Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,772,369 C→T R149R (CGG→CGA)  PP_2425 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,772,3690CT84.9% 119.4 / 13.3 53R149R (CGG→CGA) PP_2425AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base T (23/22);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATGCGGGCGAAGTGCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACGGTCGATGCCCACCGCCACGC  >  NC_002947/2772224‑2772514
                                                                                                                                                 |                                                                                                                                                 
cGATGCGGGCGAAGTGCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGt                                                                                                                                                <  6:297745/149‑1 (MQ=255)
         cGAAGTGCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACtt                                                                                                                                       >  5:103586/1‑149 (MQ=255)
            aGTGCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTcg                                                                                                                                    <  8:330297/149‑1 (MQ=255)
               gCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTcgcg                                                                                                                                  >  8:125775/1‑148 (MQ=255)
               gCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCACGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTcgcg                                                                                                                                  >  6:409972/1‑148 (MQ=255)
                ccccTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTc                                                                                                                                              >  3:95749/1‑135 (MQ=255)
                ccccTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTc                                                                                                                                              <  4:95749/135‑1 (MQ=255)
                ccccTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCa                                                                                                                                <  4:322600/149‑1 (MQ=255)
                  ccTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATg                                                                                                                              >  8:234178/1‑149 (MQ=255)
                  ccTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTAGTTCCACACTTTCGCGCATg                                                                                                                              >  1:183990/1‑149 (MQ=255)
                   cTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATgg                                                                                                                             >  4:131859/1‑149 (MQ=255)
                          tCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATc                                                                                                                      >  7:48942/1‑149 (MQ=255)
                                      cGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTc                                                                                                                                     <  1:412633/122‑1 (MQ=255)
                                      cGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTc                                                                                                                                     >  2:412633/1‑122 (MQ=255)
                                      cGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTgccgcc                                                                                                            >  5:336873/1‑147 (MQ=255)
                                      cGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGa                                                                                                          <  1:62785/149‑1 (MQ=255)
                                            acacCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATgg                                                                                                                             <  1:218560/124‑1 (MQ=255)
                                            acacCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATgg                                                                                                                             >  2:218560/1‑124 (MQ=255)
                                              acCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATc                                                                                                   >  3:230036/1‑148 (MQ=255)
                                                   ccTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGc                                                                                             <  3:131859/149‑1 (MQ=255)
                                                    cTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCg                                                                                            >  4:270001/1‑149 (MQ=255)
                                                    cTGCGGGCCACGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCg                                                                                            <  5:409972/149‑1 (MQ=255)
                                                       cGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCggg                                                                                          <  7:125775/148‑1 (MQ=255)
                                                            cgcgCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCcac                                                                                                                                           >  7:298012/1‑94 (MQ=255)
                                                            cgcgCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCcac                                                                                                                                           <  8:298012/94‑1 (MQ=255)
                                                                 aGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTg                                                                               <  8:112013/149‑1 (MQ=255)
                                                                   cgcgGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTg                                                                                                                          >  4:134435/1‑104 (MQ=255)
                                                                   cgcgGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTg                                                                                                                          <  3:134435/104‑1 (MQ=255)
                                                                                 ccTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGt                                                               >  2:147655/1‑149 (MQ=255)
                                                                                   tCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCa                                                                        >  4:128633/1‑138 (MQ=255)
                                                                                   tCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCa                                                                        <  3:128633/138‑1 (MQ=255)
                                                                                        tACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGc                                                        <  6:336873/149‑1 (MQ=255)
                                                                                             gcgcCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATg                                                   <  6:103586/149‑1 (MQ=255)
                                                                                                      ccAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCAc                                           >  4:357758/1‑148 (MQ=255)
                                                                                                      ccAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCAc                                           >  5:332226/1‑148 (MQ=255)
                                                                                                        aGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGt                                                               >  3:156408/1‑126 (MQ=255)
                                                                                                        aGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGt                                                               <  4:156408/126‑1 (MQ=255)
                                                                                                            ccTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCg                                    >  8:141321/1‑149 (MQ=255)
                                                                                                            ccTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCg                                    >  1:346839/1‑149 (MQ=255)
                                                                                                             cTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGc                                   <  7:234178/149‑1 (MQ=255)
                                                                                                              ttGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCg                                                                 <  4:374905/118‑1 (MQ=255)
                                                                                                              ttGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCg                                                                 >  3:374905/1‑118 (MQ=255)
                                                                                                                ggCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCCCTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGccc                                 <  1:298429/148‑1 (MQ=255)
                                                                                                                 gCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAg                               <  7:199846/149‑1 (MQ=255)
                                                                                                                      aaGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGc                          >  4:79550/1‑149 (MQ=255)
                                                                                                                      aaGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGc                          <  4:230036/149‑1 (MQ=255)
                                                                                                                       aGCGGGTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGcc                         >  2:283723/1‑149 (MQ=255)
                                                                                                                          gggTCCTGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACg                      <  3:270001/149‑1 (MQ=255)
                                                                                                                               cTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACGGTCGa                 >  4:409207/1‑149 (MQ=255)
                                                                                                                                tGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACgg                     <  8:353972/144‑1 (MQ=255)
                                                                                                                                tGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACgg                     >  7:353972/1‑144 (MQ=255)
                                                                                                                                tGCAGGCAGTGCAGCAGTCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACGGTCGAt                >  3:58117/1‑149 (MQ=255)
                                                                                                                                        gTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACGGTCGATGCCCACCg        >  4:15695/1‑149 (MQ=255)
                                                                                                                                              cagtCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACGGTCGATGCCCACCGCCAccc  >  2:406816/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGATGCGGGCGAAGTGCCCCTGTTGCTCCACCAGTGCCCGCAACACACCGGCCTGCGGGCCGCGCAGCGCGGTATACAGCACCTCGCGTACGCGCGCCGGGCCCAGTACCTTGGCGTCAAGCGGGTCCTGCAGGCAGTGCAGCAGCCGTTCCACACTTTCGCGCATGGGTGCATCCAGTGCCGCCGAGGTCATCGATTGCGGGGTTTGCGCCTGCACTTCAGCGTCCGGTGCCAAGCCCATGCTCTGCACCAACTCGCCCAGCACGCCACGGTCGATGCCCACCGCCACGC  >  NC_002947/2772224‑2772514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑