Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947307,8651.C98.4% 205.0 / ‑3.7 61intergenic (+880/‑989)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (32/28);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307718‑308008
                                                                                                                                                     |                                                                                                                                               
aaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCCGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGTCAGCCGAAA‑gg                                                                                                                                                 <  5:313665/149‑2 (MQ=255)
   ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgt                                                                                                                                             >  3:196443/1‑146 (MQ=255)
       gTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑g                                                                                                                                                  >  1:71321/1‑141 (MQ=255)
       gTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑g                                                                                                                                                  <  2:71321/141‑1 (MQ=255)
             ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATc                                                                                                                                     <  8:241991/148‑1 (MQ=255)
             ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCg                                                                                                                                    <  5:318343/149‑1 (MQ=255)
                  aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgac                                                                                                                               >  1:72344/1‑149 (MQ=255)
                    aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgac                                                                                                                               <  8:545/147‑1 (MQ=255)
                    aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgac                                                                                                                               >  7:545/1‑147 (MQ=255)
                    aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgtta                                                                                                                                           >  1:169666/1‑129 (MQ=255)
                    aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgtta                                                                                                                                           <  2:169666/134‑6 (MQ=255)
                      cTTCCTGCTGCCGCCTGCCGATGTACTGCCTATGCACAGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTATTCGGCCTGACCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCCGTgacgac                                                                                                                            >  8:178818/1‑148 (MQ=255)
                           tgctgcCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCAc                                                                                                                      >  1:97392/1‑149 (MQ=255)
                            gctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACg                                                                                                                     >  6:25802/1‑149 (MQ=255)
                                ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                                 <  6:262869/149‑1 (MQ=255)
                                        cgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTTCCCTTTCGGCTTACGAAA‑GCCGTTACCTGATGGGTGACGACGAGCACGGTTGGGGCGaa                                                                                                          <  7:178818/148‑1 (MQ=255)
                                             tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                    <  2:72344/149‑1 (MQ=255)
                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                                  >  5:45150/1‑149 (MQ=255)
                                                  ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                               <  6:301260/149‑1 (MQ=255)
                                                  ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                               <  5:25802/149‑1 (MQ=255)
                                                   cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCTGCCTGTACGGCACTTGCAAGACTACCCTTTCGGCTGACGAAA‑GCCTTTACATGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTTTCtt                                                                                               >  1:168069/1‑148 (MQ=255)
                                                     aTGTACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                                 >  6:144637/1‑144 (MQ=255)
                                                     aTGTACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                                 <  5:144637/144‑1 (MQ=255)
                                                      tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                            <  6:45150/148‑1 (MQ=255)
                                                       gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa                                                                                          >  5:48165/1‑149 (MQ=255)
                                                         aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGAt                                                                                                                                      <  6:115243/103‑1 (MQ=255)
                                                              gcctccAACATCCTCGAAGTAGGCGATGTGACCTTGTTCTTCTGCCTGTCCGTCACTGGCAATACTACCCTTTCGGCTGACGAAATA‑CGTTACCTGATAGGTGAAGACTAGCACGGTTGGGGCGAAGGAGTTGTATTCAACATGTAAgg                                                                                   <  2:168069/145‑1 (MQ=255)
                                                                ggCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcg                                                                                 >  6:342996/1‑149 (MQ=255)
                                                                  ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAAc                                                                                           <  8:94942/137‑1 (MQ=255)
                                                                  ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAAc                                                                                           >  7:94942/1‑137 (MQ=255)
                                                                  ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                               <  2:97392/149‑1 (MQ=255)
                                                                   cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                              >  1:48469/1‑149 (MQ=255)
                                                                    aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcg                                                                                 >  5:210347/1‑145 (MQ=255)
                                                                    aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcg                                                                                 <  6:210347/145‑1 (MQ=255)
                                                                       aTCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa                                                                          >  5:205620/1‑149 (MQ=255)
                                                                           gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       >  1:301495/1‑148 (MQ=255)
                                                                           gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       >  5:51813/1‑148 (MQ=255)
                                                                                            aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                     >  1:88119/1‑149 (MQ=255)
                                                                                            aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                     <  2:48469/149‑1 (MQ=255)
                                                                                                   tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGAACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                                 >  6:360139/1‑146 (MQ=255)
                                                                                                     ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            >  7:90882/1‑149 (MQ=255)
                                                                                                     ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            >  2:143354/1‑149 (MQ=255)
                                                                                                              tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                     <  5:342996/147‑1 (MQ=255)
                                                                                                                cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTc                                  <  6:205620/148‑1 (MQ=255)
                                                                                                                  gCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATc                               <  2:88119/149‑1 (MQ=255)
                                                                                                                    aCTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCATCATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                             <  2:301495/149‑1 (MQ=255)
                                                                                                                          aaGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt                                                      <  1:40156/118‑1 (MQ=255)
                                                                                                                          aaGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt                                                      >  2:40156/1‑118 (MQ=255)
                                                                                                                            gACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAg                        >  8:284530/1‑146 (MQ=255)
                                                                                                                            gACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAg                        <  7:284530/146‑1 (MQ=255)
                                                                                                                              cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                    >  3:209585/1‑148 (MQ=255)
                                                                                                                              cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                    >  7:41573/1‑148 (MQ=255)
                                                                                                                              cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                    <  5:360139/148‑1 (MQ=255)
                                                                                                                              cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   <  6:48165/149‑1 (MQ=255)
                                                                                                                                 cccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                >  3:310144/1‑149 (MQ=255)
                                                                                                                                  ccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            >  5:256844/1‑120 (MQ=255)
                                                                                                                                  ccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                            <  6:256844/120‑1 (MQ=255)
                                                                                                                                  ccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                >  2:177995/1‑148 (MQ=255)
                                                                                                                                  ccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                >  8:281426/1‑148 (MQ=255)
                                                                                                                                  ccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                >  3:260375/1‑148 (MQ=255)
                                                                                                                                          cTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCa       <  4:260375/149‑1 (MQ=255)
                                                                                                                                           tGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa                 >  4:230351/1‑138 (MQ=255)
                                                                                                                                           tGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa                 <  3:230351/138‑1 (MQ=255)
                                                                                                                                           tGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg      >  2:159534/1‑149 (MQ=255)
                                                                                                                                             aCGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTa    <  7:281426/149‑1 (MQ=255)
                                                                                                                                             aCGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTa    <  1:177995/149‑1 (MQ=255)
                                                                                                                                               gAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  >  5:323403/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307718‑308008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑