| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 307,866 | +C | intergenic (+881/‑988) | hslO → / → PP_0254 | molecular chaperone HslO/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 307,865 | 1 | . | C | 98.1% | 176.8 / ‑3.4 | 53 | intergenic (+880/‑989) | hslO/PP_0254 | molecular chaperone HslO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (22/30); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT > NC_002947/307719‑308008 | aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTTTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑cc < 8:309230/149‑2 (MQ=255) ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgt > 7:249641/1‑146 (MQ=255) gTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTAcc > 8:204358/1‑149 (MQ=255) tctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑t > 2:219309/1‑148 (MQ=255) ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGAGTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGAt < 1:219309/147‑1 (MQ=255) ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATc < 6:181500/148‑1 (MQ=255) tGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGt < 7:308678/149‑1 (MQ=255) cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTga > 2:43398/1‑149 (MQ=255) ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTg < 1:43398/149‑1 (MQ=255) ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTggg < 6:287974/149‑1 (MQ=255) ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgtt < 1:97159/119‑5 (MQ=255) ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgtt > 2:97159/1‑115 (MQ=255) aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc > 2:186674/1‑149 (MQ=255) gTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt > 6:151001/1‑149 (MQ=255) tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt < 2:15731/149‑1 (MQ=255) acagCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGCCCTAGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTATCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACTAGCTCGGTTGGGGCGAAGGCGTTg > 2:107301/4‑149 (MQ=255) cTGCCTATGCACTGCGCGGCCAACATCAGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt > 8:90847/1‑149 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGt > 6:62843/1‑114 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGt < 5:62843/114‑1 (MQ=255) ttggaCTGCGCGGCCAACATCGGCGAAGAAGGCGAGGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGACA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTggg < 2:111941/126‑1 (MQ=255) aTGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa < 7:139917/149‑1 (MQ=255) aTGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa > 1:302621/1‑149 (MQ=255) aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt < 6:308031/137‑1 (MQ=255) aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt > 5:308031/1‑137 (MQ=255) cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAg > 4:315502/1‑149 (MQ=255) cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct > 6:122400/1‑149 (MQ=255) aaaCATCGGCGAAGAACGCGATGTTACCTTGTTCTTAGTACTTTACGGCACTGTCAAGACTACACTTTCGTCTGACGAAT‑GCCGTTACC‑TTATCGGTGACGACGAGCACGGTTGGGGCTAAGGCGTTGTCTTCAACATGTAAGGCGtct < 4:215881/148‑1 (MQ=255) tCGGCGAATAAGGCGTTGGGACCTTGTTCTTCGGCCTGTGCGTCAATGGCAAGACTACCCTTTCGGCTGACGAAATC‑CGTTACCATGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa < 1:272199/149‑1 (MQ=255) gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc > 7:268927/1‑148 (MQ=255) gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa < 8:249641/149‑1 (MQ=255) aagaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg < 1:134470/149‑1 (MQ=255) gaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTg < 2:302621/149‑1 (MQ=255) gATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa > 5:193859/1‑149 (MQ=255) gATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa > 5:189332/1‑149 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 3:315502/149‑1 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 7:204358/149‑1 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 8:259381/149‑1 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 6:193859/149‑1 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 6:189332/149‑1 (MQ=255) tgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCt < 8:156732/148‑1 (MQ=255) cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACAAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc > 6:108487/1‑148 (MQ=255) ttGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc > 1:305785/1‑147 (MQ=255) cttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag > 7:59276/1‑148 (MQ=255) cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc < 5:151001/148‑1 (MQ=255) cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACAAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc < 5:108487/148‑1 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa > 8:42318/1‑98 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa < 7:42318/98‑1 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACg < 2:316288/148‑1 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGa < 5:228402/149‑1 (MQ=255) gCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAg > 7:29975/1‑149 (MQ=255) cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa > 5:264921/1‑102 (MQ=255) cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa < 6:264921/102‑1 (MQ=255) cTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTgg < 5:122400/149‑1 (MQ=255) cAAGACTACCCTTTCGGCTGACTAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGaaa < 2:305785/148‑1 (MQ=255) aaGACTACCCTTTCGGCTGACGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg < 2:233272/136‑1 (MQ=255) aCGAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTa > 7:17906/1‑149 (MQ=255) gAAA‑GCCGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATGGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt < 8:29975/149‑1 (MQ=255) | AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACC‑TGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT > NC_002947/307719‑308008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |