaAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCaa                                          <  3:150670/148‑1 (MQ=255)                                                                                          ggAAGAGCGGTGTCACCCATAGCATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCAAGTCAGGCGcatcata                           >  3:3594/1‑147 (MQ=255)                                                                                                   gtgtCACCCATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCTAAGTCAGGCGCATGATCGACgcgagc                  <  6:138201/148‑1 (MQ=255)                                                                                                         cgcATAGAATTCTTTTTCCTGAGACATCGGGCAAGCGATTCACAATGCGTTGAGCGTGCAATTGTTTGGTGATGCGTTGGTGTGTGCGCAGGTTGTTGACGATACGGTTGTTTTCCAAGTCAGGCGCATGATCGAGGCGAGCGtata             <  4:35858/145‑1 (MQ=255)                                                                                                         cccATAGAATTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCAAGTCAGGCGCATGATCGACGCGAGCGAATa             <  8:134432/147‑1 (MQ=255)                                                                                                                 aTTCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCAAGTCAGGCGCATGATCGACGCGAGCGAATAGCATCTTGa    >  6:33567/1‑148 (MQ=255)                                                                                                                   tCTTTTTCCTGACACATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCAAGTCAGGCGCATGATCGACGCGAGCGAATTGCATCTTGAcc  <  3:124401/148‑1 (MQ=255)                                                                                                                                |                                                                                                                                       GATGAACGGATCCTGTGTGATAGCAAAAATCGCAGGCACGCTGTCAACAGCAAATACCAAGTCTGCACACTCAATCAAAATAAGTGCAAGGAAGAGCGGTGTCACCCATAGAATTCTTTTTCCTGACGCATCGGGCAAGCGAATCACAAAGCGTTGAGCGTGCAATTCTTTGGTGATGCGTAGGTGTGTGCGCAGGTAGTTGACGAAACGGTTGTTTTCCAAGTCAGGCGCATGATCGACGCGAGCGAATAGCATCTTGACC  >  NC_002947/3741204‑3741465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑