Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	77.5 / NA	30	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/21);  total (9/21)

CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG  >  NC_002947/1499360‑1499649                                                                                                                                                      |                                                                                                                                                  cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑cg‑‑‑c                                                                                                                                                <  6:143415/149‑1 (MQ=37)          cggTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGGGGGCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCca                                                                                                                                        <  5:3116/147‑1 (MQ=21)           cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCcac                                                                                                                                       <  5:123918/149‑1 (MQ=25)                   ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGACCGCGAAACAGGCGCCGCCGTGCATGGCACCGGCGTTGCCGGTGTTAGCGGGCA‑CGC‑‑CCGCTCCCACACACTGAt                                                                                                                               >  3:347383/1‑149 (MQ=255)                           agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCAtt                                                                                                                        >  5:179756/1‑148 (MQ=25)                              gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGc                                                                                                                    <  7:5123/149‑1 (MQ=21)                                cTGAACGACGCCTTGCCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGGGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACGGGCTTTGCGGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGa                                                                                                                                    <  1:369487/131‑1 (MQ=18)                                cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                                  <  7:350340/149‑1 (MQ=21)                                 ttatttaaGCTTTGGCACCACAGCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGTTCGCGGGCA‑GGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGc                                                                                                                    <  8:169742/140‑1 (MQ=18)                                      gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGGGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAACCGGGCCTAt                                                                                                            <  3:337228/149‑1 (MQ=21)                                                  ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACCGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                                                                 <  1:315619/149‑1 (MQ=21)                                                  ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                                                                <  1:63180/149‑1 (MQ=21)                                                                  gcggCCAAACGGGGGGCGCGGGGGGGCCCGCGAAACTGGCGCCGCGGGGCAGGCCCCCGGCTTTGCGGGGGTG‑CGGGGGC‑CGCCCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                                 <  1:334998/146‑1 (MQ=255)                                                                  gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCA‑CGC‑‑CCGCTCCCACAGCCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg                                                                                >  5:412635/1‑149 (MQ=21)                                                                      ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                                                         <  8:230629/120‑1 (MQ=17)                                                                      ccAAACGGGGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc                                                                              <  2:244194/147‑1 (MQ=21)                                                                          aCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                                                                        <  1:330003/149‑1 (MQ=21)                                                                          aCGGTGGGAGCCGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt                                                                         >  3:302048/1‑148 (MQ=21)                                                                                   gCGGGCGTGCCCGCTAAACCGGCGCCGCGGTCAATGGCACGGGCTTTGCCGGTGTTCGCGGGAA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                               <  3:33342/149‑1 (MQ=25)                                                                                     gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                             >  6:6373/1‑149 (MQ=25)                                                                                            cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                                                      <  7:131089/149‑1 (MQ=37)                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                                                                  <  3:133517/136‑1 (MQ=25)                                                                                             ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                                                                  >  4:133517/1‑136 (MQ=25)                                                                                              cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                       >  7:164368/1‑146 (MQ=37)                                                                                               gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGATACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa                                                   <  1:109485/149‑1 (MQ=37)                                                                                                    aCAGGCCCCGCGGGGCATGGCACCGGCTTTGCCGGTGTTCGCGGCCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt                                              <  6:241116/149‑1 (MQ=37)                                                                                                     cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                             <  6:179756/149‑1 (MQ=37)                                                                                                      aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                            >  6:95521/1‑149 (MQ=37)                                                                                                      aGGCGACTCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                            <  7:19295/149‑1 (MQ=37)                                                                                                                                              gggCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcgg    >  6:127648/1‑149 (MQ=255)                                                                                                                                                gCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg  <  7:308171/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG  >  NC_002947/1499360‑1499649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑