Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	3 (0.050)	33 (0.580) +CGGG	28/274	1.6	91.9%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	3 (0.050)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGC  >  NC_002947/5681268‑5681557                                                                                                                                                    |                                                                                                                                                   ttCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTGGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCc                                                                                                                                                   >  7:310338‑M1/1‑148 (MQ=255)     cagtaATACATGCAAACACAGACCTCCTGAGGTGGGTTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                               <  8:211534‑M1/147‑6 (MQ=255)                gCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcag                                                                                                                                    >  5:92181‑M1/1‑133 (MQ=255)                 cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagc                                                                                                                                   <  3:288424‑M1/149‑18 (MQ=255)                  aacccctgacctcctGAGGTGGGCTGGGTTACTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGACTTGTGTCGCGAAATGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagcc                                                                                                                                  <  4:265377‑M1/142‑19 (MQ=255)                  aaaCTCAGACCTCCTGAGGTGGACTGGGTTCCTGTTGTGATGCTGATGGCTGCGTTATTTGTTGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagcc                                                                                                                                  <  5:151697‑M1/149‑19 (MQ=255)                  aaaCACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagc                                                                                                                                   >  3:209555‑M1/1‑131 (MQ=255)                       caGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatc                                                                                                                             <  1:146291‑M1/149‑24 (MQ=255)                       caGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTGCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatc                                                                                                                             <  1:73259‑M1/149‑24 (MQ=255)                                  aGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaa                                                                                                                    <  5:2476‑M1/147‑33 (MQ=255)                                  aGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTAGCGAAAGGGGCGCGCAGCGGCCCCAGGCTTTAATTGCCACTGCATGAACCGCcggggctgctgggcaccccgataccgacacaagg                                                                                                                  >  8:88597‑M1/1‑115 (MQ=255)                                    gTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggc                                                                                                                 <  4:209555‑M1/148‑36 (MQ=255)                                              ttCTTGTTGTGATGCTTAGGGCTGGTTTTTTTGTGGGTGCGGCCTTGTGGCGCTAAAGGGGCGCGACGCGGCCCCTGGCTTTCTTTGCCACTGCTTGAACCGCGGGGGCTGGTGCGCAGCCCTATCGCCACACAAGGCCGCTCCCACTc                                                                                                      <  6:92181/149‑1 (MQ=255)                                                 gtggtgtgTTGGTGATGGCTTCGTTCTTTGTGGTAGCGGCTTTGTTGCGCTAACGGGGCGCTAAGGGGCCCCAGGCTTTCATTGCCACTGCATTAACCGCCGGGGCTGCTGCCCAGCCCTATCGCTAAAAAAGGCCGCTCCCACTCCAg                                                                                                   <  6:61654/145‑1 (MQ=255)                                                        gATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCc                                                                                                                                                   <  8:287932‑M1/94‑2 (MQ=255)                                                        gATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCc                                                                                                                                                   >  7:287932‑M1/1‑93 (MQ=255)                                                                                      ggCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagc                                                                                                                                   <  3:80597‑M1/80‑18 (MQ=255)                                                                                      ggCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagc                                                                                                                                   >  4:80597‑M1/1‑63 (MQ=255)                                                                                          ttGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctccccctccagacccccaggccctgcgcctcacatcctaaccaaggtcaggt                                                          >  6:145663‑M1/1‑59 (MQ=255)                                                                                                  cgAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatc                                                  >  4:78832‑M1/1‑51 (MQ=255)                                                                                                                     ccccAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctt                               >  7:41547‑M1/1‑32 (MQ=255)                                                                                                                       ccAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctca                                                                              <  7:283927‑M1/100‑71 (MQ=255)                                                                                                                        cAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttc                             >  3:108472‑M1/1‑29 (MQ=255)                                                                                                                             tttCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgc                       >  7:83707‑M1/1‑24 (MQ=255)                                                                                                                                 aTTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccacgccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgac                    >  6:144462‑M1/1‑20 (MQ=255)                                                                                                                                 aTTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgaaacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgacc                   <  8:238494‑M1/149‑130 (MQ=255)                                                                                                                                  ttGCCACTGCATGAACCGCcggggctgctgcgccgccctatcgcgacacaaggccgctcccactccagaccaccaggcccagcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgac                    >  1:261153‑M1/1‑19 (MQ=255)                                                                                                                                  ttGCCACTGCATGAACCGCCGGGGCTGCTGGGCCGCCCGAGAGCGACACAAGGCCGCGCCCCCTCCAGACCCCCCGGCCCGGCGCCTCCCCTCCGAACCACGGGCAGGAGGCAGATCCGCGTCACGCCCCAGCCGTTCACATGCGAc                    >  2:224227/1‑147 (MQ=255)                                                                                                                                     ccACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggt               >  7:156‑M1/1‑16 (MQ=255)                                                                                                                                       aCTGCATGAACCGCcggggctgctgcgcagccctaacgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtcc             >  5:139860‑M1/1‑14 (MQ=255)                                                                                                                                        cTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgctccagcctttccaatgcgaccaggtccg            <  3:316432‑M1/149‑137 (MQ=255)                                                                                                                                        cTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccg            <  3:78832‑M1/149‑137 (MQ=255)                                                                                                                                           cATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtcc             <  7:154946‑M1/145‑136 (MQ=255)                                                                                                                                           cATGAACCGCcggggctgctgcgcagccctatcgcgacacaacgccgctcccccaccagaccacccggcccagcgcctcacaaacaaaccaaagtcaggttgcagatccgcctcacgccccagcctttccaaagcgaccaggtcccag          >  8:154946‑M1/1‑10 (MQ=255)                                                                                                                                            aTGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcggctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtac        >  1:58113‑M1/1‑9 (MQ=255)                                                                                                                                             tGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacc       >  1:66965‑M1/1‑8 (MQ=255)                                                                                                                                              gAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcccgcaccagcctttccactgcgcccaggtccggtaccc      >  6:233923‑M1/1‑7 (MQ=255)                                                                                                                                                 ccgccggggctgctgcgcagccctttcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctca                                                                              <  2:23504‑M1/74‑71 (MQ=255)                                                                                                                                                  cgccggggctgctgcgcagccctatcgccacacatggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacccgcgc  <  7:88597‑M1/149‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGC  >  NC_002947/5681268‑5681557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.