| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,385,226:1 | +G | 7.1% | intergenic (+19/‑44) | lldP → / → lldD | L‑lactate permease/L‑lactate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,385,226 | 1 | . | G | 7.1% | 219.2 / 11.4 | 56 | intergenic (+19/‑44) | lldP/lldD | L‑lactate permease/L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (30/22); new base G (2/2); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01 | |||||||||||
GTCGGCAAGGAATCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCTG > NC_002947/5385085‑5385374 | gTCGGCAAGGAATCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTcgcg > 5:36099/1‑149 (MQ=255)gTCGGCAAGGAATCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTcgc < 3:199429/148‑1 (MQ=255) gCAAGGAATCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGcc > 2:84108/1‑149 (MQ=255) gAATCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCGCGACG‑GCGCCCGGcc > 4:305552/1‑148 (MQ=255) tCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCGCGACG‑GCGCCCGGcccct < 3:305552/149‑5 (MQ=255) gACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTcgc < 7:118451/133‑1 (MQ=255) gACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTcgc > 8:118451/1‑133 (MQ=255) aCCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCAACCTGGTCCAGGCTTACTGGCTGACCGGCTTGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCca > 5:120510/1‑148 (MQ=255) cTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCcac > 4:282780/1‑147 (MQ=255) cTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACaa < 3:282780/149‑1 (MQ=255) cGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACg > 2:236477/1‑149 (MQ=255) ttCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAg > 6:6809/1‑141 (MQ=255) ttCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAg < 5:6809/141‑1 (MQ=255) cACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGc < 1:84108/148‑1 (MQ=255) ccTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCCCAAGCAACGCTGCGAGAAGCCATGaggat > 7:230902/1‑145 (MQ=255) ttcGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGcc > 6:168405/1‑149 (MQ=255) cGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCTTGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGc < 6:120510/146‑1 (MQ=255) caccaTCGTCGGCCTGATCACCCTGGTCCTGGATTACTGGCTGACCGTCATGCTGGTTCATCACTAAAGTGAATGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTAc < 7:295457/149‑1 (MQ=255) caccaTCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGcc > 1:283402/1‑97 (MQ=255) caccaTCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGcc < 2:283402/97‑1 (MQ=255) accaTCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTAcc > 6:112621/1‑149 (MQ=255) gCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATcgcg > 8:52426/1‑149 (MQ=255) gATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATc < 3:32720/142‑1 (MQ=255) gATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATc > 4:32720/1‑142 (MQ=255) aTCCCCCTGGTCCAGGCTTACTGGCTTACCGGCATGCTGGTTCATCAATAAAGTCAACGGGGCCGGGCGC‑GGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGcgccgc < 8:282753/148‑1 (MQ=255) cccTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGccc < 5:168405/149‑1 (MQ=255) tGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTAcc > 2:293323/1‑127 (MQ=255) tGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAAc > 7:315103/1‑149 (MQ=255) cAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCaa < 1:236477/148‑1 (MQ=255) cAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAg > 3:16332/1‑149 (MQ=255) aGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCca < 1:43211/79‑1 (MQ=255) aGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCca > 2:43211/1‑79 (MQ=255) ggCTTACTGGCTGATCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCt > 5:75803/1‑149 (MQ=255) gCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTg > 4:103024/1‑149 (MQ=255) cTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTg > 7:101757/1‑144 (MQ=255) cTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTg < 8:101757/144‑1 (MQ=255) gCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTACCACAAGCAACGCTGCGAGAATACAAGATAATTTATGCCTCTAACGAATATCCCCCCCCGGCCCAACGCaa > 2:315354/1‑137 (MQ=255) cGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGc > 1:321093/1‑133 (MQ=255) cGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGc < 2:321093/133‑1 (MQ=255) cGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGtt > 8:8314/1‑149 (MQ=255) ggCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTc < 7:8314/149‑1 (MQ=255) ggCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTc < 3:103024/149‑1 (MQ=255) atcaCTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAAc < 6:30337/113‑1 (MQ=255) atcaCTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAAc > 5:30337/1‑113 (MQ=255) tAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCCAGCTGCCCCCCTTCCTGTTCCCATACGCTGACggcggc > 8:261810/1‑149 (MQ=255) tAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACggcggc > 2:315100/1‑149 (MQ=255) aaGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGgcggcg < 4:16332/148‑1 (MQ=255) tGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTa > 4:302247/1‑149 (MQ=255) gAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTAc > 6:3506/1‑149 (MQ=255) aaaGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACg > 2:302259/1‑149 (MQ=255) aGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGcc > 5:142246/1‑149 (MQ=255) gggCCGGGCGC‑CGTCGCGCTCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGa < 8:315103/149‑1 (MQ=255) tgCCGGGCGCGCGACG‑GCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCAttt < 6:343774/62‑1 (MQ=25) tgCCGGGCGCGCGACG‑GCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCAttt > 5:343774/2‑63 (MQ=25) ggCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAg < 6:142246/149‑1 (MQ=255) gggCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGcaca < 1:315100/149‑1 (MQ=255) gcgc‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACAcc < 6:75803/149‑1 (MQ=255) gcgc‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACAcc > 6:34583/1‑149 (MQ=255) c‑cGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCTg > 1:4003/1‑149 (MQ=255) | GTCGGCAAGGAATCCGACCTGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGC‑CGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCTG > NC_002947/5385085‑5385374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |