Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,741,294 C→G intergenic (+531/+222) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,741,2940CG97.9% 162.2 / ‑4.1 48intergenic (+531/+222)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (24/23);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

ACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTT  >  NC_002947/4741160‑4741412
                                                                                                                                       |                                                                                                                       
aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgcg                                                                                                           >  1:285324/1‑149 (MQ=255)
aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgc                                                                                                            <  2:241061/148‑1 (MQ=255)
  gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgcggc                                                                                                         <  4:130460/149‑1 (MQ=255)
     gcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGcc                                                                                                      <  3:290203/149‑1 (MQ=255)
        tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCtt                                                                                                    >  6:329156/1‑148 (MQ=255)
         cACCGGCCATGGCGTATTGAACTGGGTATCGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGa                                                                                                  <  6:177280/149‑1 (MQ=255)
          aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGAt                                                                                                 >  8:216607/1‑149 (MQ=255)
                  tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTca                                                                                         >  1:334493/1‑149 (MQ=255)
                     cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCacac                                                                                      <  8:2970/149‑1 (MQ=255)
                            aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                 >  7:159484/1‑147 (MQ=255)
                            aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                 >  5:295004/1‑147 (MQ=255)
                            aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                               <  6:206301/149‑1 (MQ=255)
                            aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGAGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                 >  1:103028/1‑147 (MQ=255)
                                 ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTTAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCa                                                                                               <  2:290608/128‑1 (MQ=255)
                                 ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCa                                                                                               >  1:290608/1‑128 (MQ=255)
                                 ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                            <  2:334493/147‑1 (MQ=255)
                                 ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                          >  5:168788/1‑149 (MQ=255)
                                 ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                          >  4:72885/1‑149 (MQ=255)
                                  gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                                         <  3:289416/149‑1 (MQ=255)
                                  gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                                         <  7:19649/149‑1 (MQ=255)
                                   tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGCCACGTCCTCCTTCAGCGCCTCGACCT‑TGGCCCGAGGCGCCTTGATCACGTTCAAACCCAGATTGGCCTTg                                                                        >  8:267416/1‑149 (MQ=255)
                                      aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTAc                                                                     >  6:10950/1‑149 (MQ=255)
                                        gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGCCACGTCCTCCTTCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACCCCGCGATAGGCCTTGTACtt                                                                   >  8:241216/1‑149 (MQ=255)
                                        gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                   <  5:329156/149‑1 (MQ=255)
                                         tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                                       >  8:13067/1‑144 (MQ=255)
                                         tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                                       <  7:13067/144‑1 (MQ=255)
                                         tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                  <  2:119268/149‑1 (MQ=255)
                                          ccAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTgg                                                                 >  8:15686/1‑149 (MQ=255)
                                                      aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc                                                      <  3:27926/148‑1 (MQ=255)
                                                        cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCCCACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                                                   >  3:272077/1‑149 (MQ=255)
                                                        cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACCCCGCGCTAGGCCTTGTACTTGGCCCCGGCCACCTCa                                                   >  2:226199/1‑149 (MQ=255)
                                                        cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                                                   >  5:260885/1‑149 (MQ=255)
                                                         ttCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                                                  >  7:96480/1‑149 (MQ=255)
                                                                                acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc                           <  5:10950/143‑1 (MQ=255)
                                                                                acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc                           <  5:314817/143‑1 (MQ=255)
                                                                                acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc                           <  2:339799/143‑1 (MQ=255)
                                                                                  ttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                         >  5:97752/5‑149 (MQ=255)
                                                                                     caTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc                      <  3:72885/148‑1 (MQ=255)
                                                                                     caTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCAGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc                      >  5:121443/2‑149 (MQ=255)
                                                                                      aTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc                      >  1:241468/1‑149 (MQ=255)
                                                                                      aTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                                             >  5:266628/1‑125 (MQ=255)
                                                                                      aTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                                             <  6:266628/125‑1 (MQ=255)
                                                                                                  aggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGc         >  8:281368/7‑149 (MQ=255)
                                                                                                    gcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCa       <  7:281368/145‑1 (MQ=255)
                                                                                                      acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGt     <  8:96480/147‑1 (MQ=255)
                                                                                                       caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTg                                    >  4:206840/2‑117 (MQ=255)
                                                                                                       caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTg                                    <  3:206840/116‑1 (MQ=255)
                                                                                                         ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGtttt  <  7:216607/149‑1 (MQ=255)
                                                                                                                                       |                                                                                                                       
ACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTT  >  NC_002947/4741160‑4741412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑