Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 107,981:1 +G 6.9% coding (460/642 nt) PP_5426 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947107,9811.G 6.9% 227.4 / 11.3 58coding (460/642 nt)PP_5426hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (27/27);  new base G (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CAGGCACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAG  >  NC_002947/107838‑108122
                                                                                                                                                |                                                                                                                                             
cAGGCACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGc                                                                                                                                          >  1:181454/1‑149 (MQ=255)
    cACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTc                                                                                                                                      <  5:175919/149‑1 (MQ=255)
    cACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGAGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTc                                                                                                                                      <  5:170962/149‑1 (MQ=255)
         ccAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGTTGATTGGCGAAAGCTTT‑GCCaa                                                                                                                                 <  5:326596/149‑1 (MQ=255)
                  ccACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACtg                                                                                                                          <  1:106362/147‑1 (MQ=255)
                  ccACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGATTGGCGAAAGCTTT‑GCCAACCa                                                                                                                              >  5:332733/1‑143 (MQ=255)
                  ccACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGATTGGCGAAAGCTTT‑GCCAACCa                                                                                                                              <  6:332733/143‑1 (MQ=255)
                   cACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACtggtg                                                                                                                       <  6:154482/149‑1 (MQ=255)
                     cgccgcGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTggg                                                                                                                     <  2:181454/149‑1 (MQ=255)
                                               gCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCaa                                                                                                                             <  5:44554/115‑1 (MQ=255)
                                               gCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCaa                                                                                                                             >  6:44554/1‑115 (MQ=255)
                                                    tctcGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCg                                                                                                   <  8:12382/136‑1 (MQ=255)
                                                    tctcGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCg                                                                                                   >  7:12382/1‑136 (MQ=255)
                                                      tcGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGa                                                                                    >  7:204334/1‑149 (MQ=255)
                                                        gATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCAACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGAt                                                                                   >  1:186979/1‑148 (MQ=255)
                                                                tCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGgctg                                                                          >  7:242261/1‑149 (MQ=255)
                                                                  cACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGgctgct                                                                        >  8:135586/1‑149 (MQ=255)
                                                                    ccGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAATGGctgctg                                                                       >  2:61216/1‑148 (MQ=255)
                                                                    ccGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGgctgcttg                                                                      <  8:204334/149‑3 (MQ=255)
                                                                    ccGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGAGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTATCGCCAATCAACTGGTGGGCAATCAGGTTGGAGACCGTGGTGTCGACCGGTATCAATGGatgctg                                                                       >  2:276870/1‑148 (MQ=255)
                                                                       aTTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt                                                                                                           <  4:161325/109‑1 (MQ=255)
                                                                       aTTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt                                                                                                           >  3:161325/1‑109 (MQ=255)
                                                                        ttCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTTGTGTCGACCGGGATCAATGGCTGCTGGAAGt                                                                  <  1:276870/149‑1 (MQ=255)
                                                                        ttCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAATGGCTGCTGGAAGt                                                                  <  1:61216/149‑1 (MQ=255)
                                                                         tCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTg                                                                 >  7:74436/1‑149 (MQ=255)
                                                                          cAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTgg                                                                >  7:131590/1‑149 (MQ=255)
                                                                           aaCGGCAAAGCTCTCATCATTAAGCATTTCGTCAACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGc                                                               <  2:186979/149‑1 (MQ=255)
                                                                                       ctcATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGt                                                   >  3:208667/1‑149 (MQ=255)
                                                                                        tcatcaTTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGt                                                   >  6:41808/1‑148 (MQ=255)
                                                                                        tcatcaTTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGt                                                   >  7:206717/1‑148 (MQ=255)
                                                                                         catcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCa                                                 >  4:105552/1‑149 (MQ=255)
                                                                                          atcatTAAGCATTTGGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt                                                                                                           >  7:299600/1‑90 (MQ=255)
                                                                                          atcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGtt                                                                                                           <  8:299600/90‑1 (MQ=255)
                                                                                          atcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAt                                                >  7:26892/1‑149 (MQ=255)
                                                                                           tcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAtt                                               <  1:241743/149‑1 (MQ=255)
                                                                                           tcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAtt                                               <  8:26892/149‑1 (MQ=255)
                                                                                           tcatTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGAGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCAt                                                >  3:306841/1‑148 (MQ=255)
                                                                                              ttAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCgtg                                                                                                 >  1:302898/1‑96 (MQ=255)
                                                                                              ttAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCgtg                                                                                                 <  2:302898/96‑1 (MQ=255)
                                                                                                 aGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACct                                          <  8:242261/148‑1 (MQ=255)
                                                                                                        cgtcgACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTccac                                   >  2:345269/1‑148 (MQ=255)
                                                                                                         gtcgACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGCCCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTccac                                   >  1:250985/1‑147 (MQ=255)
                                                                                                                   tggtgCACCACCTCATCCAGCTGGTTGAC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTAc                                                       >  8:164693/1‑117 (MQ=255)
                                                                                                                   tggtgCACCACCTCATCCAGCTGGTTGAC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTAc                                                       <  7:164693/117‑1 (MQ=255)
                                                                                                                     gtgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGAt                                                           >  4:325668/1‑111 (MQ=255)
                                                                                                                     gtgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGAt                                                           <  3:325668/111‑1 (MQ=255)
                                                                                                                      tgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAg                    <  8:131590/149‑1 (MQ=255)
                                                                                                                      tgCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAg                    <  8:206717/149‑1 (MQ=255)
                                                                                                                            accTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGc              <  7:135586/149‑1 (MQ=255)
                                                                                                                               tCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAgg                   >  3:237101/1‑141 (MQ=255)
                                                                                                                               tCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAgg                   <  4:237101/141‑1 (MQ=255)
                                                                                                                                ccaccAGTTGATTGGCGAAAGCTTT‑GCCAACCAGCTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCa          >  3:166125/4‑149 (MQ=38)
                                                                                                                                 aTCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGc                                                               >  8:140559/1‑95 (MQ=255)
                                                                                                                                 aTCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGc                                                               <  7:140559/95‑1 (MQ=255)
                                                                                                                                 aTCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGAGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCa          <  4:306841/148‑1 (MQ=255)
                                                                                                                                    cAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATcc       <  2:250985/148‑1 (MQ=255)
                                                                                                                                     aGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATc        >  3:281479/1‑146 (MQ=255)
                                                                                                                                          gTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAg  <  4:281479/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CAGGCACGGCCAGCGCTTCCACGCCGCGCCAGTTGCCGGGCTGCTCAGCTTCTCTCGATAAGCCTCCACCGATTCAACGGCAAAGCTCTCATCATTAAGCATTTCGTCGACCGAGTGGTGCACCACCTCATCCAGCTGGTTGGC‑AAAGCTTTCGCCAATCAACTGGTGGGCAATCAGGTTGGCGACCGTGGTGTCGACCGGGATCAACGGCTGCTGGAAGTGGCGGATGTACAAGTCATTCACCTCTTCCACCAGGCGGTGCGCCAGGTAGGCTTCATCCAGAAG  >  NC_002947/107838‑108122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑