Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,419,549	+C	intergenic (‑38/+98)	PP_1242 ← / ← PP_1244	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,419,548	1	.	C	100.0%	161.7 / NA	48	intergenic (‑37/+99)	PP_1242/PP_1244	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/25);  total (23/25)

CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGC‑TCTGTGGGTAAATGCG  >  NC_002947/1419401‑1419681                                                                                                                                                      |                                                                                                                                          ctgtgaGGGTATGCCGGGTTGGATGGTGATCAGCGGGTGTGTGTTGTTTTGGGCTTGGTAGATGCAGGTCTTGC‑GGACTGTGGTGGGTTGTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                          <  2:3446/144‑1 (MQ=255) cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                          <  5:284222/149‑1 (MQ=255)                  gTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGcc                                                                                                                         >  3:117300/1‑149 (MQ=255)                   ttCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGccc                                                                                                                        <  6:344785/149‑1 (MQ=255)                    tCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGGCTTGC‑CGACTGTGGTGGGTGCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCg                                                                                                                       <  6:143170/149‑1 (MQ=255)                      gATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCt                                                                                                                     >  2:127162/1‑149 (MQ=255)                       aTGCGGAAAAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTc                                                                                                                    <  2:143458/149‑1 (MQ=255)                         gCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCGCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTcc                                                                                                                   >  1:255002/1‑149 (MQ=255)                          cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTCGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTccc                                                                                                                  <  4:305378/148‑1 (MQ=255)                           ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGACATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCACGGGCACGCCCGCTCCca                                                                                                                 >  8:145429/1‑148 (MQ=255)                                aGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                           <  4:87716/149‑1 (MQ=255)                                 gCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTAc                                                                                                          <  7:185065/149‑1 (MQ=255)                                     gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                      >  2:211560/1‑149 (MQ=255)                                     gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCATGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACtgt                                                                                                       >  4:334295/1‑148 (MQ=255)                                        aGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                          <  6:327331/110‑1 (MQ=255)                                        aGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                          >  5:327331/1‑110 (MQ=255)                                               ttttCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                                            >  7:64031/1‑149 (MQ=255)                                                 ttCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAc                                                                                           <  5:344534/148‑1 (MQ=255)                                                   cGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGCCGGc                                                                                        >  2:206153/1‑149 (MQ=255)                                                    gCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACgg                                                                                         >  8:286674/1‑147 (MQ=255)                                                    gCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGCGCAGTGCGTGACgg                                                                                         <  7:286674/147‑1 (MQ=255)                                                         ggTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt‑g                                                                                   <  1:155078/147‑1 (MQ=255)                                                          gTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgt‑ggt                                                                                 <  8:64031/148‑1 (MQ=255)                                                           tAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGACATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCACGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGa                                                                               <  7:145429/149‑1 (MQ=255)                                                             gAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGAcc                                                                             <  3:334295/149‑1 (MQ=255)                                                                  aGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCtgtg                                                                        <  1:127162/149‑1 (MQ=255)                                                                  aGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGGTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCtgtg                                                                        <  5:289514/149‑1 (MQ=255)                                                                     tcgtGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCGTGGATTCGGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCGGT‑GGTGACCCTGTGGGa                                                                     <  7:248654/146‑1 (MQ=39)                                                                     tCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTgg                                                                       >  1:251886/1‑147 (MQ=255)                                                                     tCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTgg                                                                       >  7:41370/1‑147 (MQ=255)                                                                         gC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTgg                                                                       >  4:272199/1‑143 (MQ=255)                                                                         gC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTgg                                                                       <  3:272199/143‑1 (MQ=255)                                                                          c‑cGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCggg                                                                >  8:111726/1‑149 (MQ=255)                                                                          c‑cGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCgg                                                                 <  2:251886/148‑1 (MQ=255)                                                                          c‑cGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCgg                                                                 <  8:41370/148‑1 (MQ=255)                                                                              aCTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCATTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGt                                                             >  7:307367/1‑149 (MQ=255)                                                                              aCTGTGGTGGGTTCTGTTGTGGTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGt                                                             <  7:111726/149‑1 (MQ=255)                                                                                              ttgtgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCAGGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCt                                            <  2:255002/149‑1 (MQ=255)                                                                                                  gttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCCCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCCGGCGTG‑CCCGCGAAGAGGCCTGAcc                                        >  8:63261/1‑149 (MQ=255)                                                                                                      tgggTGGGCTTTGTTTTGTTGCCCGGTTTGGGGGGGGGCCGGGGCGGGCCT‑CTTCGGGGGCAGGCCCGCTCCCAATGTTAGTGTGCATGGGGTTA‑GGGTGTGGGTCACCCTTTGGTAGGGGGGTTG‑CCCGCGAAGAGGCCTGACCTGc                                     <  8:307367/146‑1 (MQ=255)                                                                                                       cGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTCACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCTGACCTGCTa                                   <  1:211560/149‑1 (MQ=255)                                                                                                         gTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCCCCGGGTCTGGTGAGTGCGTGTCGGGTGT‑GGTGACCCTGTGGGAGCGGGGGTG‑CCCGCGAAGAGGCCGGACCTGCGAAt                                 >  3:312131/1‑149 (MQ=255)                                                                                                              cATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACCCCCCCTCCCACAGGTAAATTGCAGTGCGTTGCGGGTGG‑GGTGACCCTGTGGGAGCCGGCGTG‑CCCGCGAAGAGGCCTGCCCTGCGAATTCaac                            >  2:101472/1‑148 (MQ=25)                                                                                                               aTTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCTGACCTGCTAATGCAAgg                           >  2:69590/1‑149 (MQ=255)                                                                                                                ttgttttgttTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCCCAGGTACTGTGCAGTGCGGGA‑GGCTGG‑GGTGACCCGGTGGGAGCCGGGGTG‑GCCGCGGAGAGGCCTGACCTGCTAATGGAAGgtg                         >  3:238383/1‑149 (MQ=37)                                                                                                                ttgttttgttTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGc                                                               >  3:300713/1‑113 (MQ=255)                                                                                                                ttgttttgttTCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGc                                                               <  4:300713/113‑1 (MQ=255)                                                                                                                   ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                      <  8:103692/72‑1 (MQ=255)                                                                                                                           cccTGGATTCTGGGTTGCCTGGGCTGGCCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGc‑tct               >  1:205169/1‑149 (MQ=255)                                                                                                                             cTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTGTCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGC‑TCtgt             <  1:69590/149‑1 (MQ=255)                                                                                                                             cTGGATTCTGGGTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGGACGGTGCAGGGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCTGGCCTGCTAATGCAAGGTGTGCGGG‑TCtgtg            >  6:274933/1‑149 (MQ=25)                                                                                                                                        gTTGCCTGGGCTGGCCT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGGCTGACCTGCTAATGCAAGGGGTGAGGCGTGGGAGGGTAAATGCg  >  1:287634/1‑149 (MQ=25)                                                                                                                                                      |                                                                                                                                          CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGC‑CGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CT‑CTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGT‑GGTGACCCTGTGGGAGCGGGCGTG‑CCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGC‑TCTGTGGGTAAATGCG  >  NC_002947/1419401‑1419681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑