| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,845,703:1 | +G | 100% | intergenic (‑70/+28) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 94.3% | 168.6 / 3.2 | 53 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base G (32/18); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845564‑3845842 | tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATc > 6:277481/1‑149 (MQ=255) catcatCAGTTGCTGGCTCTGCAAGCCTTCGCGCAGGCCGACTTCATTGATTGTTTTTGTTGGCATCGCGGTCTCTCTGTCTGAATCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATc < 4:7722/149‑1 (MQ=255) cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATc < 4:103630/149‑1 (MQ=255) aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCa < 5:277481/149‑1 (MQ=255) aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGc > 7:75544/1‑148 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCg < 5:116447/141‑1 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCg > 6:116447/1‑141 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGa > 7:140160/1‑149 (MQ=255) gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGAGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGa > 1:14272/1‑149 (MQ=255) cttgcagaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCCGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt < 2:170049/142‑1 (MQ=255) ctctGCTAGGCATCGCGCTGGACGAGTTGATTGATTGTTATTGTTGTCATCGCGGTCTCTGTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGG‑AGTAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCAggatggat < 4:184500/149‑1 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGAt < 8:188374/144‑1 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGAt > 7:188374/1‑144 (MQ=255) gcagaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCa < 6:92604/144‑1 (MQ=255) gcgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGATGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTg > 1:192728/1‑149 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGc > 1:337526/1‑149 (MQ=255) ggCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGAACCATAGTAAGCCACGATGGATCATCCCGCGCCCTGCCAgg > 1:131034/1‑149 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt > 6:135083/1‑127 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt < 5:135083/127‑1 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCg > 6:170779/1‑149 (MQ=255) tcttttattttttttgttGTCATCGCGGTCTCTCTGTCTTAAGCTTTG‑CGCCAGAATAAGTGCTGTTTCAGGGGA‑ATAAAACACGGTTTTCGGG‑GGCTTATCCATCTTCAGCCACTATGTATCAGCCCGCGCCCTGCCAGGCCGGTGc < 8:75544/142‑1 (MQ=255) attgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg < 5:274155/149‑1 (MQ=255) ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCAca > 3:198727/1‑147 (MQ=255) ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg > 3:204645/1‑148 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCCCgcag > 2:209272/1‑148 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTCTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCCGCCCGCGCCCTGCCAGGCCGGTGCACgcag > 6:284247/1‑148 (MQ=255) gttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCAAACTGCGCCACGATGGCTCAGCCCGCGCCCAGCCAGGCCGGTGGACGCAGCaa > 6:44534/1‑148 (MQ=255) gttttCATGGGGGTCTCTGTGTCTTAAG‑GTTGCCCCCATAATAAGAGCTGTAT‑CCGGGCAACAAAACCCGTGTTGC‑GCGGGCTCACCCATCGCCCCCCACTTGGTATCCGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg < 8:140160/148‑1 (MQ=255) tctctGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccga > 3:115434/1‑149 (MQ=255) tctctGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc > 5:301251/1‑147 (MQ=255) tgtgtctgAAGCGTTG‑CGCCAGACTATGAGCTGGATGACGGGC‑AGAAACGCCGTGTTTCGGG‑GGCTTATCCTTCGTCAGCCACGATGGATCTGCCCGCGCCCTGCCAGGCCGGTGc < 7:8731/114‑1 (MQ=255) tctgtctgAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGc > 8:8731/1‑116 (MQ=255) tctgtctgAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc > 1:103443/1‑149 (MQ=255) tctgtctgAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATACATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc > 1:163777/1‑138 (MQ=255) gAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCAGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc > 1:47454/1‑149 (MQ=255) aaGCGTTG‑CGCCTTATTAATAGCTGTTTCTGGGGC‑AAAAAAGACGTGTTTCGGG‑GGCTTATCCATCGTCAGCCAGGATGTATAAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa < 2:192728/146‑1 (MQ=255) aGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCga < 5:170779/149‑1 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt > 2:112469/1‑149 (MQ=255) caaaCTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc > 3:16156/4‑149 (MQ=255) aaGACTAAGAGCTGGATGACGGGA‑AGAAAACACGGGTTTC‑GCGGGCGGATCCATCGTCAGCCACGATGTTTCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc < 4:204645/148‑1 (MQ=255) gACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCg > 5:155126/1‑149 (MQ=255) gCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATACATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCa > 7:301794/1‑149 (MQ=255) tGGATGACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCAGATACATAGGGAGCCACGAAGGATAAGCCCCCCCCCAGGCAGGCCCGGGGAAGCCGCAAAAGGGGGTGAAACAGGCGCCGAGCCGCCACCGAGAGGGGgtggcggtggcgaga > 2:310563/1‑145 (MQ=255) gttGACGGGC‑AGAAAAGACGTGTGTC‑GCGGGCTGAGCCATCGTCATCCACGATGGAGCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCa < 1:209272/147‑1 (MQ=255) gACGGGC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGa < 4:115434/148‑1 (MQ=255) gC‑AGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCACGCGCCCTGGCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtggg > 7:160357/1‑124 (MQ=255) aGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt > 7:39558/1‑149 (MQ=255) gAAAAGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt < 6:155126/149‑1 (MQ=255) aaaaGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt > 6:299530/1‑148 (MQ=255) aGACGTGTTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGcc > 8:1423/1‑121 (MQ=255) gtgtTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGa < 1:214750/138‑1 (MQ=255) gtgtTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGa > 2:214750/1‑138 (MQ=255) gtgtTTC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc < 2:103443/149‑1 (MQ=255) | TCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTG‑CGCCAGACTAAGAGCTGGATGACGGGC‑AGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845564‑3845842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |