| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 107,990 | Δ1 bp | 7.0% | coding (451/642 nt) | PP_5426 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 107,990 | 0 | C | . | 7.0% | 182.8 / 7.0 | 57 | coding (451/642 nt) | PP_5426 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (27/26); new base . (2/2); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAGGCAGTCCAG > NC_002947/107842‑108131 | cACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTc < 5:175919/149‑1 (MQ=255)cACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGAGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTc < 5:170962/149‑1 (MQ=255) ccAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGTTGATTGGCGAAAGCTTT‑GCCaa < 5:326596/149‑1 (MQ=255) ccACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACtg < 1:106362/147‑1 (MQ=255) ccACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGATTGGCGAAAGCTTT‑GCCAACCa < 6:332733/143‑1 (MQ=255) ccACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGATTGGCGAAAGCTTT‑GCCAACCa > 5:332733/1‑143 (MQ=255) cACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACtggtg < 6:154482/149‑1 (MQ=255) cgccgcGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTggg < 2:181454/149‑1 (MQ=255) gCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCaa > 6:44554/1‑115 (MQ=255) gCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCaa < 5:44554/115‑1 (MQ=255) tctcGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCg < 8:12382/136‑1 (MQ=255) tctcGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCg > 7:12382/1‑136 (MQ=255) tcGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGa > 7:204334/1‑149 (MQ=255) gATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCAACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGAt > 1:186979/1‑148 (MQ=255) tCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGgctg > 7:242261/1‑149 (MQ=255) cACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGgctgct > 8:135586/1‑149 (MQ=255) ccGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAATGGctgctg > 2:61216/1‑148 (MQ=255) ccGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGgctgcttg < 8:204334/149‑3 (MQ=255) ccGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGAGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTATCGCCAATCAACTGGTGGGCAATCAGGTTGGAGACCGTGGTGTCGACCGGTATCAATGGatgctg > 2:276870/1‑148 (MQ=255) aTTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt > 3:161325/1‑109 (MQ=255) aTTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt < 4:161325/109‑1 (MQ=255) ttCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTTGTGTCGACCGGGATCAATGGCTGCTGGAAGt < 1:276870/149‑1 (MQ=255) ttCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAATGGCTGCTGGAAGt < 1:61216/149‑1 (MQ=255) tCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTg > 7:74436/1‑149 (MQ=255) cAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTgg > 7:131590/1‑149 (MQ=255) aaCGGCAAAGCTCTCATCATTAAGCATTTCGTCAACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGc < 2:186979/149‑1 (MQ=255) ctcATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGt > 3:208667/1‑149 (MQ=255) tcatcaTTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGt > 6:41808/1‑148 (MQ=255) tcatcaTTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGt > 7:206717/1‑148 (MQ=255) catcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCa > 4:105552/1‑149 (MQ=255) atcatTAAGCATTTGGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt > 7:299600/1‑90 (MQ=255) atcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt < 8:299600/90‑1 (MQ=255) atcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAt > 7:26892/1‑149 (MQ=255) tcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAtt < 8:26892/149‑1 (MQ=255) tcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAtt < 1:241743/149‑1 (MQ=255) tcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGAGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAt > 3:306841/1‑148 (MQ=255) ttAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCgtg < 2:302898/96‑1 (MQ=255) ttAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCgtg > 1:302898/1‑96 (MQ=255) aGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACct < 8:242261/148‑1 (MQ=255) cgtcgACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTccac > 2:345269/1‑148 (MQ=255) gtcgACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGCCCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTccac > 1:250985/1‑147 (MQ=255) tggtgCACCACCTCATCCAGCTGGTTGAC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTAc > 8:164693/1‑117 (MQ=255) tggtgCACCACCTCATCCAGCTGGTTGAC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTAc < 7:164693/117‑1 (MQ=255) gtgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGAt < 3:325668/111‑1 (MQ=255) gtgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGAt > 4:325668/1‑111 (MQ=255) tgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAg < 8:131590/149‑1 (MQ=255) tgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAg < 8:206717/149‑1 (MQ=255) accTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGc < 7:135586/149‑1 (MQ=255) tCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAgg < 4:237101/141‑1 (MQ=255) tCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAgg > 3:237101/1‑141 (MQ=255) ccaccAGTTGATTGGCGAAAGCTTT‑GCCAACCAGCTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCa > 3:166125/4‑149 (MQ=38) aTCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGc < 7:140559/95‑1 (MQ=255) aTCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGc > 8:140559/1‑95 (MQ=255) aTCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGAGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCa < 4:306841/148‑1 (MQ=255) cAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATcc < 2:250985/148‑1 (MQ=255) aGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATc > 3:281479/1‑146 (MQ=255) gTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAg < 4:281479/147‑1 (MQ=255) aaGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAGGCAGTCCAg < 2:7392/149‑1 (MQ=255) aaGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACAGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTAATACAGAAGGCAGTCCAg > 5:287303/1‑149 (MQ=255) | CACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAGGCAGTCCAG > NC_002947/107842‑108131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |