Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,126,647 +C intergenic (‑101/+48) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,126,6451.C96.4% 91.1 / NA 29intergenic (‑99/+50)tdcG‑II/gcvP‑IL‑serine dehydratase/glycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base C (13/14);  minor base G (0/1);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA  >  NC_002947/1126519‑1126787
                                                                                                                                |                                                                                                                                              
tGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAATGCGGGGGGTTAGCCACCATAGCAATAGCGGG‑GAAACCCCCGCCGAAACAGAGccccc                                                                                                                           >  4:69787/1‑147 (MQ=255)
          gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTAACGCGAAATGGGAGGGTTAGCCACCAAAGCAATAGCGGGTTAAA‑CCCCGCCAACACAGAGCCCCCCAccccaac                                                                                                                  >  7:130/1‑144 (MQ=255)
            aGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAg                                                                                                               <  4:54245/149‑1 (MQ=255)
               cGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCTAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGt                                                                                                             <  4:40637/148‑1 (MQ=255)
                       gACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa                                                                                                     <  6:104618/148‑1 (MQ=255)
                            gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCCCCAACGCCTTAGCGGG‑GAAACCCGCTCCGACCCAGAGCCCCCCCCCCCAGCCCGGTGGCGGGGAGGGCTa                                                                                               >  6:175033/1‑149 (MQ=37)
                                        ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCTAATGCGGAGTTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc                                                                                     <  2:196175/147‑1 (MQ=255)
                                         cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa                                                                                  <  4:100320/149‑1 (MQ=255)
                                                         cATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAc                                                                   <  8:130/148‑1 (MQ=255)
                                                                  cTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgcgc                                                         <  5:175033/149‑1 (MQ=255)
                                                                     tAGGGGGGGGTTTACCCGGTATTGCGTTGTTATAGCCACCAGCGATTTCGGGGT‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcac                                                       <  6:223444/148‑1 (MQ=255)
                                                                         cgcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg                                                  >  7:65750/1‑149 (MQ=255)
                                                                                    ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                       >  5:301157/1‑149 (MQ=255)
                                                                                    ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                       >  6:134372/1‑149 (MQ=255)
                                                                                     cgcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcac                                                       <  7:250236/132‑1 (MQ=255)
                                                                                     cgcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcac                                                       >  8:250236/1‑132 (MQ=255)
                                                                                      gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCg                                     <  8:65750/149‑1 (MQ=255)
                                                                                             cGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg                               <  5:134372/148‑1 (MQ=255)
                                                                                              ggAGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt                             <  3:69787/149‑1 (MQ=255)
                                                                                                aGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGt                           <  5:89179/149‑1 (MQ=255)
                                                                                                aGGTATAGCCACCATCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGt                           >  3:88232/1‑149 (MQ=255)
                                                                                                        ccaccaTCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGTCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg                   >  8:297311/1‑149 (MQ=255)
                                                                                                        ccaccaTCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTATCGACCCgg                   >  3:46801/1‑149 (MQ=255)
                                                                                                           ccaTCGCATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGcc                 >  6:174907/1‑148 (MQ=255)
                                                                                                                 cATTCGCGGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc          >  7:61960/1‑149 (MQ=255)
                                                                                                                     cgcgGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc       >  7:252625/1‑148 (MQ=255)
                                                                                                                      gcgGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc       >  6:194888/1‑147 (MQ=255)
                                                                                                                        ggg‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGGGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGt   <  5:194888/149‑1 (MQ=255)
                                                                                                                         gg‑TAAAGCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGTGAGGGCTATCAGGCGTCCTGATAGCTCTCGAGCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa  <  5:214718/149‑1 (MQ=255)
                                                                                                                                |                                                                                                                                              
TGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑TAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA  >  NC_002947/1126519‑1126787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑