Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,257	A→C	intergenic (+494/+259)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,257	0	A	C	100.0%	161.1 / NA	46	intergenic (+494/+259)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/21);  total (25/21)

TCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTC  >  NC_002947/4741122‑4741392                                                                                                                                        |                                                                                                                                          tCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGcccc                                                                                                                              <  8:6118/149‑1 (MQ=255)   cGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCg                                                                                                                             <  6:166267/148‑1 (MQ=255)                 tCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                                               >  6:265993/1‑146 (MQ=255)                   gTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgcag                                                                                                            >  3:130460/1‑144 (MQ=255)                                       aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgcg                                                                                       >  1:285324/1‑149 (MQ=255)                                       aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgc                                                                                        <  2:241061/148‑1 (MQ=255)                                         gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgcggc                                                                                     <  4:130460/149‑1 (MQ=255)                                            gcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGcc                                                                                  <  3:290203/149‑1 (MQ=255)                                               tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCtt                                                                                >  6:329156/1‑148 (MQ=255)                                                cACCGGCCATGGCGTATTGAACTGGGTATCGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGa                                                                              <  6:177280/149‑1 (MQ=255)                                                 aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGAt                                                                             >  8:216607/1‑149 (MQ=255)                                                         tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTca                                                                     >  1:334493/1‑149 (MQ=255)                                                            cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCacac                                                                  <  8:2970/149‑1 (MQ=255)                                                                   aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                             >  5:295004/1‑147 (MQ=255)                                                                   aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                             >  7:159484/1‑147 (MQ=255)                                                                   aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                           <  6:206301/149‑1 (MQ=255)                                                                   aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGAGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                             >  1:103028/1‑147 (MQ=255)                                                                        ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTTAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCa                                                                           <  2:290608/128‑1 (MQ=255)                                                                        ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCa                                                                           >  1:290608/1‑128 (MQ=255)                                                                        ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                        <  2:334493/147‑1 (MQ=255)                                                                        ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                      >  4:72885/1‑149 (MQ=255)                                                                        ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                      >  5:168788/1‑149 (MQ=255)                                                                         gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                     <  3:289416/149‑1 (MQ=255)                                                                         gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                     <  7:19649/149‑1 (MQ=255)                                                                          tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGCCACGTCCTCCTTCAGCGCCTCGACCT‑TGGCCCGAGGCGCCTTGATCACGTTCAAACCCAGATTGGCCTTg                                                    >  8:267416/1‑149 (MQ=255)                                                                             aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTAc                                                 >  6:10950/1‑149 (MQ=255)                                                                               gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGCCACGTCCTCCTTCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACCCCGCGATAGGCCTTGTACtt                                               >  8:241216/1‑149 (MQ=255)                                                                               gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                               <  5:329156/149‑1 (MQ=255)                                                                                tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                   >  8:13067/1‑144 (MQ=255)                                                                                tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                   <  7:13067/144‑1 (MQ=255)                                                                                tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                              <  2:119268/149‑1 (MQ=255)                                                                                 ccAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTgg                                             >  8:15686/1‑149 (MQ=255)                                                                                             aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc                                  <  3:27926/148‑1 (MQ=255)                                                                                               cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCCCACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                               >  3:272077/1‑149 (MQ=255)                                                                                               cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACCCCGCGCTAGGCCTTGTACTTGGCCCCGGCCACCTCa                               >  2:226199/1‑149 (MQ=255)                                                                                               cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                               >  5:260885/1‑149 (MQ=255)                                                                                                ttCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                              >  7:96480/1‑149 (MQ=255)                                                                                                                       acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc       <  5:314817/143‑1 (MQ=255)                                                                                                                       acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc       <  2:339799/143‑1 (MQ=255)                                                                                                                       acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc       <  5:10950/143‑1 (MQ=255)                                                                                                                         ttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc     >  5:97752/5‑149 (MQ=255)                                                                                                                            caTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc  <  3:72885/148‑1 (MQ=255)                                                                                                                            caTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCAGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc  >  5:121443/2‑149 (MQ=255)                                                                                                                             aTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc  >  1:241468/1‑149 (MQ=255)                                                                                                                             aTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                         <  6:266628/125‑1 (MQ=255)                                                                                                                             aTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                         >  5:266628/1‑125 (MQ=255)                                                                                                                                        |                                                                                                                                          TCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTC  >  NC_002947/4741122‑4741392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑