Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,842,904:1	+G	25.6%	intergenic (+48/‑249)	ccoP‑II → / → PP_4259	cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,842,904	1	.	G	25.6%	74.1 / 40.9	39	intergenic (+48/‑249)	ccoP‑II/PP_4259	cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (14/15);  new base G (6/4);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

CCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCA  >  NC_002947/4842758‑4843052                                                                                                                                                    |                                                                                                                                                     ccGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑Tg                                                                                                                                                    <  7:141987/149‑1 (MQ=255)      ccAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGa                                                                                                                                               <  5:242810/149‑1 (MQ=255)          gAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCgg                                                                                                                                           <  1:211448/149‑1 (MQ=255)            acaacTCCAGGGCAACGACAATGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGTCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCAT‑TTTAGGAGCGGGt                                                                                                                                         <  6:95401/149‑1 (MQ=39)                cTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTAc                                                                                                                                     <  1:43894/149‑1 (MQ=255)                    aGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAg                                                                                                                                                 >  4:220250/1‑133 (MQ=255)                    aGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAg                                                                                                                                                 <  3:220250/133‑1 (MQ=255)                       gCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTTAGCCGGCCAAGGCTTATTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGTAGCGGGTTTACCCGCGaa                                                                                                                              <  8:98215/149‑1 (MQ=255)                       gCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTTAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGaa                                                                                                                              <  2:255249/149‑1 (MQ=255)                         aaCGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAaga                                                                                                                            <  2:298318/149‑1 (MQ=255)                         aaCGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCg                                                                                                                                            <  6:299669/133‑1 (MQ=255)                         aaCGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCg                                                                                                                                            >  5:299669/1‑133 (MQ=255)                              cAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGttt                                                                                                                                       <  2:27602/133‑1 (MQ=255)                              cAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGttt                                                                                                                                       >  1:27602/1‑133 (MQ=255)                                                 gcTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGT‑AACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTtctc                                                                                                   >  4:104714/1‑149 (MQ=255)                                                          tACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCAACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCg                                                                                                                       >  3:308046/1‑121 (MQ=255)                                                               ccTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTTAACCCCCGGCGACACAGGATGCG‑TGTAGGGGCGGGGTTTACCGCGGAGAGGCCGGGTCTGGCCCCGCATTTGTGGGAGGGAAGGGAc                                                                                      >  5:224285/1‑149 (MQ=255)                                                                            gCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGa                                                                         >  5:292456/1‑149 (MQ=255)                                                                                                gTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGttt                                                      >  5:152564/1‑148 (MQ=255)                                                                                                   aggcaggcCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATa                                                  <  3:72908/149‑1 (MQ=255)                                                                                                    ggcaggcCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATAc                                                 <  6:292456/149‑1 (MQ=255)                                                                                                                      gcgGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGAc                                >  8:209760/1‑148 (MQ=255)                                                                                                                             aaCCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGt                         <  7:209760/148‑1 (MQ=255)                                                                                                                                ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCATTTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAgg                          >  2:262954/1‑144 (MQ=255)                                                                                                                                ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCATTTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAgg                          <  1:262954/144‑1 (MQ=255)                                                                                                                                ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACCGGTTTCATACAACGAACCCAACGGGAGGAAAGGTCg                       >  4:274209/1‑147 (MQ=37)                                                                                                                                  gCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATaa                                                             >  8:217543/1‑107 (MQ=255)                                                                                                                                    tCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGc                 >  2:21381/1‑149 (MQ=255)                                                                                                                                      cTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGc                 <  6:152564/147‑1 (MQ=255)                                                                                                                                      cTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCg                >  6:46098/1‑148 (MQ=255)                                                                                                                                       tACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGaa              >  1:261184/1‑149 (MQ=255)                                                                                                                                        acacAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAc             >  6:333106/1‑149 (MQ=255)                                                                                                                                         acaaGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAc             >  7:163580/4‑149 (MQ=255)                                                                                                                                          acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAc             <  1:190505/147‑1 (MQ=255)                                                                                                                                          acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAc             >  2:190505/1‑147 (MQ=255)                                                                                                                                           aaGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCg                                                                   <  6:257005/92‑1 (MQ=255)                                                                                                                                           aaGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCg                                                                   >  5:257005/2‑93 (MQ=255)                                                                                                                                              gATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTCGATCAAATGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATAc       >  5:278479/1‑149 (MQ=255)                                                                                                                                                  cT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGcc   <  3:104714/149‑1 (MQ=255)                                                                                                                                                   t‑tGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCa  >  6:338763/1‑149 (MQ=255)                                                                                                                                                   t‑tGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCa  >  2:44000/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCA  >  NC_002947/4842758‑4843052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑