| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 92.3% | 103.2 / 4.2 | 39 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (13/23); total (15/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499363‑1499644 | tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCCGTGT‑TAGCCGGCAcg‑c‑ccc > 8:256634/1‑147 (MQ=37) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTGT‑TAGCGGGCAcg‑c‑ccgcgccc > 2:206569/1‑142 (MQ=25) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGa < 3:291243/148‑1 (MQ=21) cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCTCCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGc < 5:311871/149‑1 (MQ=25) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACCCGCGTTGCCGGTGT‑TAGCGGGCACG‑CTC‑GCTCCCACAGACTGAATGCCAtt > 2:44851/1‑148 (MQ=25) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTaa < 1:267108/149‑1 (MQ=21) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACACGCGCCGCGGGGCATGGCACCCGCGTTTCCGGTGT‑TAGCGGGCACGCC‑C‑CCGCCCCCCCACTGATTGCCAATAAGc > 4:117138/1‑149 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGc > 5:144613/1‑149 (MQ=21) cttaaCGAAGCCTTGGCACCACAGCTGGATTGAGGCTGCCAATCGTTGGGAGCGGGCGTGCCCGCTAAACTGGCGCCGCGGGGCATGTCACCGGATTTGCCGGTGT‑TCGCGGTCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCgg < 4:250370/146‑1 (MQ=18) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCgg < 1:206569/149‑1 (MQ=21) aaCGAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGGGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGCCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGc < 5:19766/148‑1 (MQ=25) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCCGGCCTGCCCCCGCAACAGGCCCCGCGGTGCATGGCACCGGCATTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACAGAATGCCATTAAGCggggc > 5:167496/1‑147 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 6:103222/149‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg > 7:221092/1‑149 (MQ=21) cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 5:306495/149‑1 (MQ=21) gcagGCCTGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGAAACACGCGCCCAGCGGCATGGCACCGGCAGAGCCGGTGT‑TAGCGGGCACGCC‑C‑GCTCCCACACGCTGATTGCCAATTAGAGGGGCTGTCGCAACAGGGTCGGgcc > 2:256758/1‑149 (MQ=255) tGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCCCGGTGCATGGCACCCGCGTTGCCGGTGT‑TAGCCGGCCCGCC‑C‑GCGCCCCCCGACTGAATGCCATTAAGCCGGGCGATCGGTACAGTGGCGGCCCGgttttt > 3:283999/1‑149 (MQ=16) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 6:144613/147‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 8:221092/147‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 4:291614/148‑1 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 3:117138/149‑1 (MQ=21) gggcgggcCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGGTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 4:43709/149‑1 (MQ=37) cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACG‑CTC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 1:44851/149‑1 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 7:232972/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCTTGGCACCGGCTTGGCCGGTGTGC‑GCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTAGCCTCGCTAACGAATCATGACTCCCTTa < 1:193295/149‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTAACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 5:280091/149‑1 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGTACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCGTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 7:265414/127‑1 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGTACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc > 8:265414/1‑127 (MQ=25) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 6:2110/1‑148 (MQ=37) ctaaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 1:321244/147‑1 (MQ=37) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt < 5:2110/149‑1 (MQ=37) aTGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 3:17363/98‑1 (MQ=21) aTGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 4:17363/1‑98 (MQ=21) aTGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACG‑C‑CAGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa > 2:214489/1‑148 (MQ=38) gcaccGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCCATc > 7:163753/5‑133 (MQ=37) gcaccGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCCATc < 8:163753/129‑1 (MQ=37) accGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 2:8424/3‑149 (MQ=38) ggCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc > 5:77251/1‑148 (MQ=39) ttcgcGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa < 7:256634/145‑1 (MQ=255) cgcGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg > 4:251195/3‑149 (MQ=255) cgcGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc < 1:8424/146‑1 (MQ=255) | TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499363‑1499644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |