Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 541,625 C→T P1335S (CCC→TCC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947541,6250CT97.3% 119.5 / NA 37P1335S (CCC→TCC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/16);  minor base G (0/1);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGCCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCAGGAGGAAAGGCCTTGAAAG  >  NC_002947/541482‑541767
                                                                                                                                               |                                                                                                                                              
agaTGTAAGTGTGGGCGCTGGAAGCATACGGCTCGGCATACACCCTGCAAGAATTGCTCACAGTGAAGTCTGACGTCTTGACCGGCCGTACCACGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGTGCCGGGCATGGCCGa                                                                                                                                            <  5:118741/148‑1 (MQ=255)
agaTGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGa                                                                                                                                            <  5:93077/148‑1 (MQ=255)
         tgtgGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCaa                                                                                                                                   <  1:90316/148‑1 (MQ=255)
              gcgcTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGTGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACgtgt                                                                                                                              <  1:166380/148‑1 (MQ=255)
               cgcTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGtt                                                                                                                             <  5:58547/148‑1 (MQ=255)
                 cTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGa                                                                                                                           <  2:2653/148‑1 (MQ=255)
                  tGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGa                                                                                                                           >  5:69585/1‑147 (MQ=255)
                     aaGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCACCGTGTTGATc                                                                                                                         >  2:39885/1‑146 (MQ=255)
                         aTACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAagag                                                                                                                   <  3:129863/148‑1 (MQ=255)
                                 cgGCATACGCCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTc                                                                                                           >  4:119185/1‑148 (MQ=255)
                                      tACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCg                                                                                                      <  7:8086/148‑1 (MQ=255)
                                      tACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCg                                                                                                      >  4:153218/1‑148 (MQ=255)
                                        cacCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGt                                                                                                    >  5:138699/1‑148 (MQ=255)
                                         acCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATc                                                                                                                >  6:118395/1‑135 (MQ=255)
                                          cccTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATcc                                                                                                               <  5:118395/135‑1 (MQ=255)
                                                       gCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTg                                                                                     <  6:69585/148‑1 (MQ=255)
                                                         tCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTgg                                                                                    >  3:112383/1‑147 (MQ=255)
                                                           acaGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGCTATCGATCTGGaaa                                                                                 >  1:39216/1‑148 (MQ=255)
                                                             aGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAAc                                                                                >  2:37040/1‑147 (MQ=255)
                                                                  aGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAAt                                                                           <  2:94271/147‑1 (MQ=255)
                                                                    tCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAac                                                                        >  2:146950/1‑148 (MQ=255)
                                                                       gacgacGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAAcgcg                                                                     >  5:30216/1‑148 (MQ=255)
                                                                                 aCGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAg                                                           <  3:153218/148‑1 (MQ=255)
                                                                                     ccGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGa                                                       >  1:79004/1‑148 (MQ=255)
                                                                                              gATGTACAAGAACATCGGGGATGGCGATCACAGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGCTCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAAGACGTGACGCGAAGGGGAGTGGGGCAg                                              <  5:164262/148‑1 (MQ=255)
                                                                                                    cAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATg                                        >  1:58033/1‑148 (MQ=255)
                                                                                                     aaGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGc                                       >  7:111559/1‑148 (MQ=255)
                                                                                                      aGAACATCGTGGATGGCGATCACAGTATGGAGCCGGGCATGTCCGAGTCCTTCATCGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATTTCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGcc                                      <  8:60476/148‑1 (MQ=255)
                                                                                                       gAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGccc                                     >  1:11126/1‑148 (MQ=255)
                                                                                                       gAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGccc                                     >  7:26730/1‑148 (MQ=255)
                                                                                                        aaCATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCg                                    >  4:132201/1‑148 (MQ=255)
                                                                                                                   tGGCGATCACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTcc                         <  3:39229/148‑1 (MQ=255)
                                                                                                                          cACCGTATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCagga                  <  6:138699/148‑1 (MQ=255)
                                                                                                                               tATGGAGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCAGGAGGaaa             >  5:156836/1‑148 (MQ=255)
                                                                                                                                    aGCCGGGCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCAGGAGGAAAGGCCt        >  3:60086/1‑148 (MQ=255)
                                                                                                                                        gggCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCAGGAGGAAAGGCCTTg      >  3:60600/1‑146 (MQ=255)
                                                                                                                                          gCATGTCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCAGGAGGAAAGGCCTTGAAAg  <  1:20871/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
AGATGGAAGTGTGGGCGCTGGAAGCATACGGCGCGGCATACACCCTGCAAGAAATGCTCACAGTGAAGTCGGACGACGTGAACGGCCGTACCAAGATGTACAAGAACATCGTGGATGGCGATCACCGTATGGAGCCGGGCATGCCCGAGTCCTTCAACGTGTTGATCAAAGAGATCCGTTCGCTCGGTATCGATATCGATCTGGAAACCGAATAACACGTGACGCGAAGGGGAGTGGGGCAGGTAATGCCCGCTCCCTGCTCCGCCAGGAGGAAAGGCCTTGAAAG  >  NC_002947/541482‑541767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑