Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,666,304:1	+A	6.3%	coding (70/1854 nt)	nuoL →	NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,666,304	1	.	A	6.3%	251.3 / 11.4	63	coding (70/1854 nt)	nuoL	NADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base . (33/26);  new base A (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

ATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGT  >  NC_002947/4666158‑4666449                                                                                                                                                     |                                                                                                                                                  aTTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATAGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑gg                                                                                                                                                 >  8:299975/1‑149 (MQ=255)    ccgatctccATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTtc                                                                                                                                              <  3:273618/142‑1 (MQ=255)            cATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTATCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTtc                                                                                                                                              >  4:273618/1‑141 (MQ=255)                    tgcAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCg                                                                                                                              >  1:47067/1‑149 (MQ=255)                    tgcAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCg                                                                                                                              >  8:211973/1‑149 (MQ=255)                           gTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGACAGGTTCTCCGAGAAc                                                                                                                                     <  6:142716/136‑1 (MQ=255)                           gTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGACAGGTTCTCCGAGAAc                                                                                                                                     >  5:142716/1‑136 (MQ=255)                                 ccgcTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAAc                                                                                                                                     >  3:298129/1‑129 (MQ=255)                                 ccgcTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAAc                                                                                                                                     <  4:298129/129‑1 (MQ=255)                                  cgcTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTGCGCGGAGAACCTGTCCGCCCTGATCGGCGTc                                                                                                                >  6:286807/1‑149 (MQ=255)                                        cacaCTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCg                                                                                                          >  3:214876/1‑149 (MQ=255)                                         acacTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCgg                                                                                                         >  4:329088/1‑149 (MQ=255)                                            ctctCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAg                                                                                                      <  6:45587/149‑1 (MQ=255)                                                 gACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTGCTGCTGCTGTCGATTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAgg                                                                                                     <  1:100091/146‑1 (MQ=255)                                                 gACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGc                                                                                                    <  2:47067/146‑1 (MQ=255)                                                  aCATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGt                                                                                                        >  7:29732/1‑141 (MQ=255)                                                  aCATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGt                                                                                                        <  8:29732/141‑1 (MQ=255)                                                    aTCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTa                                                                                                       <  3:236641/140‑1 (MQ=255)                                                    aTCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTa                                                                                                       >  4:236641/1‑140 (MQ=255)                                                    aTCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCg                                                                                              <  7:211973/149‑1 (MQ=255)                                                              ccAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACgg                                                                                    >  4:252594/1‑149 (MQ=255)                                                                aGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGc                                                                                                    <  2:250079/131‑1 (MQ=255)                                                                aGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGc                                                                                                    >  1:250079/1‑131 (MQ=255)                                                                  tGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGgccgc                                                                                <  3:329088/149‑1 (MQ=255)                                                                          gcgGATGAACCTTCTCTTCCTGTCTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCa                                                                        >  2:245448/1‑149 (MQ=255)                                                                            ggATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATAGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGACCGCCTACGTCATc                                                                      <  7:299975/149‑1 (MQ=255)                                                                                                  tttCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGc                                                >  7:52748/1‑149 (MQ=255)                                                                                                     cGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTc                                                       >  3:80476/1‑139 (MQ=255)                                                                                                     cGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTc                                                       <  4:80476/139‑1 (MQ=255)                                                                                                        cTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAgg                                          >  8:134979/1‑149 (MQ=255)                                                                                                             cccTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGggcggc                                     >  7:49629/1‑149 (MQ=255)                                                                                                             cccTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGggcggc                                     >  2:108027/1‑149 (MQ=255)                                                                                                              ccTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAggg                                         <  8:161680/144‑1 (MQ=255)                                                                                                              ccTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAggg                                         >  7:161680/1‑144 (MQ=255)                                                                                                              ccTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGgcggcg                                    >  8:311825/1‑149 (MQ=255)                                                                                                                tCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGgcgc                                   >  5:188288/1‑148 (MQ=255)                                                                                                                tCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGcgcg                                  >  5:161577/1‑149 (MQ=255)                                                                                                                tCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGcgcg                                  >  5:207761/1‑149 (MQ=255)                                                                                                                  aTCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGgcgc                                   <  6:188288/146‑1 (MQ=255)                                                                                                                  aTCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGcgcg                                  <  4:214876/147‑1 (MQ=255)                                                                                                                     ggCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTAca                              <  8:49629/148‑1 (MQ=255)                                                                                                                           ctgctgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCCACGTCGCCCCGCCTGAGGGCGGCGCCTACAGCCAgct                       >  2:53057/1‑149 (MQ=255)                                                                                                                            tgctgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAgctg                      >  8:294988/1‑149 (MQ=255)                                                                                                                            tgctgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAgctg                      >  1:10503/1‑149 (MQ=255)                                                                                                                               tgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGc                                                                                                    >  1:292220/1‑68 (MQ=255)                                                                                                                               tgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGc                                                                                                    <  2:292220/68‑1 (MQ=255)                                                                                                                               tgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGgcgc                                   >  6:161283/1‑133 (MQ=255)                                                                                                                               tgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGgcgc                                   <  5:161283/133‑1 (MQ=255)                                                                                                                               tgctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGctgctg                   >  1:157264/1‑149 (MQ=255)                                                                                                                                 ctgTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCtgt                  <  8:52748/148‑1 (MQ=255)                                                                                                                                  tggcG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGGAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTgg                <  5:286807/146‑1 (MQ=255)                                                                                                                                    tCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTgg                >  1:7305/1‑147 (MQ=255)                                                                                                                                        ttCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCTCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTAcagcc                           <  4:20451/133‑1 (MQ=255)                                                                                                                                        ttCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTg           >  4:27235/1‑149 (MQ=255)                                                                                                                                        ttCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTg           <  7:311825/149‑1 (MQ=255)                                                                                                                                          ctcGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTgg          <  6:161577/148‑1 (MQ=255)                                                                                                                                           tcGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGAt        >  2:278792/1‑149 (MQ=255)                                                                                                                                           tcGCGCGGAC‑GGGTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTCCGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGAt        >  5:170028/1‑149 (MQ=255)                                                                                                                                           cagggcgGACAGGTTCTCCGAGAACC‑GTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCtgt                  <  3:293245/135‑1 (MQ=255)                                                                                                                                           cagggcgGACAGGTTCTCCGAGAACC‑GTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCtgt                  >  4:293245/5‑139 (MQ=255)                                                                                                                                             gcgcgGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGt      >  2:329930/1‑149 (MQ=255)                                                                                                                                             gcgcgGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGt      <  3:252594/149‑1 (MQ=255)                                                                                                                                               gcgGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCg    <  1:108027/149‑1 (MQ=255)                                                                                                                                                 ggAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGt  <  6:207761/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTTTCCCCTCATCGGCTTCCTGCTGCTGTCG‑TTCTCGCGCGGAC‑GGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGT  >  NC_002947/4666158‑4666449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑