Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,499,4971.C83.8% 74.6 / 4.1 38intergenic (+74/‑90)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (10/21);  minor base A (6/0);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 3.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0029471,499,4980TG100.0% 108.2 / NA 38intergenic (+75/‑89)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/22);  total (16/22)

ACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG  >  NC_002947/1499356‑1499644
                                                                                                                                                ||                                                                                                                                                    
aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAAAACGCGCCGCGGGGCCTGGCACCCGCGTTGCCCGTGt‑t‑tgccggc                                                                                                                                                 >  3:291614/1‑143 (MQ=255)
       tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCCGTGT‑TAGCCGGCAcg‑c‑ccc                                                                                                                                         >  8:256634/1‑147 (MQ=37)
            cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTGT‑TAGCGGGCAcg‑c‑ccgcgccc                                                                                                                                    >  2:206569/1‑142 (MQ=25)
                  aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGa                                                                                                                               <  3:291243/148‑1 (MQ=21)
                         cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCTCCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGc                                                                                                                       <  5:311871/149‑1 (MQ=25)
                              agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACCCGCGTTGCCGGTGT‑TAGCGGGCACG‑CTC‑GCTCCCACAGACTGAATGCCAtt                                                                                                                   >  2:44851/1‑148 (MQ=25)
                               gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTaa                                                                                                                 <  1:267108/149‑1 (MQ=21)
                                 gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACACGCGCCGCGGGGCATGGCACCCGCGTTTCCGGTGT‑TAGCGGGCACGCC‑C‑CCGCCCCCCCACTGATTGCCAATAAGc                                                                                                               >  4:117138/1‑149 (MQ=255)
                                 gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGc                                                                                                               >  5:144613/1‑149 (MQ=21)
                                   cttaaCGAAGCCTTGGCACCACAGCTGGATTGAGGCTGCCAATCGTTGGGAGCGGGCGTGCCCGCTAAACTGGCGCCGCGGGGCATGTCACCGGATTTGCCGGTGT‑TCGCGGTCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                             <  4:250370/146‑1 (MQ=18)
                                   cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                             <  1:206569/149‑1 (MQ=21)
                                      aaCGAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGGGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGCCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                                           <  5:19766/148‑1 (MQ=25)
                                      aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCCGGCCTGCCCCCGCAACAGGCCCCGCGGTGCATGGCACCGGCATTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACAGAATGCCATTAAGCggggc                                                                                                          >  5:167496/1‑147 (MQ=21)
                                                     ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                                                           <  6:103222/149‑1 (MQ=21)
                                                      cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg                                                                                          >  7:221092/1‑149 (MQ=21)
                                                        cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc                                                                                        <  5:306495/149‑1 (MQ=21)
                                                          gcagGCCTGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGAAACACGCGCCCAGCGGCATGGCACCGGCAGAGCCGGTGT‑TAGCGGGCACGCC‑C‑GCTCCCACACGCTGATTGCCAATTAGAGGGGCTGTCGCAACAGGGTCGGgcc                                                                                      >  2:256758/1‑149 (MQ=255)
                                                                 tGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCCCGGTGCATGGCACCCGCGTTGCCGGTGT‑TAGCCGGCCCGCC‑C‑GCGCCCCCCGACTGAATGCCATTAAGCCGGGCGATCGGTACAGTGGCGGCCCGgttttt                                                                               >  3:283999/1‑149 (MQ=16)
                                                                   aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                               <  6:144613/147‑1 (MQ=21)
                                                                   aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                               <  8:221092/147‑1 (MQ=21)
                                                                     gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                            <  4:291614/148‑1 (MQ=21)
                                                                      cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                                                          <  3:117138/149‑1 (MQ=21)
                                                                                        gggcgggcCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGGTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                        <  4:43709/149‑1 (MQ=37)
                                                                                           cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACG‑CTC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc                                                     <  1:44851/149‑1 (MQ=37)
                                                                                               cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                                                 <  7:232972/149‑1 (MQ=37)
                                                                                                ccGCGAAACAGGCGCCGCGGTGCTTGGCACCGGCTTGGCCGGTGTGC‑GCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTAGCCTCGCTAACGAATCATGACTCCCTTa                                                <  1:193295/149‑1 (MQ=25)
                                                                                                ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTAACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                <  5:280091/149‑1 (MQ=37)
                                                                                                 cgcgAAACAGGCGCCGCGGTGCATGGTACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCGTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                                                     <  7:265414/127‑1 (MQ=25)
                                                                                                 cgcgAAACAGGCGCCGCGGTGCATGGTACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                                                     >  8:265414/1‑127 (MQ=25)
                                                                                                  gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg                                               >  6:2110/1‑148 (MQ=37)
                                                                                                   ctaaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                                             <  1:321244/147‑1 (MQ=37)
                                                                                                         aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                       <  5:2110/149‑1 (MQ=37)
                                                                                                                       aTGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                            <  3:17363/98‑1 (MQ=21)
                                                                                                                       aTGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                            >  4:17363/1‑98 (MQ=21)
                                                                                                                       aTGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACG‑C‑CAGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa                          >  2:214489/1‑148 (MQ=38)
                                                                                                                          gcaccGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCCATc                                      >  7:163753/5‑133 (MQ=37)
                                                                                                                          gcaccGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCCATc                                      <  8:163753/129‑1 (MQ=37)
                                                                                                                            accGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc                    >  2:8424/3‑149 (MQ=38)
                                                                                                                               ggCTTTGCCGGTGT‑TCGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc                  >  5:77251/1‑148 (MQ=39)
                                                                                                                                             ttcgcGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa    <  7:256634/145‑1 (MQ=255)
                                                                                                                                               cgcGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg  >  4:251195/3‑149 (MQ=255)
                                                                                                                                               cgcGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc   <  1:8424/146‑1 (MQ=255)
                                                                                                                                                ||                                                                                                                                                    
ACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG  >  NC_002947/1499356‑1499644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑