mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-59_S59_L001_R1_001.good.fq | 341,962 | 48,650,912 | 100.0% | 142.3 bases | 149 bases | 95.3% |
| errors | ALE-59_S59_L001_R2_001.good.fq | 341,962 | 48,468,656 | 100.0% | 141.7 bases | 149 bases | 95.8% |
| errors | ALE-59_S59_L002_R1_001.good.fq | 335,881 | 47,685,567 | 100.0% | 142.0 bases | 149 bases | 96.7% |
| errors | ALE-59_S59_L002_R2_001.good.fq | 335,881 | 47,554,529 | 100.0% | 141.6 bases | 149 bases | 96.3% |
| errors | ALE-59_S59_L003_R1_001.good.fq | 345,124 | 48,933,009 | 100.0% | 141.8 bases | 149 bases | 96.6% |
| errors | ALE-59_S59_L003_R2_001.good.fq | 345,124 | 48,771,325 | 100.0% | 141.3 bases | 149 bases | 96.9% |
| errors | ALE-59_S59_L004_R1_001.good.fq | 321,711 | 45,624,211 | 100.0% | 141.8 bases | 149 bases | 96.6% |
| errors | ALE-59_S59_L004_R2_001.good.fq | 321,711 | 45,498,661 | 100.0% | 141.4 bases | 149 bases | 96.0% |
| total | 2,689,356 | 381,186,870 | 100.0% | 141.7 bases | 149 bases | 96.3% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 59.7 | 2.2 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 37611 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 126 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.006 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.82557 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 00:27:23 19 Feb 2020 | 00:28:03 19 Feb 2020 | 40 seconds |
| Read alignment to reference genome | 00:28:04 19 Feb 2020 | 00:33:45 19 Feb 2020 | 5 minutes 41 seconds |
| Preprocessing alignments for candidate junction identification | 00:33:45 19 Feb 2020 | 00:34:23 19 Feb 2020 | 38 seconds |
| Preliminary analysis of coverage distribution | 00:34:23 19 Feb 2020 | 00:35:57 19 Feb 2020 | 1 minute 34 seconds |
| Identifying junction candidates | 00:35:57 19 Feb 2020 | 00:38:17 19 Feb 2020 | 2 minutes 20 seconds |
| Re-alignment to junction candidates | 00:38:17 19 Feb 2020 | 00:39:57 19 Feb 2020 | 1 minute 40 seconds |
| Resolving best read alignments | 00:39:57 19 Feb 2020 | 00:40:56 19 Feb 2020 | 59 seconds |
| Creating BAM files | 00:40:56 19 Feb 2020 | 00:42:23 19 Feb 2020 | 1 minute 27 seconds |
| Tabulating error counts | 00:42:23 19 Feb 2020 | 00:42:51 19 Feb 2020 | 28 seconds |
| Re-calibrating base error rates | 00:42:51 19 Feb 2020 | 00:42:54 19 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 00:42:54 19 Feb 2020 | 00:48:24 19 Feb 2020 | 5 minutes 30 seconds |
| Polymorphism statistics | 00:48:24 19 Feb 2020 | 00:48:24 19 Feb 2020 | 0 seconds |
| Output | 00:48:24 19 Feb 2020 | 00:48:44 19 Feb 2020 | 20 seconds |
| Total | 21 minutes 20 seconds | ||
