Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 336,124:1 +T 100% coding (209/237 nt) PP_0278 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947336,1241.T97.6% 130.9 / ‑3.1 41coding (209/237 nt)PP_0278hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (17/23);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCC  >  NC_002947/335980‑336249
                                                                                                                                                  |                                                                                                                             
aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑ttac                                                                                                                           <  2:133869/149‑4 (MQ=255)
 cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑ttacc                                                                                                                          >  8:324465/1‑145 (MQ=255)
     tCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtgtg                                                                                                                       <  1:117879/149‑1 (MQ=255)
       ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGc                                                                                                                      <  3:242458/148‑1 (MQ=255)
        cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtgt                                                                                                                        >  8:138263/1‑145 (MQ=255)
        cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtgt                                                                                                                        <  7:138263/145‑1 (MQ=255)
            aTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATg                                                                                                                <  7:324465/149‑1 (MQ=255)
                      cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGgcgc                                                                                                      <  4:128393/149‑1 (MQ=255)
                              ccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                              <  4:263906/149‑1 (MQ=255)
                                       ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGTGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAAAGATATc                                                                                        >  5:154077/1‑146 (MQ=255)
                                       ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                        >  6:169327/1‑146 (MQ=255)
                                        cGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                        >  2:61724/1‑145 (MQ=255)
                                                  gCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                  >  7:243735/1‑125 (MQ=255)
                                                  gCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                  <  8:243735/125‑1 (MQ=255)
                                                   ccTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                         <  6:154077/149‑1 (MQ=255)
                                                       cGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                     <  5:169327/149‑1 (MQ=255)
                                                              gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                <  1:209175/147‑1 (MQ=255)
                                                              gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                >  2:209175/1‑147 (MQ=255)
                                                               tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                             >  6:357658/1‑149 (MQ=255)
                                                               tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                             <  5:357658/149‑1 (MQ=255)
                                                                 tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTa                                                              <  4:90048/146‑1 (MQ=255)
                                                                 tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTa                                                              >  3:90048/1‑146 (MQ=255)
                                                                   gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                           <  1:61724/147‑1 (MQ=255)
                                                                   gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                         >  3:268525/1‑149 (MQ=255)
                                                                         gATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAAATCCTGGGGGCAGCCGAA‑TACCTGTGCGACAAGGCAAAGACGCTGATGAACCATATCGACATCGCCAAGCGTAAGGCGCATTACGCATTGAcgc                                                   >  6:40007/1‑149 (MQ=255)
                                                                                   aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                         >  5:49428/1‑149 (MQ=255)
                                                                                   aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                         >  3:246264/1‑149 (MQ=255)
                                                                                    tCAAGCACCTTGTGCGCGAGTGCTATATGTCGGACGAGCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCTCATTAGGCATTGACGCGGTCATTtgtg                                        <  5:40007/149‑1 (MQ=255)
                                                                                      aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTggg                                      >  8:164472/1‑149 (MQ=255)
                                                                                            cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc                                >  5:137283/1‑149 (MQ=255)
                                                                                                       gggCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                         <  6:296896/129‑1 (MQ=255)
                                                                                                       gggCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                         >  5:296896/1‑129 (MQ=255)
                                                                                                            atatGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATgg                <  7:164472/149‑1 (MQ=255)
                                                                                                                 gatgacgacCATAAATTCCTGGGGGGAGGGG‑ATTTCCTGTGCGACATGTCCAAGGCGCTGATGAACGATATCGAGATGGCCAAGGGTAAGGCGCATTAGTCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGTCTGc           <  6:49428/146‑1 (MQ=255)
                                                                                                                  aggaCGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCa          <  4:268525/149‑1 (MQ=255)
                                                                                                                     acgacCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag       <  1:268411/149‑1 (MQ=255)
                                                                                                                       gacCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag       >  4:248259/1‑147 (MQ=255)
                                                                                                                        acCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcag    <  6:137283/149‑1 (MQ=255)
                                                                                                                          cATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcc  <  4:246264/149‑1 (MQ=255)
                                                                                                                            tAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                             >  1:46232/1‑88 (MQ=255)
                                                                                                                            tAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                             <  2:46232/88‑1 (MQ=255)
                                                                                                                             aaaTTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCt             <  7:7066/135‑1 (MQ=255)
                                                                                                                             aaaTTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCt             >  8:7066/1‑135 (MQ=255)
                                                                                                                                                  |                                                                                                                             
ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCC  >  NC_002947/335980‑336249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑