Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,218,668 Δ1 bp 15.4% intergenic (+51/+32) oprJ → / ← nfxB outer membrane protein OprJ/HTH‑type transcriptional regulator NfxB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,218,6680G.15.4% 113.7 / 15.2 39intergenic (+51/+32)oprJ/nfxBouter membrane protein OprJ/HTH‑type transcriptional regulator NfxB
Reads supporting (aligned to +/- strand):  ref base G (16/17);  new base . (3/3);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

AAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGCTC  >  NC_002947/3218526‑3218814
                                                                                                                                               |                                                                                                                                                  
aaGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTgg                                                                                                                                              <  5:94421/149‑1 (MQ=255)
 agcagcCTTCATCGAAGCCACTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTATGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTggg                                                                                                                                             >  5:290699/1‑149 (MQ=255)
           aTCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTgg                                                                                                                                   <  4:176294/149‑1 (MQ=255)
            tCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGc                                                                                                                                  >  8:76650/1‑149 (MQ=255)
              gAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCt                                                                                                                                 <  8:198053/148‑1 (MQ=255)
                   cAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTg                                                                                                                             >  3:263065/1‑147 (MQ=255)
                    aGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAg                                                                                                                          >  2:204128/1‑149 (MQ=255)
                      tACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGc                                                                                                                        <  1:204128/149‑1 (MQ=255)
                      caCCCCGCGACCACTGGCGGAGGTCCACCTGTTCCGCGCCCCCGGCGGCGGCGGGCCGAGCACGGGCTGAAACCGCCTCCTTTGGTTGGCTTGGCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGc                                                                                                                        <  4:263065/148‑1 (MQ=255)
                        cccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAg                                                                                                                      <  2:246237/149‑1 (MQ=255)
                         ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAgg                                                                                                                     >  3:158632/1‑149 (MQ=255)
                         ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAgg                                                                                                                     >  3:346470/1‑149 (MQ=255)
                            gCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCa                                                                                                                  <  7:240799/149‑1 (MQ=255)
                                 aaCTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg                                                                                                                                             <  3:148974/117‑1 (MQ=255)
                                 aaCTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg                                                                                                                                             >  4:148974/1‑117 (MQ=255)
                                    tGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCgg                                                                                                          >  8:36317/1‑149 (MQ=255)
                                    tGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCgg                                                                                                          >  2:161445/1‑149 (MQ=255)
                                              cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg                                                                                                                                             <  3:174975/104‑1 (MQ=255)
                                              cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg                                                                                                                                             >  6:174983/1‑104 (MQ=255)
                                              cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg                                                                                                                                             <  5:174983/104‑1 (MQ=255)
                                              cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg                                                                                                                                             >  4:174975/1‑104 (MQ=255)
                                                aCCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCAc                                                                                              <  7:76650/149‑1 (MQ=255)
                                                  cTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCg                                                                                            <  6:290699/149‑1 (MQ=255)
                                                             aCTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcagc                                                                                                >  4:100055/1‑134 (MQ=255)
                                                             aCTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcagc                                                                                                <  3:100055/134‑1 (MQ=255)
                                                                       ggcTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGc                                                                       <  1:161445/149‑1 (MQ=255)
                                                                            gCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGttt                                                                   <  7:36317/148‑1 (MQ=255)
                                                                                     gggCTGAAACAGCCTCCTTTGGAAGGATTGTCAAGCCACTTGGCCCAAGGG‑ATCGATGCAATGGGGTTTTTTTGGCTg                                                                                                                                >  7:221568/1‑78 (MQ=255)
                                                                                                      tttGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGtt                                                                    >  7:294676/1‑121 (MQ=255)
                                                                                                      tttGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGtt                                                                    <  8:294676/121‑1 (MQ=255)
                                                                                                             ggCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGcgtc                                  <  4:158632/148‑1 (MQ=255)
                                                                                                                    ccAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCa                          >  3:341069/1‑149 (MQ=255)
                                                                                                                     cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCg                             <  4:328233/145‑1 (MQ=255)
                                                                                                                     cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCg                             >  3:328233/1‑145 (MQ=255)
                                                                                                                     cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCAt                         >  5:315250/1‑149 (MQ=255)
                                                                                                                     cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCAt                         >  4:236534/1‑149 (MQ=255)
                                                                                                                        ccACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGTAGGCAGGTCAGCGAGCGGGTGCCgcagc                                                                                                <  1:194112/75‑1 (MQ=255)
                                                                                                                        ccACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcagc                                                                                                >  2:194112/1‑75 (MQ=255)
                                                                                                                                             aTGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGCTc  <  4:341069/149‑1 (MQ=255)
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AAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCATCGACGATGCCGTACACCATGCTC  >  NC_002947/3218526‑3218814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑