| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 98.0% | 147.4 / NA | 50 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (16/33); total (16/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 98.0% | 150.3 / NA | 50 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (15/34); total (16/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAT > NC_002947/1499339‑1499607 || cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGCAACCGGCGCCGCCGTGCATGGCACCGGcgt > 8:5123/1‑147 (MQ=255) aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 5:402378/149‑1 (MQ=255) gggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcc > 6:177790/1‑149 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 5:177790/149‑1 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 7:130337/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 4:190120/149‑1 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtgt < 3:115057/149‑1 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtgt > 8:386743/1‑148 (MQ=255) gACAACGCTGAAGCCGGGGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTCAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg < 2:344956/148‑6 (MQ=37) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg > 1:179962/1‑138 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 2:179962/138‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcggg < 8:282482/149‑7 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcggg < 2:334515/149‑7 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg < 5:210617/148‑6 (MQ=255) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCCCGCAACACGCGCCGCCGGGCATGGCAACCGCGTTTGCGGTGttagcgggc > 1:244194/1‑142 (MQ=255) cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑cg‑‑‑c < 6:143415/149‑1 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGCTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 1:86309/131‑1 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg > 2:86309/1‑130 (MQ=255) cggTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGGGGGCGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCca < 5:3116/147‑1 (MQ=21) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCcac < 5:123918/149‑1 (MQ=25) ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGACCGCGAAACAGGCGCCGCCGTGCATGGCACCGGCGTTGCCGGTGTTAGCGGGCA‑CGC‑‑CCGCTCCCACACACTGAt > 3:347383/1‑149 (MQ=255) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCC‑TGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCAtt > 5:179756/1‑148 (MQ=25) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCgg < 8:199500/105‑1 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCgg > 7:199500/1‑105 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGc < 7:5123/149‑1 (MQ=21) cTGAACGACGCCTTGCCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGGGGGCG‑GGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACGGGCTTTGCGGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGa < 1:369487/131‑1 (MQ=18) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCgg < 7:350340/149‑1 (MQ=21) ttatttaaGCTTTGGCACCACAGCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGTTCGCGGGCA‑GGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGc < 8:169742/140‑1 (MQ=18) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg > 2:420352/1‑101 (MQ=255) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 1:420352/101‑1 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGGGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAACCGGGCCTAt < 3:337228/149‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACCGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 1:315619/149‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 1:63180/149‑1 (MQ=21) gcggCCAAACGGGGGGCGCGGGGG‑GGCCCGCGAAACTGGCGCCGCGGGGCAGGCCCCCGGCTTTGCGGGGGTG‑CGGGGGC‑CGCCCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 1:334998/146‑1 (MQ=255) gCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCA‑CGC‑‑CCGCTCCCACAGCCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg > 5:412635/1‑149 (MQ=21) ccAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc < 8:230629/120‑1 (MQ=17) ccAAACGGGGGGAGCGGGCG‑TGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 2:244194/147‑1 (MQ=21) aCGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa < 1:330003/149‑1 (MQ=21) aCGGTGGGAGCCGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt > 3:302048/1‑148 (MQ=21) gCGGGCG‑TGCCCGCTAAACCGGCGCCGCGGTCAATGGCACGGGCTTTGCCGGTGTTCGCGGGAA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 3:33342/149‑1 (MQ=25) gggCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 6:6373/1‑149 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 7:131089/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 3:133517/136‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt > 4:133517/1‑136 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:164368/1‑146 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGATACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 1:109485/149‑1 (MQ=37) aCAGGCCCCGCGGGGCATGGCACCGGCTTTGCCGGTGTTCGCGGCCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 6:241116/149‑1 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 6:179756/149‑1 (MQ=37) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 6:95521/1‑149 (MQ=37) aGGCGACTCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt < 7:19295/149‑1 (MQ=37) || CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCG‑TGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAT > NC_002947/1499339‑1499607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |