Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	94.4 / NA	28	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499059‑499346                                                                                                                                                 |                                                                                                                                                aGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGc                                                                                                                                              <  7:33593/148‑1 (MQ=255)    ccATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                                           <  5:169294/148‑1 (MQ=255)          gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAATCCcgcc                                                                                                                                     >  1:70999/1‑147 (MQ=255)            aCCCACACTGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGGCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGaa                                                                                                                                   <  2:123120/148‑1 (MQ=255)              ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCCAGCCcgcgcccg                                                                                                                                 >  1:99591/1‑144 (MQ=255)              ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACg                                                                                                                                 >  7:57149/1‑148 (MQ=255)                acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACggg                                                                                                                               >  2:18707/1‑148 (MQ=255)                acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACggg                                                                                                                               >  5:86067/1‑148 (MQ=255)                   aGGCCCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                            <  3:117308/148‑1 (MQ=255)                       actccTGATGCAGAAGGGGAAAGTGAACGCGCTGGCTGAGCTGGTGAAGTACGCCATGGCGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCTAACGggcctggcc                                                                                                                        <  8:14952/145‑1 (MQ=255)                            tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCcggctg                                                                                                                   >  7:155157/1‑148 (MQ=255)                             gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                                      >  7:54204/1‑128 (MQ=255)                              aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                                     <  8:54204/128‑1 (MQ=255)                                    aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAt                                                                                                           <  2:52227/148‑1 (MQ=255)                                           aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTTATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGa                                                                                                     <  2:36828/147‑1 (MQ=255)                                              tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg                                                                                                 <  1:87013/148‑1 (MQ=255)                                                 aCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGcc                                                                                              <  2:164517/148‑1 (MQ=255)                                                          cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACTGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                     <  8:159450/148‑1 (MQ=255)                                                                                 gAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGCGCCGGCCTCTTAGCGGGCATTCCCGCTCCCACAGGTTTc                                                              >  6:68677/1‑148 (MQ=255)                                                                                         gCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCCCACCGGCCTGGCCGGGGGGTCTCGTTGGGGGCGCCGGGCCCTTTGCGGGCCTGCCCGCGCCCACACGGTTGgtgggtgg                                                      >  5:33045/1‑148 (MQ=255)                                                                                                    tGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGCCGGGCCGGCGGAACTCTATGGCTGCGCCGGCCTCTTAGCCGGCATTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGc                                           >  6:84302/1‑148 (MQ=255)                                                                                                                    ccGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCCCCAACCGGCCGGGGCTGCGGAACAGTATTGCTGAGCCCGCCAAATAGCCGGCAAGCCCCCAACCCCCCTTTTAGTGGTTGGGTCCCCAAGGCAGTGCAATCCCGccca                           >  4:85359/1‑146 (MQ=255)                                                                                                                         ccTTGCCGAAGCCTATGTGGGAGCGGGCAGGCCCGCTACGGGGCCTGTCCGGCTTAGCTCTATGGCGGAGCCGGCCTGTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTg                      <  8:57149/147‑1 (MQ=255)                                                                                                                         aCTTGCCGACGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGGTGATCTCTATGGCTGAGCGGGCCTCTTCGGGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTg                      <  8:155157/148‑1 (MQ=255)                                                                                                                           ttGCCGAAGCCTTTGTGGGAGGGGGCAGGCCGGCGAACGGGCGTGGCGTGCTTATCTATATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa                    <  8:149013/148‑1 (MQ=38)                                                                                                                                      tATGTGGGAGCGTGCATGCCCGCGAACGGGCCTGGCCTGCTGATATCTATGGCTGAGCCGGCCTCTTAGCGGGCATGCCCGCTCCCACAGGTTTAGTGGTTGGCTAGCAAGGGCATTCCAATCCCGCCATAATTGTAGGGCGATccc          >  6:39879/1‑147 (MQ=38)                                                                                                                                       aTGTGGGAGCGGGCATTCCCGCGAACCGGCCTGGCCTGCTGATCTCTATTGCGGAGCCGGCCTCTTAGCGGGCATGCCCGCTACCACACGTTTAGTGGTTTGCTCGCAAGGGCCATGCAATCCCGCCATACATTTAGGGCGATACCt         >  8:39062/1‑147 (MQ=255)                                                                                                                                             gAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAGGGCTGAGCCGGCCTCTGCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGATCGCCCTacaagga  <  5:84302/148‑4 (MQ=38)                                                                                                                                                 |                                                                                                                                                AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499059‑499346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑