Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	307,866	+C	intergenic (+881/‑988)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	226.1 / NA	66	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (33/33);  total (33/33)

AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307718‑308008                                                                                                                                                      |                                                                                                                                                 aaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCATGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑c                                                                                                                                                 >  4:49690/1‑149 (MQ=255)  aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑cc                                                                                                                                                <  1:139380/149‑2 (MQ=255)  aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑cc                                                                                                                                                <  6:233277/149‑2 (MQ=255)  aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCAGATGTACTGCCTATGCACTGCGCGGCCAAAATCGGCGAAGAAGTCGATGTGACATTGTTCTTCGGCCTGTCCGGCAATGGCAAGA‑CTAACCTTTCGGCTGACGAAAG‑cc                                                                                                                                                <  2:301157/149‑2 (MQ=255)    ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                              >  1:388100/1‑146 (MQ=255)     cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑ccgtt                                                                                                                                             <  3:234548/149‑5 (MQ=255)                tGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGt                                                                                                                                   <  6:243668/149‑1 (MQ=255)                tGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGt                                                                                                                                   >  7:315049/1‑149 (MQ=255)                  cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTga                                                                                                                                 <  2:247591/149‑1 (MQ=255)                        ttCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTgacgac                                                                                                                             >  5:24141/1‑147 (MQ=255)                           ctgctgCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGc                                                                                                                         <  3:156584/148‑1 (MQ=255)                           ctgctgCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCa                                                                                                                        <  5:10879/149‑1 (MQ=255)                            tgctgcCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCAc                                                                                                                       >  6:74930/1‑149 (MQ=255)                            tgctgcCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTATCGGCTCACGAAAGCCGTTACCTGATCGGTGACGACGAGCAc                                                                                                                       >  6:315485/1‑149 (MQ=255)                              ctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACg                                                                                                                      <  3:392796/148‑1 (MQ=255)                                gccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                                   >  6:412448/1‑149 (MQ=255)                                 ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGCCGAAAGCCGTTACCTGATCGGTGACGAGGAGCACGGGTg                                                                                                                  >  8:374674/1‑149 (MQ=255)                                 ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                                   <  3:390206/148‑1 (MQ=255)                                    gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                               <  7:213911/149‑1 (MQ=255)                                    gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                               >  4:164340/1‑149 (MQ=255)                                     ctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCTTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTTGGGc                                                                                                              <  3:49690/149‑1 (MQ=255)                                        ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGaa                                                                                                           <  8:315049/149‑1 (MQ=255)                                           aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc                                                                                                        >  5:406980/1‑149 (MQ=255)                                              tACTGCCTATGCACTGCGTGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                     <  6:351905/149‑1 (MQ=255)                                               aCTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTCGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCAGATAGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTg                                                                                                    >  1:269528/1‑149 (MQ=255)                                                cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                                   >  3:403762/1‑149 (MQ=255)                                                  gCCTATGCACTGCACGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                                 >  3:199590/1‑149 (MQ=255)                                                    cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑c                                                                                              <  2:126219/149‑1 (MQ=255)                                                     tATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCAGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGTGGCGAAGGCGTTGTCTT‑c                                                                                              >  3:332902/1‑148 (MQ=255)                                                       tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑Caa                                                                                            >  5:211038/1‑148 (MQ=255)                                                          aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATg                                                                                        <  3:253712/149‑1 (MQ=255)                                                          aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATg                                                                                        >  3:197499/1‑149 (MQ=255)                                                              cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGaa                                                                                     <  6:24141/148‑1 (MQ=255)                                                                 ggCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAggcg                                                                                 >  7:28315/1‑149 (MQ=255)                                                                   ccAACATCGGCGAAGAAGGCGATGTGACCTTGTGCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAggcggc                                                                               <  2:107112/149‑1 (MQ=255)                                                                    cAACATCTGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGgct                                                                              <  7:148353/149‑1 (MQ=255)                                                                        aTCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑Caa                                                                                            >  7:45220/1‑131 (MQ=255)                                                                        aTCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑Caa                                                                                            <  8:45220/131‑1 (MQ=255)                                                                         tCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTAc                                                                         <  7:276346/149‑1 (MQ=255)                                                                         tCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTAc                                                                         <  7:184177/149‑1 (MQ=255)                                                                         tCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTAc                                                                         <  5:74930/149‑1 (MQ=255)                                                                         tCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTAc                                                                         <  4:199590/149‑1 (MQ=255)                                                                           ggCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGc                                                                       >  4:367620/1‑149 (MQ=255)                                                                           ggCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCAGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGc                                                                       >  7:164065/1‑149 (MQ=255)                                                                            gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGcc                                                                      <  8:164065/149‑1 (MQ=255)                                                                                agaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGt                                                                  <  6:211038/149‑1 (MQ=255)                                                                                 gaagGCGATCTGACCTTGTTCTTCGGCCTGTCCGGCACAGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTCATCGGTGACGACGAGCACCGTTGCGGCGAAGGCGTTGTCTT‑CAACATCCAAGCCGGCTGCTACGCCAAGTg                                                                 >  6:135433/1‑149 (MQ=255)                                                                                    ggCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCAt                                                              <  7:374674/149‑1 (MQ=255)                                                                                    ggCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCAt                                                              <  4:197499/149‑1 (MQ=255)                                                                                       gATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa                                                           >  7:83084/1‑149 (MQ=255)                                                                                        aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                          <  8:28315/149‑1 (MQ=255)                                                                                          gtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGGGACGACGAGCACGGTTGGGGCGAAGGCGTTGGCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCTTCGACCt                                                        >  6:413522/1‑149 (MQ=255)                                                                                            gACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt                                                      >  5:339451/1‑149 (MQ=255)                                                                                                  gTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAg                                                                   <  1:422031/130‑1 (MQ=255)                                                                                                  gTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAg                                                                   >  2:422031/1‑130 (MQ=255)                                                                                                     cttcGGCCTGTCCGGCACTGGCAAGATCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                               >  1:5838/1‑85 (MQ=255)                                                                                                     cttcGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggt                                                                                                               <  2:5838/84‑2 (MQ=255)                                                                                                        cGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                           <  3:164340/148‑1 (MQ=255)                                                                                                           ccTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc                                       <  4:169625/149‑1 (MQ=255)                                                                                                           ccTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc                                       <  6:406980/149‑1 (MQ=255)                                                                                                                  ggCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCAt                                >  6:254565/1‑149 (MQ=255)                                                                                                                     aCTGGCAAGA‑CTACCCTTTCGGCTGACGATAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGTGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                             <  2:269528/149‑1 (MQ=255)                                                                                                                           aaGA‑CTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGGCCGAGAAGAACGAGCCGGTCATCTGGAAAGc                       >  3:250449/1‑149 (MQ=255)                                                                                                                                cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   <  4:250449/149‑1 (MQ=255)                                                                                                                                 tACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   >  2:280800/1‑148 (MQ=255)                                                                                                                                      tttCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc             >  6:91256/1‑149 (MQ=255)                                                                                                                                             tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTAGAACATTGAAGGCGGCTGCAACCCCAAGTGCATAGACCTGTCCGAAAAGAACGAGCCGGTGATAGTGAAAGCCATAAAGCCCcgcg         >  1:120172/1‑147 (MQ=255)                                                                                                                                             tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc             <  5:304518/142‑1 (MQ=255)                                                                                                                                             tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc             >  6:304518/1‑142 (MQ=255)                                                                                                                                             tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg      >  5:228480/1‑149 (MQ=255)                                                                                                                                                cGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGACAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAc   >  5:34549/1‑149 (MQ=255)                                                                                                                                                 gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  >  4:363447/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                 AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGA‑CTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTT‑CAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307718‑308008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑