Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,948,139 G→A A112V (GCC→GTC)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,948,1390GA97.4% 246.0 / ‑2.9 79A112V (GCC→GTC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (32/44);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAG  >  NC_002947/5948004‑5948281
                                                                                                                                       |                                                                                                                                               
aaGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGT‑AACGCTc                                                                                                                                   <  2:192574/149‑1 (MQ=255)
aaGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTc                                                                                                                                   >  7:52621/1‑149 (MQ=255)
aaGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTc                                                                                                                                   <  6:422986/149‑1 (MQ=255)
 aGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAg                                                                                                                                            <  4:33475/140‑1 (MQ=255)
 aGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACATTCAGCAGGACGCAGT‑AACGCTcc                                                                                                                                  >  2:376464/1‑149 (MQ=255)
 aGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTAGCGGTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAg                                                                                                                                            >  3:33475/1‑140 (MQ=255)
  gTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTccc                                                                                                                                 <  8:21135/149‑1 (MQ=255)
     cTCGGTGGAGCCGTTACGGTCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTGCGGGCGGTCGAAGTTGATGGTATCGAGCTTCAGCAGGACGAAGT‑AACGCTCCcct                                                                                                                               <  7:240448/148‑1 (MQ=255)
     cTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCcctt                                                                                                                              <  3:159130/149‑1 (MQ=255)
      tCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCccttc                                                                                                                             >  5:125300/1‑149 (MQ=255)
      tCGGTGGAGCCGTTACCGGCCTCAATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCccttc                                                                                                                             >  2:205188/1‑149 (MQ=255)
        ggTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCccttc                                                                                                                             <  6:125300/147‑1 (MQ=255)
         gTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCta                                                                                                                                   >  2:322200/1‑139 (MQ=255)
         gTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCccttcctt                                                                                                                          <  1:13002/149‑1 (MQ=255)
          tGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCcttccttc                                                                                                                         <  1:50658/149‑1 (MQ=255)
            gAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCgg                                                                                                                       >  8:355275/1‑149 (MQ=255)
              gCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTg                                                                                                                     <  5:332361/149‑1 (MQ=255)
                cGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTggg                                                                                                                   >  4:228872/1‑149 (MQ=255)
                 gTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGc                                                                                                                  <  7:266272/149‑1 (MQ=255)
                 gTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGc                                                                                                                  <  8:52621/149‑1 (MQ=255)
                 gTTACAGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGAGTTTTCCGGGCGGTCGAAGTTGATGCTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGc                                                                                                                  >  2:110993/1‑149 (MQ=255)
                    aCCGGCCTCCATCTTCAGGCGCTTGTTCGGGATCAGCGGCGTCAGGTTTTCGAACATGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCgg                                                                                                                <  8:90866/148‑1 (MQ=255)
                        gCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCtt                                                                                                           >  2:345993/1‑149 (MQ=255)
                            ccATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCccttc                                                                                                                             >  5:32939/1‑127 (MQ=255)
                            ccATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCccttc                                                                                                                             <  6:32939/127‑1 (MQ=255)
                            ccATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGAAGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCgg                                                                                                                <  1:422272/140‑1 (MQ=255)
                            ccATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCACGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTACGGGCGGTCGAAGTTGAAGGTATCCACCTTCAGCAGGACGAACT‑AACCCTCCCCATACGTCGGTGGGCGGaagatccg                                                                                                        >  2:422272/1‑141 (MQ=255)
                             cATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGcc                                                                                                        <  5:47554/147‑1 (MQ=255)
                                   cAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGAt                                                                                                  <  1:166044/147‑1 (MQ=255)
                                    aGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGgt                                                                                               <  1:276934/149‑1 (MQ=255)
                                          ttGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGcc                                                                                         >  5:143047/1‑149 (MQ=255)
                                            gTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATTGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCg                                                                                        <  5:270067/148‑1 (MQ=255)
                                            gTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACACTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGcc                                                                                         <  5:150938/147‑1 (MQ=255)
                                             ttCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCcgac                                                                                                     <  1:208814/134‑1 (MQ=255)
                                               agggAAAATCGGCGTTACGTTTTTGAAAACGAATTTGTTTTTCGCGTTTTACCGGCGGTCGAATTTTATTGTATCGACCTTTAGCATGACCAATTCAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGt                                                                                      <  1:320916/147‑1 (MQ=255)
                                                       gcggcgTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTgg                                                                                                                    <  3:405068/109‑1 (MQ=255)
                                                       gcggcgTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTgg                                                                                                                    >  4:405068/1‑109 (MQ=255)
                                                         ggcgTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGa                                                                           >  7:126223/1‑148 (MQ=255)
                                                               aGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                     <  6:387202/148‑1 (MQ=255)
                                                               aGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                     <  6:143047/148‑1 (MQ=255)
                                                                       gAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggc                                                            <  4:84844/149‑1 (MQ=255)
                                                                       gAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctgg                                                             <  3:228872/148‑1 (MQ=255)
                                                                        aaCAGGATCTTGTTCTTCGCGTTTTCTGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCGCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggct                                                           <  7:381556/149‑1 (MQ=255)
                                                                        aaCAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggct                                                           >  1:67245/1‑149 (MQ=255)
                                                                        aaCAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggct                                                           <  4:8916/149‑1 (MQ=255)
                                                                            ggATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGc                                                       <  7:187919/149‑1 (MQ=255)
                                                                               tCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGc                                                       >  5:63420/1‑146 (MQ=255)
                                                                                       ttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGa                                             >  3:72994/1‑148 (MQ=255)
                                                                                         cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCTGATCTTGCCGACGATGGTGTCGCAGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGa                                             <  3:75830/146‑1 (MQ=255)
                                                                                         cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGatat                                          >  2:183179/1‑149 (MQ=255)
                                                                                          gcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTc                                                                                            <  3:109587/98‑1 (MQ=255)
                                                                                          gcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTc                                                                                            >  4:109587/1‑98 (MQ=255)
                                                                                           cgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCg                                        >  5:235799/1‑149 (MQ=255)
                                                                                           cgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCg                                        >  2:216317/1‑149 (MQ=255)
                                                                                                    ggCGGTCTTAGTTGATGGTATCGACCTGCAGCAGGACGATGT‑AACGCTCACCTTCCTTCGGTGGGCGGATCTTGCCGAGGATGGTGTCGCCGGTACGCAGGTTGACACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGggccg                               <  1:110993/149‑1 (MQ=255)
                                                                                                    ggCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGggccg                               <  8:126223/149‑1 (MQ=255)
                                                                                                       ggTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTTTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGggccggc                             >  5:329175/1‑148 (MQ=255)
                                                                                                       ggTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGat                                            >  6:324757/1‑133 (MQ=255)
                                                                                                       ggTCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGat                                            <  5:324757/133‑1 (MQ=255)
                                                                                                         tCGAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAg                          >  5:349861/1‑149 (MQ=255)
                                                                                                           gAAGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAgg                         >  6:301487/1‑148 (MQ=255)
                                                                                                            aaGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAgg                         >  5:237453/1‑147 (MQ=255)
                                                                                                             aGTTGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAg                      <  4:72994/149‑1 (MQ=255)
                                                                                                                tGATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGAc                   >  7:179716/1‑149 (MQ=255)
                                                                                                                 taTGGTATGGAGCTTCAGCATGTCGAAGT‑AACGCTCCCCTTCCTTCGGTGGTCTGATCTTGCGGACGATGGTGTCGCCTGTACGCAGTTTGAAAGGTCGGATCTGGCTGGGCGTGACATTGATATCGTCTGGGCCGGCCAGGTAGGAc                   <  8:273130/147‑1 (MQ=255)
                                                                                                                 gATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGAc                   <  6:349861/148‑1 (MQ=255)
                                                                                                                 gATGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGAc                   <  1:183179/148‑1 (MQ=255)
                                                                                                                  aTGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGc                 <  6:235799/149‑1 (MQ=255)
                                                                                                                   tGGTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGAACTGGCTGGGCGAGACATAGATATCGACTGGGCCGGCCAGGTAGGAc                   >  7:273130/1‑146 (MQ=255)
                                                                                                                     gTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAGCGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCAt               <  1:205188/148‑1 (MQ=255)
                                                                                                                     gTATCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATc              <  1:216317/149‑1 (MQ=255)
                                                                                                                       aTCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCgaga                                                   <  5:246429/110‑1 (MQ=255)
                                                                                                                       aTCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCgaga                                                   >  6:246429/1‑110 (MQ=255)
                                                                                                                       aTCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTGCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTAGGCAGGTTGAACCGGCGGATCTGGCTGGGCGAGACAGAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAg            >  6:89504/1‑149 (MQ=255)
                                                                                                                        tCGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGc           >  7:99015/1‑149 (MQ=255)
                                                                                                                         cGACCTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGc           <  6:63420/148‑1 (MQ=255)
                                                                                                                             cTTCAGCAGGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAAc      <  8:99015/149‑1 (MQ=255)
                                                                                                                                 agcagGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCa   <  2:67245/148‑1 (MQ=255)
                                                                                                                                 agcagGACGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAg  <  8:214281/149‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                               
AAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGT‑AACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAG  >  NC_002947/5948004‑5948281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑