Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947196,3510CG27.5% 214.9 / 91.2 131T619T (ACC→ACG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (46/49);  new base G (11/25);  total (57/74)
Fisher's exact test for biased strand distribution p-value = 7.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 133 total aligned reads displayed.

CCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCC  >  NC_002947/196206‑196497
                                                                                                                                                  |                                                                                                                                                   
ccACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAt                                                                                                                                                   >  7:89647/1‑148 (MQ=39)
 cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTa                                                                                                                                                 >  7:38966/1‑149 (MQ=39)
 cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTa                                                                                                                                                 <  7:37505/149‑1 (MQ=39)
     aTCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGt                                                                                                                                             <  1:73550/149‑1 (MQ=39)
      tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCg                                                                                                                                              >  5:26718/1‑147 (MQ=39)
       cAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGtt                                                                                                                                            >  3:66566/1‑148 (MQ=39)
        aaCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTg                                                                                                                                           <  4:409028/148‑1 (MQ=39)
         aCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                    >  1:381722/1‑138 (MQ=40)
         aCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                    <  2:381722/138‑1 (MQ=40)
         aCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGaa                                                                                                                                         <  4:246641/149‑1 (MQ=39)
            aCTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGcc                                                                                                                                      >  5:249709/1‑149 (MQ=39)
             cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                     >  2:46489/1‑149 (MQ=39)
             cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                     <  6:119482/149‑1 (MQ=39)
                ggTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCgg                                                                                                                                    <  4:344868/147‑1 (MQ=39)
                ggTCGACACCACTACCGCGACCCTGACGGCGAGCCAGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAAACCTGCCCAGACGCCGCTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAACCCGGc                                                                                                                                   >  5:205083/1‑148 (MQ=255)
                    gACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCaaaa                                                                                                                               <  5:200665/148‑1 (MQ=39)
                    gACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGAc                                                                                                                              >  7:31054/1‑149 (MQ=25)
                    gACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAAc                                                                                                                              <  7:9841/149‑1 (MQ=40)
                     acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAACCCGGCAAGAcc                                                                                                                             >  3:154012/1‑149 (MQ=21)
                       accacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGAcc                                                                                                                             >  4:296016/1‑147 (MQ=17)
                       accacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCa                                                                                                                           <  5:66027/149‑1 (MQ=17)
                       accacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAAcc                                                                                                                             >  7:335070/1‑147 (MQ=38)
                        ccacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg                                                                                                                          <  5:407385/149‑1 (MQ=17)
                        ccacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAg                                                                                                                          <  4:66566/149‑1 (MQ=37)
                         cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTa                                                                                                                                                 <  6:199094/125‑1 (MQ=35)
                         cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGtt                                                                                                                                            >  6:345199/1‑130 (MQ=35)
                         cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg                                                                                                                          <  6:244686/148‑1 (MQ=17)
                         cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAgg                                                                                                                         >  1:64242/1‑149 (MQ=37)
                         cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAgg                                                                                                                         >  6:156450/1‑149 (MQ=37)
                          accaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCTACCCTGCCCAGACGCCGGGGACGGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAggg                                                                                                                        <  6:278574/146‑1 (MQ=11)
                          accaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAggg                                                                                                                        <  6:165857/146‑1 (MQ=0)
                          accaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAggg                                                                                                                        >  6:30595/4‑149 (MQ=0)
                          acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg                                                                                                                        <  1:46489/149‑1 (MQ=17)
                          acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg                                                                                                                        >  4:293433/1‑149 (MQ=17)
                          acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg                                                                                                                        >  3:326688/1‑149 (MQ=17)
                          acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAggg                                                                                                                        <  4:100658/149‑1 (MQ=37)
                           cTACCGCGACCCTGACTGCGCGCCCGTCGGTCACCGACGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCTAACCATTACCGTTGAAGCCGGCAAAACCCAGGGc                                                                                                                       <  8:89647/149‑1 (MQ=255)
                              ccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGc                                                                                                                    <  8:64336/149‑1 (MQ=12)
                               cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                     <  8:83998/131‑1 (MQ=32)
                               cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                     >  7:83998/1‑131 (MQ=32)
                                 cgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTc                                                                                                                 <  2:381736/149‑1 (MQ=34)
                                 cgACCCTGACGGCGAGCCCGTCGGTCACCCTAGGCGGCGTGATCACTTACTCCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTc                                                                                                                 <  2:115476/149‑1 (MQ=11)
                                   aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCGTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGa                                                                                                               <  3:143095/149‑1 (MQ=255)
                                         aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTc                                                                                                           <  2:29891/147‑1 (MQ=34)
                                           ggCGAGCCCGTCGGTCACCTAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCATTCCTGCCCAGACGCGGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACGGTTGAAGCCGTCAAAACCCAGGGCTGCGTCTATTTCCAGa                                                                                                       <  6:82933/149‑1 (MQ=11)
                                            gCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGAc                                                                                                      <  3:12338/149‑1 (MQ=34)
                                              gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                                                                                         <  6:83821/144‑1 (MQ=34)
                                              gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                                                                                         >  5:83821/1‑144 (MQ=34)
                                               aGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGAcccc                                                                                                   <  1:167581/149‑1 (MQ=255)
                                                gCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCg                                                                                                  <  5:325968/149‑1 (MQ=255)
                                                 cccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCgg                                                                                                 <  4:63464/149‑1 (MQ=255)
                                                       ggTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑t                                                                                           <  2:64242/148‑1 (MQ=34)
                                                       ggTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑Tg                                                                                          <  8:335070/149‑1 (MQ=34)
                                                         tCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGAc                                                                                        <  5:30595/149‑1 (MQ=255)
                                                            ccGAAGGCGGCGTGATCACTTACACCGTGACACCTTAGCAACCCTGCCCAGACGCCGGTGACCTTGACCCTGTCCAACGGCCAAACGATTACCTTTGAAGCCGGCAAAACCCAGTGCAGCGTCGACTTCCAGCCCCCGGCGAA‑TGAct                                                                                       >  1:257825/1‑147 (MQ=11)
                                                             cGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTc                                                                                     >  3:293575/1‑148 (MQ=34)
                                                             cGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTc                                                                                                                 >  3:340264/1‑121 (MQ=12)
                                                             cGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTc                                                                                                                 <  4:340264/121‑1 (MQ=12)
                                                             cGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGAc                                                                                        >  5:131463/1‑145 (MQ=255)
                                                              gAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTa                                                                                   >  3:190189/1‑149 (MQ=34)
                                                              gAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCTa                                                                                   <  1:317910/149‑1 (MQ=255)
                                                              gAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCTa                                                                                   <  3:399291/149‑1 (MQ=255)
                                                                 ggcggcGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTacaa                                                                                <  2:178396/149‑1 (MQ=34)
                                                                  gcggcgTGATCAATTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTacaac                                                                               <  4:293575/149‑1 (MQ=21)
                                                                    ggcgTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTCGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGt                                                                                      <  8:65911/140‑1 (MQ=21)
                                                                    ggcgTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGACCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTAcaacaa                                                                             <  8:17738/149‑1 (MQ=21)
                                                                        tGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGt                                                                         <  5:156450/149‑1 (MQ=34)
                                                                              cTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACt                                                                   <  2:45940/149‑1 (MQ=255)
                                                                              cTTACACCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACt                                                                   <  3:195409/149‑1 (MQ=255)
                                                                                  cacCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCa                                                               <  6:87509/149‑1 (MQ=255)
                                                                                   acCGTGAC‑CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGt                                                                 >  8:343492/1‑146 (MQ=255)
                                                                                    ccGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTGGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAgt                                                             <  6:26718/149‑1 (MQ=25)
                                                                                     ggcgctaccTGTGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAgt                                                             <  7:239971/142‑1 (MQ=255)
                                                                                       tGAC‑GCTGAGCAATGCTGCCGAGTCGCCGGTGACAGTGACCCTGTCCAACGTCCAAACCATTACCTTTGAAGGCGGCAAAACCCATGGCTGCTTCGATTTCCAGACCCCGGCGAA‑TGACGTCTTCAACAACGGTTCGACTGTCAGTGTc                                                          <  5:23564/149‑1 (MQ=11)
                                                                                          c‑ccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAG‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCAc                                                        >  7:270398/1‑148 (MQ=37)
                                                                                                aGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCg                                                   <  4:190189/148‑1 (MQ=37)
                                                                                                  cAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCa                                                               >  7:291436/1‑134 (MQ=34)
                                                                                                  cAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCa                                                               <  8:291436/134‑1 (MQ=34)
                                                                                                    aTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAAc                                              <  8:38966/149‑1 (MQ=37)
                                                                                                       cTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTGTCCATCGAGAACGc                                            >  1:354721/1‑148 (MQ=255)
                                                                                                          cccAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAACTGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCAc                                         <  1:104187/149‑1 (MQ=255)
                                                                                                             aGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAACCGGCAAAACCCAGGGCACCGTCGATTTCCACACCCCGGCGAA‑TGACGTCTACCACAACGGATCGACAGTCAGTGTCACCATCGACAACGCCACcggc                                     >  4:174936/1‑149 (MQ=11)
                                                                                                                    ggTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCa                                                               >  7:6541/1‑116 (MQ=35)
                                                                                                                    ggTGACAGTGAACCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCAGGCCAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCAACATCGAGAACGCCACCGGCGGTAAtt                              >  8:13156/1‑149 (MQ=255)
                                                                                                                        accgTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑t                                                                                           >  5:413015/4‑84 (MQ=1)
                                                                                                                        accgTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑t                                                                                           <  6:413015/81‑1 (MQ=1)
                                                                                                                          aGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCga                                                  <  4:382404/123‑1 (MQ=35)
                                                                                                                          aGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                         >  6:119983/1‑148 (MQ=38)
                                                                                                                           gTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCt                                                                                    <  1:17690/88‑1 (MQ=255)
                                                                                                                           gTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCt                                                                                    <  1:24444/88‑1 (MQ=255)
                                                                                                                           gTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑TGACGTCt                                                                                    >  2:17690/1‑88 (MQ=255)
                                                                                                                                   gTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGAccc               >  5:167883/1‑149 (MQ=37)
                                                                                                                                      cAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCg             >  8:195123/1‑148 (MQ=37)
                                                                                                                                      cAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATAGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCg             >  1:167190/1‑148 (MQ=37)
                                                                                                                                       aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGaa           >  7:179630/1‑149 (MQ=37)
                                                                                                                                       aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAA‑t                                                                                           <  1:329292/69‑1 (MQ=255)
                                                                                                                                             cAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGAc     >  3:322797/1‑149 (MQ=39)
                                                                                                                                                aCCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCCCCGGCGGGAATTTCGCGCCACTGACCCCGCATCCGACGcc  >  5:306072/1‑149 (MQ=14)
                                                                                                                                                aCCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGcc  >  5:29468/1‑149 (MQ=39)
                                                                                                                                                 ccATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGAccc               <  8:284909/135‑1 (MQ=37)
                                                                                                                                                  |                                                                                                                                                   
CCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAC‑CCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAA‑TGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCC  >  NC_002947/196206‑196497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑