| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,751,089 | Δ1 bp | 12.5% | coding (448/855 nt) | PP_1551 → | replication protein O |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,751,089 | 0 | G | . | 12.5% | 84.9 / 8.2 | 32 | coding (448/855 nt) | PP_1551 | replication protein O |
| Reads supporting (aligned to +/- strand): ref base G (11/17); new base . (2/2); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-02 | |||||||||||
TGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAGTCCAAGG > NC_002947/1750942‑1751236 | tGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCAGCCGCCCTTCGTTAGTCTAATAAAGCAACCCCCTATGTAACATCTTCCGAAGGAAGATg > 6:320224/1‑149 (MQ=255) aTTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTAc < 4:182556/149‑1 (MQ=255) tttACGTTGATCCGAAACAGACCAAAACAGCCTACTCGGATGAAGTGGTCCGAATCGTCTCATAGTCGAAACAGAACAAAACCGACGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTAc < 5:272977/148‑1 (MQ=255) tCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACGGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAACATCTTCCGAAGGAAGAT‑TTACAtagg < 7:128556/143‑4 (MQ=255) tCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACGGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAACATCTTCCGAAGGAAGAT‑TTACAtagg > 8:128556/1‑140 (MQ=255) ccGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGACCGACCCGATGTAA‑ATCTTCATTCGGAAGATGTTACATTCCCCCCCAg > 2:91944/1‑149 (MQ=255) cGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGc < 7:184008/149‑1 (MQ=255) cGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGc < 1:344990/149‑1 (MQ=255) gAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCGAAGGAAGAT‑TTACa < 4:226012/135‑1 (MQ=255) gAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCGAAGGAAGAT‑TTACa > 3:226012/1‑135 (MQ=255) gACTCGGCTGAAGTGGTCCGACTCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAGCCCCCTATGTTA‑ATCTTACTTAGGAAGATGTTACTTTAAAGAACAGCGAGCCGGA‑GCCCACTcctg > 2:131595/1‑146 (MQ=255) gACTCGGCTGAAGTGGTCCGACTCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAGCCCCCTATGTAA‑ATCTTACTTCGGAAGATGTTAGATTCAACCCCAGCCAACCGGA‑GCCGCCACCGGc > 2:126460/1‑149 (MQ=255) cTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGAt < 7:194398/149‑1 (MQ=255) gAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAGGCCCCTATGTAA‑ATCTTCCGTCGGAAGATGGTAGATGCCGCCGCAGCGAGCCGGC‑GCCGACTCCGCCCAAGGATGa > 8:151995/1‑149 (MQ=255) tttATTCTAAGAAAGAGACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGACCACCCAGAGCGCCGGA‑GCCCCCTCCGCCCACCGAAGAGCGCAAGGCGCCCGACGGCCAAGCCGCC‑ATGCTGGCCGACAAACCGCACgcggag > 4:215786/1‑144 (MQ=255) tttATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GTCGGCTCCGGctc < 1:62285/87‑3 (MQ=255) aTTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGa < 7:151995/148‑1 (MQ=255) ttCTAAGAAAGAAACCCCCTATGTAA‑TTCTTCCTTCGGAAGATGTTACATGCCCACCCTGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCATGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGAt < 1:277351/148‑1 (MQ=255) aagaaagaAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCt < 3:313387/140‑1 (MQ=255) aagaaagaAACCCCCTATGTAA‑ATCTTACTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑AGCCTGGCCCACAAACCCCACCCGCTCGAGGCATc > 4:125890/1‑149 (MQ=255) agaaagaaACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAAt < 3:174565/147‑1 (MQ=255) cttAA‑ATCTTCCTTCGGAATATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTAc < 5:19740/146‑1 (MQ=255) aa‑aTCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTACGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTat > 6:19740/1‑144 (MQ=255) aa‑aTCTTCCTTCGGAAGATGTTAAATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCTTTGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACtt < 1:91944/148‑1 (MQ=255) a‑aTCTTCCTTCGGAATATGTTACATGCCCCCCCAGCGATCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg < 1:131595/148‑1 (MQ=255) a‑aTCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg < 3:125890/148‑1 (MQ=255) a‑aTCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg < 1:126460/148‑1 (MQ=255) ttccttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTc > 7:63448/1‑149 (MQ=255) ttccttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTc > 8:268978/1‑149 (MQ=255) cttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGc < 3:215786/149‑1 (MQ=255) ttcGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGc > 6:37099/1‑148 (MQ=255) gAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAGTc > 7:137862/1‑149 (MQ=255) tGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAGTCCAAg < 5:40301/148‑1 (MQ=255) gTTACATGCCCCCCCAGCGAGCCGGAGGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAGTCCAAgg > 1:181193/1‑149 (MQ=255) | TGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCGGAAGATGTTACATGCCCCCCCAGCGAGCCGGA‑GCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCC‑ATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAAGTCCAAGG > NC_002947/1750942‑1751236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |