| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,218,661:1 | +C | 15.8% | intergenic (+44/+39) | oprJ → / ← nfxB | outer membrane protein OprJ/HTH‑type transcriptional regulator NfxB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,218,661 | 1 | . | C | 15.8% | 109.3 / 22.1 | 38 | intergenic (+44/+39) | oprJ/nfxB | outer membrane protein OprJ/HTH‑type transcriptional regulator NfxB |
| Reads supporting (aligned to +/- strand): ref base . (16/16); new base C (3/3); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
GCTACGTGAACGAAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCAT > NC_002947/3218514‑3218791 | gCTACGTGAACGAAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑a < 4:121490/149‑1 (MQ=255) aaGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTgg < 5:94421/149‑1 (MQ=255) agcagcCTTCATCGAAGCCACTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTATGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTggg > 5:290699/1‑149 (MQ=255) aTCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTgg < 4:176294/149‑1 (MQ=255) tCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGc > 8:76650/1‑149 (MQ=255) gAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCt < 8:198053/148‑1 (MQ=255) cAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTg > 3:263065/1‑147 (MQ=255) aGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAg > 2:204128/1‑149 (MQ=255) tACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGc < 1:204128/149‑1 (MQ=255) caCCCCGCGACCACTGGCGGAGGTCCACCTGTTCCGCGCCCCCGGCGGCGGCGGGCCGAGCACGGGCTGAAACCGCCTCCTTTGGTTGGCTTGGCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGc < 4:263065/148‑1 (MQ=255) cccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAg < 2:246237/149‑1 (MQ=255) ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAgg > 3:158632/1‑149 (MQ=255) ccAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAgg > 3:346470/1‑149 (MQ=255) gCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCa < 7:240799/149‑1 (MQ=255) aaCTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg < 3:148974/117‑1 (MQ=255) aaCTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg > 4:148974/1‑117 (MQ=255) tGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCgg > 8:36317/1‑149 (MQ=255) tGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCgg > 2:161445/1‑149 (MQ=255) cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg > 6:174983/1‑104 (MQ=255) cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg < 5:174983/104‑1 (MQ=255) cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg > 4:174975/1‑104 (MQ=255) cAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGGCATCGAT‑CCCTggg < 3:174975/104‑1 (MQ=255) aCCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCAc < 7:76650/149‑1 (MQ=255) cTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCg < 6:290699/149‑1 (MQ=255) aCTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcagc < 3:100055/134‑1 (MQ=255) aCTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcagc > 4:100055/1‑134 (MQ=255) ggcTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGc < 1:161445/149‑1 (MQ=255) gCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGttt < 7:36317/148‑1 (MQ=255) gggCTGAAACAGCCTCCTTTGGAAGGATTGTCAAGCCACTTGGCCCAAGGG‑ATCGATGCAATGGGGTTTTTTTGGCTg > 7:221568/1‑78 (MQ=255) tttGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGtt > 7:294676/1‑121 (MQ=255) tttGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGtt < 8:294676/121‑1 (MQ=255) ggCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGcgtc < 4:158632/148‑1 (MQ=255) ccAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCa > 3:341069/1‑149 (MQ=255) cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCg > 3:328233/1‑145 (MQ=255) cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCg < 4:328233/145‑1 (MQ=255) cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCAt > 4:236534/1‑149 (MQ=255) cAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCAt > 5:315250/1‑149 (MQ=255) ccACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGTAGGCAGGTCAGCGAGCGGGTGCCgcagc < 1:194112/75‑1 (MQ=255) ccACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCgcagc > 2:194112/1‑75 (MQ=255) | GCTACGTGAACGAAGCAGCCTTCATCGAAGCCAGTACCCAGCGACAACTGGCGTTGGTCAACCTGTTCCGCGCACTCGGCGGCGGCTGGCCGAGCAAGGGCTGAAACAGCCTCCTTTGGTTGGCTTGTCCAGCCACTTGGCCCCAGGG‑ATCGATGCCCTGGGGTTTTTTTGGCTGGTGAAGGCAGGTCAGCGAGCGGGTGCCGCAGCACCGTCGAGGAAAAGCAGCTCCAGCGTTTGCACGGACGTCGCGCTGGCCGCCCGCCCGCGTCGTTCGGCAT > NC_002947/3218514‑3218791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |