Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,059,508:1	+G	11.9%	coding (1326/1581 nt)	PP_4458 ←	opine ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,059,508	1	.	G	11.9%	206.5 / 25.6	59	coding (1326/1581 nt)	PP_4458	opine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (25/27);  new base G (2/5);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01

TGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGTG  >  NC_002947/5059362‑5059648                                                                                                                                                    |                                                                                                                                             tGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑Ga                                                                                                                                            >  5:34384/1‑149 (MQ=255)   tttCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑Ga                                                                                                                                            <  7:279494/147‑1 (MQ=255)   tttCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACa                                                                                                                                          >  3:87758/1‑149 (MQ=255)        gACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGct                                                                                                                                     <  5:138912/149‑2 (MQ=255)        gACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGct                                                                                                                                     <  3:321043/149‑2 (MQ=255)              acaaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGctcagtag                                                                                                                               <  2:32114/149‑8 (MQ=255)                aaaGGGATGGCGCATATGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCGCAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGAAGCGGGt                                                                                                                               >  5:261563/1‑147 (MQ=255)                aaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATAAGCCTCGACCCTGGATTTCCGGGTGGCATCATCAGATACTGAGCAATGTGCGAATTTG‑GACATTGCAGCGGGTgc                                                                                                                             >  2:327681/1‑149 (MQ=255)                  aGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTgcgc                                                                                                                           <  4:87758/149‑1 (MQ=255)                   gggATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCgg                                                                                                                                 >  1:276962/1‑142 (MQ=255)                   gggATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCgg                                                                                                                                 <  2:276962/142‑1 (MQ=255)                     taTGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGa                                                                                                                        <  7:302584/148‑1 (MQ=255)                       tGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATg                                                                                                                      <  1:28360/149‑1 (MQ=255)                          cgcATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCa                                                                                                                   <  7:251710/149‑1 (MQ=255)                                  tcgtcgtGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCg                                                                                                            >  7:54443/1‑148 (MQ=255)                                        tGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTa                                                                                                     <  8:54443/149‑1 (MQ=255)                                                     gcgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGa                                                                                                                        <  5:344361/117‑1 (MQ=255)                                                     gcgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGa                                                                                                                        >  6:344361/1‑117 (MQ=255)                                                            tttCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCagtag                                                                                  <  6:34384/148‑1 (MQ=255)                                                                   aaaTCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGcc                                                                          >  6:89450/1‑149 (MQ=255)                                                                         aGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGata                                                                    <  5:203039/149‑1 (MQ=255)                                                                          gTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               >  6:346287/1‑137 (MQ=255)                                                                          gTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               <  5:346287/137‑1 (MQ=255)                                                                               gTCTGCGGATCAGACTCGACCCTGGCTTTACGGATGTCATCATCAGCTACTGAGCAATGTCAGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAATCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCTCCCGGATATCGatc                                                              <  1:327681/149‑3 (MQ=255)                                                                                          aGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               >  6:361083/1‑121 (MQ=255)                                                                                          aGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               <  5:361083/121‑1 (MQ=255)                                                                                           gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTaaaa                                                  >  1:320404/1‑149 (MQ=255)                                                                                           gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTaaaa                                                  >  5:70637/1‑149 (MQ=255)                                                                                            ccTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGACGCCGAATCGTAAATCTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTaaaa                                                  >  3:296095/1‑148 (MQ=255)                                                                                            ccTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAac                                                 <  5:89450/149‑1 (MQ=255)                                                                                             cTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               <  6:166667/118‑1 (MQ=255)                                                                                             cTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAaca                                                >  6:70021/1‑149 (MQ=255)                                                                                              tCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAacac                                               >  7:281478/1‑149 (MQ=255)                                                                                                    cTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATcgc                                         <  8:281478/149‑1 (MQ=255)                                                                                                        cTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGctc                                     >  7:105046/1‑149 (MQ=255)                                                                                                           tCCGGATGGAATCATCAGATACTTACCAATGTCCTAAATTC‑GACATTGCAGCGGGTGCGCCTATGGCATAAGCCGAATCGTAAAACTCTGTCAGCCATTAGTCGGCGCCCGTATATCGAGCAGCACCGTAAAACACGATCGCGCTCTg                                   <  6:70637/148‑1 (MQ=255)                                                                                                            ccGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCTGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATc                                                                  <  3:299727/116‑1 (MQ=255)                                                                                                            ccGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCTGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATc                                                                  >  4:299727/1‑116 (MQ=255)                                                                                                                 tGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTa                                                     <  7:292692/124‑1 (MQ=255)                                                                                                                 tGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTa                                                     >  8:292692/1‑124 (MQ=255)                                                                                                                    catcatCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTc                                <  7:275595/142‑1 (MQ=255)                                                                                                                    aatcatcAGCTACTGAGCAATGTCAGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGACGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCTAGCAGAACCGTAAAACACGATCGCGCTCTggac                                >  8:275595/2‑140 (MQ=255)                                                                                                                     atcatcAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGc                        >  7:10052/1‑149 (MQ=255)                                                                                                                                cTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg              <  8:105046/148‑1 (MQ=255)                                                                                                                                  gAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTa           >  5:220070/1‑149 (MQ=255)                                                                                                                                   tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAAt                                                                                                         >  3:128650/2‑54 (MQ=18)                                                                                                                                   tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAAt                                                                                                         <  4:128650/53‑1 (MQ=18)                                                                                                                                   tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTg                   >  6:196205/2‑140 (MQ=255)                                                                                                                                   tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTg                   <  5:196205/139‑1 (MQ=255)                                                                                                                                   aGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTaa          <  6:220070/149‑1 (MQ=255)                                                                                                                                    gCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                               <  6:339342/95‑1 (MQ=255)                                                                                                                                    gCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                               >  5:339342/1‑95 (MQ=255)                                                                                                                                      aaTGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTaa          >  6:16545/1‑146 (MQ=255)                                                                                                                                      aaTGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTaa          <  5:16545/146‑1 (MQ=255)                                                                                                                                            cGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGTg  <  3:169499/148‑1 (MQ=255)                                                                                                                                             gAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg              >  1:271029/1‑135 (MQ=255)                                                                                                                                             gAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg              <  2:271029/135‑1 (MQ=255)                                                                                                                                                 ttC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGt            >  3:268626/1‑133 (MQ=255)                                                                                                                                                 ttC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGt            <  4:268626/133‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             TGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGTG  >  NC_002947/5059362‑5059648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑