Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7501.G98.5% 218.6 / ‑3.7 65intergenic (+112/+28)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (34/30);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674610‑5674890
                                                                                                                                             |                                                                                                                                              
gcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccccct                                                                                                                                        >  4:197309/1‑142 (MQ=255)
  ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTAGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCg                                                                                                                                      >  4:320589/1‑149 (MQ=255)
           ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGc                                                                                                                             <  6:101684/149‑1 (MQ=255)
             gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                           >  7:191770/1‑149 (MQ=255)
              aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCGCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACTGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCt                                                                                                                            <  5:97504/147‑1 (MQ=37)
              aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTgg                                                                                                                          >  2:175920/1‑149 (MQ=255)
                ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTgg                                                                                                                          >  4:145911/1‑147 (MQ=255)
                ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTgg                                                                                                                          >  7:267215/1‑147 (MQ=255)
                 cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAg                                                                                                                       <  7:57803/149‑1 (MQ=255)
                       ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTa                                                                                                                 <  1:175920/149‑1 (MQ=255)
                              aGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                    >  3:386950/1‑139 (MQ=255)
                              aGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                    <  4:386950/139‑1 (MQ=255)
                               gAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTAGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGc                                                                                                         <  3:320589/148‑1 (MQ=255)
                                  gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTc                                                                                                      >  4:239/1‑148 (MQ=255)
                                     ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTcac                                                                                                    <  3:195904/147‑1 (MQ=255)
                                      cGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACAcc                                                                                                 >  3:325121/1‑149 (MQ=255)
                                      cGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACAcc                                                                                                 >  3:319580/1‑149 (MQ=255)
                                        aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCCGCTGGCAGAAATTAC‑TTCTGCTTCACACCtt                                                                                               >  1:66908/1‑149 (MQ=255)
                                        aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCtt                                                                                               >  1:60917/1‑149 (MQ=255)
                                         gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTc                                                                                              <  4:126875/149‑1 (MQ=255)
                                            ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGa                                                                                            >  8:232952/1‑148 (MQ=255)
                                               tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTcaca                                                                                                   >  3:290375/1‑138 (MQ=255)
                                               tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTcaca                                                                                                   <  4:290375/138‑1 (MQ=255)
                                                 ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTg                                                                                       <  3:145911/148‑1 (MQ=255)
                                                  ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGa                                                                                      >  3:188464/1‑148 (MQ=255)
                                                    cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGTATTTCTTGATCTCTACTGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTGGGTTTTCAGCTGGCAGAAATTACTTTCTTCTTCACACCTTCGACACTGat                                                                                     <  1:4475/148‑1 (MQ=37)
                                                      cAGCCCTATCAAGACACAAGGCAGCTCCTGCAGAGAATTTCTTGATCTATACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAATCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCAAACGTTCGACACTGATAtc                                                                                  >  2:391125/1‑148 (MQ=37)
                                                         cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATAtccagg                                                                              <  3:197309/149‑1 (MQ=255)
                                                          ccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                             <  1:286701/149‑1 (MQ=255)
                                                           cTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACGGATAtccaggtc                                                                            >  4:291768/1‑149 (MQ=255)
                                                                  gACACAAGGCCGCTCCTGCAGAGAAGTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGCTAGGGCCGCAAAGCGG‑CCGCCTCGGTGTTCAGCTGGCAGAAGTTGC‑TTCGGCGGCGCGCCTTCGCCGCGGATATCCAGGTCCAGGGTc                                                                     >  8:129432/1‑149 (MQ=25)
                                                                    cacaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTg                                                                   >  6:171430/1‑149 (MQ=255)
                                                                           ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                            <  8:191770/149‑1 (MQ=255)
                                                                            cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                           <  3:289950/149‑1 (MQ=255)
                                                                            cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CACCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTAGACACTGAGATCCAGGTCCAGGTTCTGCGAAGTAg                                                           >  2:175137/1‑149 (MQ=39)
                                                                               tCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                        <  2:149904/133‑1 (MQ=255)
                                                                                   gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                    >  7:347438/1‑149 (MQ=255)
                                                                                    cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                    >  3:37371/1‑132 (MQ=255)
                                                                                    cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                    <  4:37371/132‑1 (MQ=255)
                                                                                      gagaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGcc                                                 <  5:326395/149‑1 (MQ=255)
                                                                                        gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                               <  8:277043/149‑1 (MQ=255)
                                                                                             tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                           <  7:129432/148‑1 (MQ=255)
                                                                                             tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATg                                          >  2:66098/1‑149 (MQ=255)
                                                                                              cTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc                                         >  6:100375/1‑149 (MQ=255)
                                                                                                      ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                                 >  6:369147/1‑149 (MQ=255)
                                                                                                       tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                    <  8:347438/145‑1 (MQ=255)
                                                                                                        aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               >  4:14041/1‑149 (MQ=255)
                                                                                                         cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               >  5:410836/1‑148 (MQ=255)
                                                                                                               cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttc                        <  7:232952/149‑1 (MQ=255)
                                                                                                                  ccTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc                     >  2:254770/1‑149 (MQ=255)
                                                                                                                    ttGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                   >  7:373869/1‑149 (MQ=255)
                                                                                                                    ttGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                   >  4:158614/1‑149 (MQ=255)
                                                                                                                       tgtCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt                <  3:239/149‑1 (MQ=255)
                                                                                                                         tCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg              >  7:85512/1‑149 (MQ=255)
                                                                                                                           gTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg            <  3:158614/149‑1 (MQ=255)
                                                                                                                                aGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                           <  5:203358/129‑1 (MQ=255)
                                                                                                                                aGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                           >  6:203358/1‑129 (MQ=255)
                                                                                                                                 gtgCCGCAAAGA‑GTCCCCATCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCAAACCTTCGACACTGATATCCAGGTCCAGGTTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                  <  7:233037/135‑1 (MQ=255)
                                                                                                                                 gggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt       <  5:100375/148‑1 (MQ=255)
                                                                                                                                 gggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      <  1:66098/149‑1 (MQ=255)
                                                                                                                                 gggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      <  1:254770/149‑1 (MQ=255)
                                                                                                                                   gacGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  4:319580/147‑1 (MQ=255)
                                                                                                                                   gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  4:325121/149‑1 (MQ=255)
                                                                                                                                    ccGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg   >  6:67752/1‑149 (MQ=255)
                                                                                                                                     cGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  3:50838/1‑149 (MQ=255)
                                                                                                                                     cGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  3:396285/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTAC‑TTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674610‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑